Accessing the T-Cell and B-Cell Immuno-Dominant Peptides from A.baumannii Biofilm Associated Protein (bap) as Vaccine Candidates: A Computational Approach

Sinduja

et al. 2021

JPRI

Introduction: High fat induced (HFI) liver is associated with a high calorie intake and many other diet-induced complications, such as metabolic syndrome and cardiovascular disease. HFI is the most common chronic liver disease that develops due to high fat intake. A wide range of liver injuries is associated with HFI, ranging from simple steatosis to nonalcoholic steatohepatitis (NASH), advanced fibrosis and cirrhosis. The signs and symptoms of metabolic syndrome may be induced in rats by feeding them a diet rich in carbohydrates and fat. The aim of the study is Comparison of efficacy of the PAP (Papanicolaou) stain and H&E (Hematoxylin and eosin) stain in identifying the various changes in liver samples of high fat diet induced rat. Materials and Methods: Liver samples of Normal control group animal and High fat diet induced animal were sectioned, weighed fixed 10% neutral buffer formalin and mounted in DPX compound. Four micrometer sections were stained with hematoxylin-eosin and PAP stains for the histological examination of micro-vesicular and macro-vesicular steatosis in the liver tissue. The grading of steatosis was given from 0-3 based on the lipid accumulation (steatosis): grade 0 - absence; grade 1 - mild Steatosis (<30% hepatocyte); grade 2- moderate steatosis (30%-70% hepatocyte); grade3- severe steatosis (>70%hepatocytes). Efficacy of grade of PAP stain and H&E stain were accessed and statistically evaluated by considering seven parameters and overall staining characters where compared. Results: Our present study evaluated high fat induced hepatic steatosis of H&E and PAP stain and also evaluated efficacy of both the stain. In our study, Pie chart showing the percentage distribution of Number of micro-vesicles about 70% represents greater than 30, 10% represents 30-70, 20% represents less than 70 (Fig. 1). In our study 20% represents the greater than 30, 30% represents the 30-70, 50% represents Less than 70 (Fig. 2). 30% represents mild steatosis, 20% represents moderate steatosis, 50% represents Severe Steatosis (Fig. 3). Conclusion: We demonstrated the comparative efficiency of the PAP and H&E high fat diet induced rat liver by in vivo and conclude that both H&E and PAP stain has equal efficacy and clarity in observing the various minute parameters.

Section: Discussionmentioning

confidence: 99%

Comparison of Efficacy of the Pap and H & E in Identifying the Various Changes in Liver Samples of High Fat Diet Induced Rat - In vitro Study

Sri

Sinduja

et al. 2021

JPRI

“…As of now, the awareness among the females of the 20-40 age group is higher than the 40-60 years age group. There is a need to create general awareness irrespective of the age group in future [42][43][44][45]. There should be spread of awareness through social media, health camps, polls, etc., to prevent the increase of cancer rate per year worldwide.…”

Section: Discussionmentioning

confidence: 99%

Comparison on Awareness of Radiation Hazards among Females of Different Age Group

Joshua

Sinduja

2021

JPRI

Background: Radiation is a source of energy which travels through materials. The ionising radiation produce free radicals from an atom with the electron removal. X-ray is the most common ionizing radiation helping in diagnosis. There is an increase in the use of radiation in diagnosis, procedural along with surgical treatment where radiologists and the patients should be aware of the radiation hazards and its ill effects. The aim of our study is to analyse the radiation hazards awareness among females of various age groups. Materials and Methods: The study was done as an online setting; the responses were obtained from 103 female participants from the Chennai sub population. A self-structured questionnaire comprising about 18 yes or no types of questions were prepared in google forms and circulated as a link to the participants. The responses were exported to google sheets and data was retrieved and analysed in SPSS software version 26. The results were obtained from SPSS software. Results: From the results analysed, 74.76% have undergone X ray or any procedures but only few (25.24%) did not undergo any diagnostic procedures. 71.84% of them were aware that all radiation is hazardous whereas the rest (28.16%) were not aware. 88.35% of them accepted that bone marrow and child’s thyroid tissue are more sensitive to radiation but few (11.65%) did not accept it. 71.84% of the participants of females of age group 20-40 years were more aware than females of 40-60 years age group (16.50%) that bone marrow, the child's thyroid is the sensitive tissue affected by radiation. Chi square test was done and the association was found to be not statistically significant with P-value .236 (P = .05). Chi square test was done to check awareness of thyroid cancer caused mainly through radiations between the two age groups of females and the association was found to be not statistically significant with p-value is .703 (P = .05). Conclusion: Within the limits of the study, the females of age group 20-40 years have higher levels of awareness than females of age group 40-60 years.

“…B-cell or antibodydeficiency disorders are the most common types. The mainstay of treatment for patients with these disorders is immunoglobulin replacement therapy, and there are now several Ig products approved around the world for patients with immunodeficiency [41][42][43].…”

Section: Mechanism Of Chemotherapy-induced Immunodeficiencymentioning

confidence: 99%

Mechanism of Common Systemic Immunodeficiency Disorders and its Literature Comparison

Ruskin

Sindhuja

2021

JPRI

Systemic immunodeficiency disorders are heterogenous groups ofImmunodeficiency disorders could experience an assortment of clinical signs, including intermittent, extreme, or irregular diseases, autoimmunity, and lymphoproliferative/malignancies. Immunodeficiency involves an enormous amount of sicknesses, influencing the advancement of the immune system, its function, or both. There is a increase in percentage of immunodeficiency disorders among population. However, numerous patients are diagnosed late; numerous cases experience the ill effects of difficulties by chronic infections, end-organ damage, or even demise before the diagnosis is made. Ideal determination and suitable treatment remain key to the successful management of patients. The objective of this review is to overview the various systemic immunodeficiency disorders and their mechanism of occurrence of immunodeficiency.