Accelerated In Vitro Degradation of Optically Clear Low–β Sheet Silk Films by Enzyme-Mediated Pretreatment

Zarbin, Marco A.

doi:10.1001/jamaophthalmol.2013.4319

Cited by 6 publications

(3 citation statements)

References 28 publications

(49 reference statements)

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“…The Rq for KL, TN, and TS was found to be in the range of 2.33 to 2.66, with no significant differences among the three sources. The findings in the current study were relatable to the smoothness factors reported earlier by other groups 16 . As for the physical properties, in the current study, KL, TN, TS derived films exhibited a contact angle of about 77–80°, water uptake% of about 18%–21%, swelling ratio of 1.18–1.22 and WVT% of 19%–21%.…”

Section: Discussionsupporting

confidence: 83%

“…The findings in the current study were relatable to the smoothness factors reported earlier by other groups. 16 As for the physical properties, in the current study, KL, TN, TS derived films exhibited a contact angle of about 77-80°, water uptake% of about 18%-21%, swelling ratio of 1.18-1.22 and WVT% of 19%-21%. There was no evidence of source-dependent variations in the physical properties of the SF films.…”

Section: Cytocompatibility Featuresmentioning

confidence: 51%

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A study on source dependent batch to batch variations in silk fibroin films for potential applications in corneal tissue engineering

Dan

Singh

et al. 2023

MedComm – Biomaterials and Applications

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The demand‐to‐supply gap, rejection rates, and the chances of infection associated with organ/tissue transplantation prompted researchers to find alternative solutions such as tissue engineering. Here, healthy cells are cultured over a biomaterial framework supplemented with growth factors to create bioartificial tissues. As a scaffolding biomaterial, silk fibroin (SF), a biopolymer obtained from Bombyx mori silk cocoons, offers unique properties. However, natural polymers, including SF, were criticized for preconceived source‐dependent batch‐to‐batch variations. Therefore, this study aims to prepare B. mori SF‐based films and investigate source‐dependent variations, if any. For this purpose, we have sourced silk cocoons from three geographical locations in India and processed them into films with a solvent‐casting approach. As a whole, our results indicate that there were slight variations in the morphological features in the raw cocoon stage; however, once processed, there were no significant differences in their topological, physical, chemical, optical, mechanical, or degradable properties with respect to the source. Further, all the films were found to be noncytotoxic and cytocompatible with corneal cells in vitro. Therefore, the study indicates no source‐dependent variations in biopolymers and suggested that SF from any source can be processed into biomaterials for potential biomedical applications.

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Section: Discussionsupporting

confidence: 83%

Section: Cytocompatibility Featuresmentioning

confidence: 51%

A study on source dependent batch to batch variations in silk fibroin films for potential applications in corneal tissue engineering

Dan

Singh

et al. 2023

MedComm – Biomaterials and Applications

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“…In general, a greater crystalline content (β-sheets) provides structural integrity and enhances resistance toward degradation, while the opposite causes a less compacted network and rapid degradation . Owing to this strong correlation, strategies that can modulate the crystalline level can be used to tailor the degradation rate.…”

Section: Strategies For Controlling the Degradation Rate Of Sfmentioning

confidence: 99%

Strategies for Tuning the Biodegradation of Silk Fibroin-Based Materials for Tissue Engineering Applications

Diane

Yang

Long

et al. 2020

ACS Biomater. Sci. Eng.

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The remarkable features of silk fibroin (SF) from the silkworm (Bombyx mori) have fueled its application as a candidate biomaterial for tissue regeneration and repair. For an ideal scaffold, the rate of degradation should be synchronized to match the rate of new tissue formation, and tuning this rate is essential, as diverse tissues differ in terms of regeneration period. In this Review, we discuss the factors influencing the degradability of SF, which can vary from days to several months, depending on the state of the raw material, the scaffold preparation process, morphological features, and host factors. This knowledge facilitates strategies for tuning the SF degradation rate, including manipulation of molecular weight, crystalline level, and crosslinking degree. Since these strategies have a great influence on the mechanical properties, the superiority of SF has to be sacrificed to satisfy the requirements for degradation rate. We further explore additional strategies, including the incorporation of degradation-promoting supplements such as blending with another polymer (e.g., gelatin) and the incorporation of enzyme-sensitive peptides. The information in this Review will likely aid scientists working with SF materials for the regeneration of diverse tissues.

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Silk fibroin/vitreous humor hydrogel scaffold modified by a carbodiimide crosslinker for wound healing

Rafiei,

Ghanbari‐Abdolmaleki,

Zeinali

et al. 2024

Biopolymers

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Natural‐derived biomaterials can be used as substrates for the growth, proliferation, and differentiation of cells. In this study, bovine vitreous humor as a biological material was cross‐linked to silk fibroin with different concentration ratios to design a suitable substrate for corneal tissue regeneration. The cross‐linked samples were evaluated with different analyses such as structural, physical (optical, swelling, and degradation), mechanical, and biological (viability, cell adhesion) assays. The results showed that all samples had excellent transparency, especially those with higher silk fibroin content. Increasing the ratio of vitreous humor to silk fibroin decreased mechanical strength and increased swelling and degradation, respectively. There was no significant difference in the toxicity of the samples, and with the increase in vitreous humor ratio, adhesion and cell proliferation increased. Generally, silk fibroin with vitreous humor can provide desirable characteristics as a transparent film for corneal wound healing.

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Accelerated In Vitro Degradation of Optically Clear Low–β Sheet Silk Films by Enzyme-Mediated Pretreatment

Cited by 6 publications

References 28 publications

A study on source dependent batch to batch variations in silk fibroin films for potential applications in corneal tissue engineering

A study on source dependent batch to batch variations in silk fibroin films for potential applications in corneal tissue engineering

Strategies for Tuning the Biodegradation of Silk Fibroin-Based Materials for Tissue Engineering Applications

Silk fibroin/vitreous humor hydrogel scaffold modified by a carbodiimide crosslinker for wound healing

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