Acceleration of autologous in vivo recellularization of decellularized aortic conduits by fibronectin surface coating

Assmann, Alexander; Delfs, Christofer; Munakata, Hiroshi; Schiffer, Franziska; Horstkötter, Kim; Huynh, Khon; Barth, Mareike; Stoldt, Volker R.; Kamiya, Hiroyuki; Boeken, Udo; Lichtenberg, Artur; Akhyari, Payam

doi:10.1016/j.biomaterials.2013.04.037

Cited by 104 publications

(117 citation statements)

References 27 publications

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“…This is consistent with previous in vivo findings, in which adventitial bioactive coating of dAoGs stimulated repopulation of the media region 21 and luminal fibronectin or RGD coating triggered neointima formation. 21,49 Nonetheless, further studies are warranted, evaluating the possible effects of customized biofunctionalization of dECM on in vivo endothelialization.…”

Section: Evaluation Of Biological Effects Of Functionalization Of Decsupporting

confidence: 93%

“…21 Briefly, cryosections were incubated with 10 mg/mL fluorescein-labeled gelatin (Invitrogen) in 50 mM Tris-HCl, 10 mM CaCl 2 , 150 mM NaCl, and 5% Triton X-100 for 20 h at room temperature. Sections were mounted with VECTASHIELD medium containing DAPI, and MMP gelatinase activity was visualized by fluorescence microscopy imaging as described above.…”

Section: In Vivo Endothelialization Studiesmentioning

confidence: 99%

“…21 However, protein-based coatings may prove insufficient to selectively elicit specific biological cues potentially required in the process of neoendothelium formation, thus failing in providing functional endothelium. 21 This is concordant with the intrinsic limitations associated with the use of full-length proteins as coating molecules, which include low specificity, limited stability, and immunogenicity. 24 Thus, newer strategies aiming at circumventing the limitations of classical protein-based methods are warranted in the design of functional biological grafts.…”

Section: Evaluation Of Biological Effects Of Functionalization Of Decmentioning

confidence: 99%

“…[15][16][17][18] In the past, surface coating of engineered vascular grafts with bioactive proteins, such as fibronectin, fibrin, or vascular endothelial growth factor, has been shown to accelerate the autologous recellularization and improve graft function. [19][20][21] However, protein-based strategies may not lead to functional endothelium or prevent neointimal hyperplasia as they have been proven insufficient to elicit specific biological cues required in the process of neoendothelium formation. 21 In particular, a biological blood-graft interface with high endothelial cell (EC) specificity, while suppressing activation of platelet attachment, immune cell recruitment, and smooth muscle cell overproliferation, is crucial in the process of in vivo endothelialization.…”

Section: Introductionmentioning

confidence: 99%

“…[19][20][21] However, protein-based strategies may not lead to functional endothelium or prevent neointimal hyperplasia as they have been proven insufficient to elicit specific biological cues required in the process of neoendothelium formation. 21 In particular, a biological blood-graft interface with high endothelial cell (EC) specificity, while suppressing activation of platelet attachment, immune cell recruitment, and smooth muscle cell overproliferation, is crucial in the process of in vivo endothelialization. 22 In this regard, cell-specific surface functionalization can be achieved using short synthetic peptides with defined bioactive sequences.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Customized Interface Biofunctionalization of Decellularized Extracellular Matrix: Toward Enhanced Endothelialization

Aubin

Mas‐Moruno

Iijima

et al. 2016

Tissue Engineering Part C: Methods

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Interface biofunctionalization strategies try to enhance and control the interaction between implants and host organism. Decellularized extracellular matrix (dECM) is widely used as a platform for bioengineering of medical implants, having shown its suitability in a variety of preclinical as well as clinical models. In this study, specifically designed, custom-made synthetic peptides were used to functionalize dECM with different cell adhesive sequences (RGD, REDV, and YIGSR). Effects on in vitro endothelial cell adhesion and in vivo endothelialization were evaluated in standardized models using decellularized ovine pulmonary heart valve cusps (dPVCs) and decellularized aortic grafts (dAoGs), respectively. Contact angle measurements and fluorescent labeling of custommade peptides showed successful functionalization of dPVCs and dAoGs. The functionalization of dPVCs with a combination of bioactive sequences significantly increased in vitro human umbilical vein endothelial cell adhesion compared to nonfunctionalized controls. In a functional rodent aortic transplantation model, fluorescent-labeled peptides on dAoGs were persistent up to 10 days in vivo under exposure to systemic circulation. Although there was a trend toward enhanced in vivo endothelialization of functionalized grafts compared to nonfunctionalized controls, there was no statistical significance and a large biological variability in both groups. Despite failing to show a clear biological effect in the used in vivo model system, our initial findings do suggest that endothelialization onto dECM may be modulated by customized interface biofunctionalization using the presented method. Since bioactive sequences within the dECM-synthetic peptide platform are easily interchangeable and combinable, further control of host cell proliferation, function, and differentiation seems to be feasible, possibly paving the way to a new generation of multifunctional dECM scaffolds for regenerative medicine.

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Section: Evaluation Of Biological Effects Of Functionalization Of Decsupporting

confidence: 93%

Section: In Vivo Endothelialization Studiesmentioning

confidence: 99%

Section: Evaluation Of Biological Effects Of Functionalization Of Decmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Customized Interface Biofunctionalization of Decellularized Extracellular Matrix: Toward Enhanced Endothelialization

Aubin

Mas‐Moruno

Iijima

et al. 2016

Tissue Engineering Part C: Methods

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A Highly Elastic and Rapidly Crosslinkable Elastin‐Like Polypeptide‐Based Hydrogel for Biomedical Applications

Zhang

Avery

Vallmajó-Martín

et al. 2015

Adv Funct Materials

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205

177

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Elastin-like polypeptides (ELPs) are promising for biomedical applications due to their unique thermoresponsive and elastic properties. ELP-based hydrogels have been produced through chemical and enzymatic crosslinking or photocrosslinking of modified ELPs. Herein, a photocrosslinked ELP gel using only canonical amino acids is presented. The inclusion of thiols from a pair of cysteine residues in the ELP sequence allows disulfide bond formation upon exposure to UV light, leading to the formation of a highly elastic hydrogel. The physical properties of the resulting hydrogel such as mechanical properties and swelling behavior can be easily tuned by controlling ELP concentrations. The biocompatibility of the engineered ELP hydrogels is shown in vitro as well as corroborated in vivo with subcutaneous implantation of hydrogels in rats. ELP constructs demonstrate long-term structural stability in vivo, and early and progressive host integration with no immune response, suggesting their potential for supporting wound repair. Ultimately, functionalized ELPs demonstrate the ability to function as an in vivo hemostatic material over bleeding wounds.

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Tissue Engineered Bio‐Blood‐Vessels Constructed Using a Tissue‐Specific Bioink and 3D Coaxial Cell Printing Technique: A Novel Therapy for Ischemic Disease

Gao

Lee

Jang

et al. 2017

Adv Funct Materials

237

171

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Endothelial progenitor cells (EPCs) are a promising cell source for the treatment of several ischemic diseases for their potentials in neovascularization. However, the application of EPCs in cell-based therapy has shown low therapeutic efficacy due to hostile tissue conditions after ischemia. In this study, a bio-blood-vessel (BBV) is developed, which is produced using a novel hybrid bioink (a mixture of vascular-tissue-derived decellularized extracellular matrix (VdECM) and alginate) and a versatile 3D coaxial cell printing method for delivering EPC and proangiogenic drugs (atorvastatin) to the ischemic injury sites. The hybrid bioink not only provides a favorable environment to promote the proliferation, differentiation, and neovascularization of EPCs but also enables a direct fabrication of tubular BBV. By controlling the printing parameters, the printing method allows to construct BBVs in desired dimensions, carrying both EPCs and atorvastatin-loaded poly(lactic-co-glycolic) acid microspheres. The therapeutic efficacy of cell/drug-laden BBVs is evaluated in an ischemia model at nude mouse hind limb, which exhibits enhanced survival and differentiation of EPCs, increased rate of neovascularization, and remarkable salvage of ischemic limbs. These outcomes suggest that the 3D-printed ECM-mediated cell/drug implantation can be a new therapeutic approach for the treatment of various ischemic diseases.

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Acceleration of autologous in vivo recellularization of decellularized aortic conduits by fibronectin surface coating

Cited by 104 publications

References 27 publications

Customized Interface Biofunctionalization of Decellularized Extracellular Matrix: Toward Enhanced Endothelialization

Customized Interface Biofunctionalization of Decellularized Extracellular Matrix: Toward Enhanced Endothelialization

A Highly Elastic and Rapidly Crosslinkable Elastin‐Like Polypeptide‐Based Hydrogel for Biomedical Applications

Tissue Engineered Bio‐Blood‐Vessels Constructed Using a Tissue‐Specific Bioink and 3D Coaxial Cell Printing Technique: A Novel Therapy for Ischemic Disease

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