“…Due to its low permeability the barrier to the osmotic solution, both water and substances dissolving in vacuolar sap increases (Kucner et al, 2013). Because of the slowness in solid-liquid mass transfer, several methods have been considered to accelerate the kinetics of this process (Cárcel, et al, 2007;Rastogi et al, 2002), among others the application of pulsed vacuum (Escriche et al, 2000), centrifugal forces (Azuara et al, 1996), electrical fields (Ade-Omowaye et al, 2003;Rastogi et al, 1999) or high intensity ultrasound (Cárcel, et al, 2007;Kowalski and Szadzińska, 2014;Mulet et al, 2003;Siucińska and Konopacka, 2014).…”