Accidental contamination of diagnostic cultures of mycobacteria and phage identification of the contaminating strain

Sŭla, L; Sulová, J

doi:10.1016/0041-3879(79)90016-3

Cited by 3 publications

(3 citation statements)

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“…New molecular techniques for strain differentiation (Burman et al, 1997; Poynten et al, 2002; Small et al, 1993; Bauer et al, 1997; de Ramos et al, 1999; Nivin et al, 2000; Filho et al, 2002; Gascoyne-Binzi et al, 2001) with superior sensitivity and discriminatory power have rendered conventional methods such as phage testing (Sula and Sulova 1979; Maurer et al, 1984; Jones 1988) obsolete. Spoligotyping is a useful 1st-line tool (de Ramos et al, 1999; Nivin et al, 2000) in a 2-stage algorithm (as used in this study) in which subsequent RFLP examination may enhance discrimination of suspicious strains sharing the same spoligotype.…”

Section: Discussionmentioning

confidence: 99%

Infrequent MODS TB culture cross-contamination in a high-burden resource-poor setting

Moore¹,

Caviedes²,

Gilman³

et al. 2006

Diagnostic Microbiology and Infectious Disease

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One obstacle to wider use of rapid liquid culture-based tuberculosis diagnostics such as the microscopic observation drug susceptibility (MODS) assay is concern about cross-contamination. We investigated the rate of laboratory cross-contamination in MODS, automated MBBacT, and Lowenstein-Jensen (LJ) cultures performed in parallel, through triangulation of microbiologic (reculturing stored samples), molecular (spoligotype/RFLP), and clinical epidemiologic data. At least 1 culture was positive for Mycobacterium tuberculosis for 362 (11%) of 3416 samples; 53 were regarded as potential cross-contamination suspects. Cross-contamination accounted for 17 false-positive cultures from 14 samples representing 0.41% (14/3416) and 0.17% (17/10 248) of samples and cultures, respectively. Positive predictive values for MODS, MBBacT (bioMérieux, Durham, NC), and LJ were 99.1%, 98.7%, and 99.7%, and specificity was 99.9% for all 3. Low rates of cross-contamination are achievable in mycobacterial laboratories in resource-poor settings even when a large proportion of samples are infectious and highly sensitive liquid culture-based diagnostics such as MODS are used. Europe PMC Funders Group Europe PMC Funders Author ManuscriptsEurope PMC Funders Author Manuscripts BackgroundIn mycobacteriology reference-level laboratories in the industrialized world, crosscontamination is estimated to account for between 0.5% and 6% of positive results (Bauer et al., 1997;Nivin et al., 1998;Burman and Reves 2000;de Boer et al., 2002;Jasmer et al., 2002;Ruddy et al., 2002) with significant associated cost implications (Northrup et al., 2002), and higher levels might be anticipated in high tuberculosis (TB)-burden resourcelimited settings where laboratory facilities are less sophisticated and a greater proportion of samples contain Mycobacterium tuberculosis. The microscopic observation drug susceptibility (MODS) assay in which 2 sputum samples are cultured on the same 24-well tissue-culture plate in Middlebrook 7H9 medium (Caviedes et al., 2000;Moore et al., 2004) has been proposed as a potential tool to bring low-tech sensitive TB diagnosis to the developing world where the need is most urgent; however, the risk of cross-contamination in this simple low-tech method using liquid culture medium and multiple samples in a single 24-well plate has not been previously determined.Examining samples obtained in a large community-based study in urban Lima, Peru, we determined the rate of M. tuberculosis cross-contamination in sputum cultures performed in parallel in MODS, MBBacT-automated mycobacterial culture system (bioMérieux, Durham, NC), and Lowenstein-Jensen (LJ) solid media by first defining and then investigating contamination suspect positive cultures. Patients, materials, and methods Sample collectionAfter written informed consent, 1923 patients undergoing investigation for TB at health centers in Lima, Peru, were recruited over an 18-month period into an operational evaluation of the MODS assay. Two sputum samples were requested from e...

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Section: Discussionmentioning

confidence: 99%

Infrequent MODS TB culture cross-contamination in a high-burden resource-poor setting

Moore¹,

Caviedes²,

Gilman³

et al. 2006

Diagnostic Microbiology and Infectious Disease

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“…Despite its low discriminatory power, phage typing was applied for this purpose (459). In the early years of molecular typing by the IS6110-RFLP method, a series of studies examined its role in the identification of cross-contamination.…”

Section: Applications Of Strain Typing To M Tuberculosis Complex Isomentioning

confidence: 99%

Methodological and Clinical Aspects of the Molecular Epidemiology of Mycobacterium tuberculosis and Other Mycobacteria

et al. 2016

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SUMMARYMolecular typing has revolutionized epidemiological studies of infectious diseases, including those of a mycobacterial etiology. With the advent of fingerprinting techniques, many traditional concepts regarding transmission, infectivity, or pathogenicity of mycobacterial bacilli have been revisited, and their conventional interpretations have been challenged. Since the mid-1990s, when the first typing methods were introduced, a plethora of other modalities have been proposed. So-called molecular epidemiology has become an essential subdiscipline of modern mycobacteriology. It serves as a resource for understanding the key issues in the epidemiology of tuberculosis and other mycobacterial diseases. Among these issues are disclosing sources of infection, quantifying recent transmission, identifying transmission links, discerning reinfection from relapse, tracking the geographic distribution and clonal expansion of specific strains, and exploring the genetic mechanisms underlying specific phenotypic traits, including virulence, organ tropism, transmissibility, or drug resistance. Since genotyping continues to unravel the biology of mycobacteria, it offers enormous promise in the fight against and prevention of the diseases caused by these pathogens. In this review, molecular typing methods forMycobacterium tuberculosisand nontuberculous mycobacteria elaborated over the last 2 decades are summarized. The relevance of these methods to the epidemiological investigation, diagnosis, evolution, and control of mycobacterial diseases is discussed.

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“…CF is the third most common indication for lung transplantation, but persistent infection – particularly when NTM are present prior to surgery – can result in substantial post-transplant morbidity and mortality4 , 6 , 7. Therapeutic bacteriophages are a plausible alternative treatment to antibiotics8, but have not been used for mycobacterial infections in humans9 – 11; personalized intravenous phage treatments for other bacterial infections have been described (Supplemental Information)12 , 13.…”

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confidence: 99%

Engineered bacteriophages for treatment of a patient with a disseminated drug-resistant Mycobacterium abscessus

Dedrick

Bustamante

Garlena

et al. 2019

Nat Med

992

872

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A 15-year-old cystic fibrosis patient with a disseminated Mycobacterium abscessus infection was treated with a three-phage cocktail following bilateral lung transplantation. Effective lytic phage derivatives that efficiently kill the infectious M. abscessus strain were developed by genome engineering and forward genetics. Intravenous phage treatment was well tolerated and associated with objective clinical improvement including sternal wound closure, improved liver function, and substantial resolution of infected skin nodules.

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Accidental contamination of diagnostic cultures of mycobacteria and phage identification of the contaminating strain

Cited by 3 publications

References 1 publication

Infrequent MODS TB culture cross-contamination in a high-burden resource-poor setting

Infrequent MODS TB culture cross-contamination in a high-burden resource-poor setting

Methodological and Clinical Aspects of the Molecular Epidemiology of Mycobacterium tuberculosis and Other Mycobacteria

Engineered bacteriophages for treatment of a patient with a disseminated drug-resistant Mycobacterium abscessus

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