Acceleration of experimental lapine osteoarthritis by calcium pyrophosphate microcrystalline synovitis

Abstract

Objective. To investigate the effects of chronic calcium pyrophosphate dihydrate (CPPD) synovitis on the development of osteoarthritic (OA) lesions in an animal model.Methods. OA was induced in the right knees of 30 male New Zealand white rabbits by partial lateral meniscectomy and section of the fibular collateral and sesamoid ligaments (PLWLS), followed by 8 weekly intraarticular (IA) injections of 1 mg (low-dose) or 10 mg (high-dose) of CPPD crystals in 3 sets of experiments (10 rabbits each). The contralateral left knees served as controls: experiment 1 PLMLS alone, experiment 2 8 weekly IA injections of CPPD crystals alone, and experiment 3 sham surgery plus 8 weekly IA injections of CPPD crystals.Results. At 8 weeks, repeated IA injections of low-dose and high-dose CPPD crystals into meniscectomized right knees resulted in more severe OA than in meniscectomized but noninjected left knees (experiment 1) (P = 0.003 and P = 0,001, respectively). One-fourth of the meniscectomized knees (11 of 40), both CPPDinjected and noninjected, showed embedded synovial cartilage shards.Conelusion. The data demonstrate a worsening effect of chronic CPPD crystal-induced synovitis on experimental OA produced in the rabbit knees by

The murine homolog of the interleukin-8 receptor CXCR-2 is essential for the occurrence of neutrophilic inflammation in the air pouch model of acute urate crystal-induced gouty synovitis

“…Studies to further elucidate the mechanism and biologic significance of the ability of colchicine to suppress neutrophil responsiveness to IL-8 in vitro (13), and its ability to suppress phagocyte release of crystal-induced " C C F activity (55) and certain other inflammatory cytokines (56), should be of interest. Our results also suggest that more selective inhibition of expression of IL-8 receptor-binding C-X-C chemokines, or blockade of neutrophil responses to these neutrophil chemotaxins, may provide supplemental means to reduce the frequency, intensity, and degradative sequelae (1,39,57) of recurrent crystal-induced articular inflammation.…”

Role of S100A8 and S100A9 in neutrophil recruitment in response to monosodium urate monohydrate crystals in the air‐pouch model of acute gouty arthritis

“…All of these chemokines had previously been shown to play a role in neutrophil migration in the air-pouch model in response to both TNF␣ and SEA (39,40). The air-pouch model was selected since it has been used extensively in the past as a model of the synovial environment to study the mechanism of inflammation induced by different proinflammatory factors, including MSU (45)(46)(47)(48)(49)(50)(51). Designed to replicate the synovial lining, this model allows for the identification and study of emigrated leukocytes and molecules produced during an inflammatory response, although several features of the synovial environment are missing in air-pouch fibroblasts (52,53).…”

The Role of Uric Acid and Other Crystals in Osteoarthritis

“…Such a hypothesis is supported by a study in which rabbit joints were subjected to intra-articular CPP crystal injections after meniscectomy, resulting in severe OA. In contrast, rabbits with healthy (ie, nonmeniscetomized) joints did not develop OA despite CPP injection [43].…”