Abstract:The purpose of this study was to evaluate the accuracy of 2 newly developed physical activity questionnaires: the Canadian Society for Exercise Physiology (CSEP) Physical Activity and Sedentary Behaviour Questionnaire (PASB-Q) and a newly modified Leisure-Time Physical Activity Questionnaire (mLTPA-Q). These questionnaires were compared with objective measurements of physical activity and fitness (accelerometry and physiological assessments) in 35 adults, before and after a week of daily living activity. Objectively measured moderate-to vigorous-intensity aerobic physical activity (MVPA) was moderately correlated with the PASB-Q's physical activity vital sign (PAVS) (r = 0.50, p = 0.004) and the mLTPA-Q (r = 0.56, p = 0.001). Bland−Altman plots suggest minimal bias from self-reported to objective measures of MVPA. The ability of PAVS to accurately distinguish who does and does not achieve Canadian physical activity guidelines was 83% and 60%, respectively, compared with 82% and 73% of the mLTPA-Q. Self-reported sedentary time was greatly underestimated in the PASB-Q compared with the objective measure (6.4 ± 3.5 vs 12.2 ± 1.2 h/day). The results of this study suggest the PASB-Q and mLTPA-Q are valid and reliable measures of adult physical activity and provide reasonable indication of those individuals who meet physical activity guidelines. Future questionnaire development should take into account the underestimation of time spent engaging in sedentary activities.Key words: physical activity, sedentary behaviour, accelerometry, physical activity questionnaires, validity, reliability. Mots-clés : activité physique, comportement sédentaire, accélérométrie, questionnaires sur l'activité physique, validité, fiabilité.