Ação do extrato etanólico da casca do pequi (Caryocar brasiliense) na cardiotoxicidade crônica induzida por doxorrubicina em ratos

Abstract

ABSTRACT.-Moura L.R., Orpinelli S.R.T., Sousa J.H., Faleiro M.B.R., Conceição E. Goiânia, Doxorubicin (DOX) is a chemotherapic drug used in the treatment of malignancies, but has the cardiotoxicity as collateral effect. The objective of this study was to evaluate the action of pequi shell etanolic extract (Caryocar brasiliense) (PSEE) through morphological evaluation (macroscopic, microscopic and ultramicroscopic), and to evaluate the expression of metalloproteinases (MMP2 and MMP9) and its tissue inhibitors (TIMP1 and TIMP2) in the myocardium of rats with chronic cardiotoxicity by DOX and treated or not with PSEE. The experiment lasted three months and 30 Wistar rats were divided into six groups of five animals. G1 and G2 received 300mg/kg and 600mg/kg of PSEE, respectively, as pretreatment, by gavage for seven days and continued treatment for 21 days of application of DOX. In G1, G2, G3, G4 and GC, cardiotoxicity was induced with weekly applications of 2mg/kg DOX, intraperitoneally, totaling four applications (8 mg/kg), and in the Sham group (GS) 1ml of saline solution was applied. G3 animals received daily 300mg/ kg of PSEE, and G4, 600mg/kg, by gavage, for 21 days of application of DOX. The GC and GS received 1ml of water daily by gavage also. After the completion of the application, the animals were kept for two months, with three months of experiment. Macroscopic evaluation was performed after 90 days, at which time samples were taken for analysis in electron microscopy, histopathology and immunohistochemistry. At necropsy, ascites was observed in animals that received DOX. There was a low mortality rate (3.33%), being one mouse that developed false road pneumonia. There was no change in weights and measures of the rat hearts. At doses of 300 and 600mg/kg, the PSEE attenuates myocyte vacuolar degeneration. At a dose of 600mg/kg, PSEE reduces amount Anitschkow cells. There was no significant result on the immunostaining of MMP, but considering their inhibitors (TIMP) there was a greater immunostaining of TIMP2 in GC, the group that received only DOX. It was concluded that PSEE is effective in minimizing effects of chronic cardiotoxicity induced by DOX in the myocardium of rats, whereas at doses of 300 and 600mg/ 1 Recebido em 13 de abril de 2016.Aceito para publicação em 12 de setembro de 2016.

Effect of pequi shell ethanolic extract on glutathione reductase activity in rats exposed to doxorubicin cardiotoxicity

“…The present study did not evaluate the rat heart morphology. However, we used the same extract as well as a similar methodology to that described by Moura et al (2017), who have proved the beneficial effects of PSEE on myocardial cells in the presence of DOX. Therefore, we considered that the results of the present study would reflect positively on deleterious effects of DOX on rat myocardium.…”

“…To obtain PSEE, pequi fruit peels were extracted according to a technique described by Moura et al (2017). Following extraction, the PSEE was stored at -20 °C until use.…”

Effect of the ethanolic extract of pequi (Caryocar brasiliense) peel on acute cardiotoxicity induced by doxorubicin in Wistar rats (Rattus norvegicus albinus)

“…The EEPP was processed from fruits according to a technique described by Moura et al (2017). DOX was applied intraperitoneally with catheter number 22 after antisepsis with iodopovidone.…”

Revisão integrativa do uso do óleo do pequi (Caryocar brasiliense) no processo de cicatrização de feridas cutâneas em ratos

“…Algumas pesquisas (Oliveira, C. S. et al 2017;Moura, L. R. et al, 2017;Palmeira, S. M. et al 2015) apontam que a polpa do C. brasiliense apresenta atividade in vivo e in vitro. Os extratos aquoso e alcoólico da polpa e da amêndoa foram avaliados através de testes de DPPH, ORAC, ABTS, Rancimat e cooxidação do β-caroteno/ ácido linoleico.…”