Accelerated CRISPR/Cas12a-based small molecule detection using bivalent aptamer

Li, Xiuping; Chen, Xiujin; Mao, Minxin; Peng, Chifang; Wang, Zhouping

doi:10.1016/j.bios.2022.114725

Cited by 25 publications

(16 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As shown in Figure B, the highest inhibition rate was obtained with probe 3, a result that is fully consistent with the previous conclusion. However, we found that the inhibition rate obtained was only 33% in the presence of 200 μM ATP, which was not sensitive enough compared to some other typical LFA for ATP. − …”

Section: Resultsmentioning

confidence: 61%

“…Therefore, the proposed “auxiliary hybridization” strategy is conducive to designing effective cDNA probes and developing competitive small-molecule Apt-LFA. In addition, most of the currently reported Apt-LFA assays for kanamycin require signal amplification and a cumbersome detection process (Table S4), which is not conducive to the advantages of LFA as a rapid detection method. ,,, In addition, our Apt-LFA has even higher sensitivity than them. Therefore, this method is an ideal assay.…”

Section: Resultsmentioning

confidence: 98%

“…Due to the advantages of portability and easy operation, our Apt-LFA has much high application potential. In addition, there are only three reports on Apt-LFA for ATP, and two of these methods require signal amplification ,, (Table S4). In contrast, our Apt-LFA is independent of these and achieves comparable or even higher sensitivity than these methods.…”

Section: Resultsmentioning

confidence: 99%

“…In addition, most of the currently reported Apt-LFA assays for kanamycin require signal amplification and a cumbersome detection process (Table S4), which is not conducive to the advantages of LFA as a rapid detection method. 20,25,42,47 In addition, our Apt-LFA has even higher sensitivity than them. Therefore, this method is an ideal assay.…”

mentioning

confidence: 86%

See 3 more Smart Citations

A Versatile Strategy of Designing Gold Nanoparticle–cDNA Nanoprobes in Developing Aptamer-Based Lateral Flow Assay for Small-Molecule Detection

Sun

Chang

et al. 2023

Langmuir

Self Cite

View full text Add to dashboard Cite

Aptamer-based lateral flow assay (Apt-LFA) has shown promising applications for small-molecule detection. However, the design of the AuNP (gold nanoparticle)−cDNA (complementary DNA) nanoprobe is still a big challenge due to the moderate affinity of the aptamer to small molecules. Herein, we report a versatile strategy to design a AuNPs@polyA−cDNA (poly A, a repeat sequence with 15 A bases) nanoprobe for small-molecule Apt-LFA. The AuNPs@polyA−cDNA nanoprobe contains a polyA anchor blocker, complementary DNA segment to DNA on the control line (cDNAc), partial complementary DNA segment with aptamer (cDNAa), and auxiliary hybridization DNA segment (auxDNA). Using adenosine 5′-triphosphate (ATP) as a model target, we optimized the length of auxDNA and cDNAa and achieved a sensitive detection of ATP. In addition, kanamycin was used as a model target to verify the universality of the concept. Therefore, this strategy can be easily extended to other small molecules; therefore, high application potential in Apt-LFAs can be envisaged.

show abstract

Section: Resultsmentioning

confidence: 61%

Section: Resultsmentioning

confidence: 98%

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 86%

See 2 more Smart Citations

A Versatile Strategy of Designing Gold Nanoparticle–cDNA Nanoprobes in Developing Aptamer-Based Lateral Flow Assay for Small-Molecule Detection

Sun

Chang

et al. 2023

Langmuir

Self Cite

View full text Add to dashboard Cite

show abstract

“…As shown in Figure 6e, Li et al constructed a bivalent aptamer consisting of two aptamer strands and a linker strand, where the linker strand consisted of two short DNA fragments (cDNAs) that partially complemented the aptamer and a T-base repeat segment connecting the two cDNAs [82]. In the absence of targets, a molecular hybridization occurred between the bivalent aptamer and Cas12a-crRNA, activating the trans-cleavage activity of Cas12a to cleave biotin-modified ssDNAs (Bio-ssDNAs), and the cleaved Bio-ssDNA strands could not bind to the Au NPs@polyA-DNA probes.…”

Section: Detection Of Low Molecular Weight Substancesmentioning

confidence: 99%

Review on the Selection of Aptamers and Application in Paper-Based Sensors

Wang

et al. 2022

Biosensors

View full text Add to dashboard Cite

An aptamer is a synthetic oligonucleotide, referring to a single-stranded deoxyribonucleic acid or ribonucleic acid ligand produced by synthesis from outside the body using systematic evolution of ligands by exponential enrichment (SELEX) technology. Owing to their special screening process and adjustable tertiary structures, aptamers can bind to multiple targets (small molecules, proteins, and even whole cells) with high specificity and affinity. Moreover, due to their simple preparation and stable modification, they have been widely used to construct biosensors for target detection. The paper-based sensor is a product with a low price, short detection time, simple operation, and other superior characteristics, and is widely used as a rapid detection method. This review mainly focuses on the screening methods of aptamers, paper-based devices, and applicable sensing strategies. Furthermore, the design of the aptamer-based lateral flow assay (LFA), which underlies the most promising devices for commercialization, is emphasized. In addition, the development prospects and potential applications of paper-based biosensors using aptamers as recognition molecules are also discussed.

show abstract

Bottom‐up DNA nanostructure‐based paper as point‐of‐care diagnostic: From method to device

Gao,

Feng,

Deng

et al. 2024

Interdisciplinary Medicine

View full text Add to dashboard Cite

Paper‐based devices have attracted considerable attention in point‐of‐care testing (POCT) due to their simple operation and portability. The performance of paper‐based POC assays is further enhanced by coupling with functional nucleic acids (FNAs), which are able to selectively recognize and bind to targets, amplify and transduce signals. Several high‐performance paper‐based POC assays have been developed, resulting from the different spatial structures of the paper‐based device and detection strategies using different FNAs. In this review, we first introduce FNAs and paper‐based devices, including their concepts, classifications and advances. The following section focuses on the application of these FNAs in POC assays using paper‐based devices, taking into account their spatial one‐, two‐ and three‐dimensional structures. Finally, the challenges and perspectives of the application of FNAs in paper‐based POCT are discussed.

show abstract

Accelerated CRISPR/Cas12a-based small molecule detection using bivalent aptamer

Cited by 25 publications

References 46 publications

A Versatile Strategy of Designing Gold Nanoparticle–cDNA Nanoprobes in Developing Aptamer-Based Lateral Flow Assay for Small-Molecule Detection

A Versatile Strategy of Designing Gold Nanoparticle–cDNA Nanoprobes in Developing Aptamer-Based Lateral Flow Assay for Small-Molecule Detection

Review on the Selection of Aptamers and Application in Paper-Based Sensors

Bottom‐up DNA nanostructure‐based paper as point‐of‐care diagnostic: From method to device

Contact Info

Product

Resources

About