Ac transposition in transgenic tomato plants

Yoder, John I.; Palys, Joe; Alpert, Kevin B.; Lassner, Michael

doi:10.1007/bf00339594

Cited by 169 publications

(87 citation statements)

References 19 publications

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“…The transposition frequency of Ac in tobacco (17-36~o) is in agreement with the data from published phenotypic assays [2] and analysis of the DNA of transgenic plants containing the element [30], but slightly below the values reported from phenotypic assays with Solanum tuberosum [21] and DNA analysis in Lyeopersicon esculentum [ 35 ], and our unpublished results. This allows the conclusion that leaf disc transformation, combined with a phenotypic assay based on restored hygromycin resistance can be applied to determine the capacity of transposable elements to excise in different plant species.…”

Section: Discussionsupporting

confidence: 91%

“…To extend the possibilities of transposon tagging to other plant species experiments have been performed to test the activity of the maize transposable element Ac in heterologous plant species. It has been shown that the maize Ac element can transpose when it is introduced into Nicotiana tabacum, Daucus carota, Arabidopsis thaliana, Solanum tuberosum and Lycopersicon esculentum by Agrobacterium-mediated transformation [1,19,33,35].…”

Section: Introductionmentioning

confidence: 99%

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A comparative study of Tam3 and Ac transposition in transgenic tobacco and petunia plants

et al. 1989

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Disclaimer/Complaints regulationsIf you believe that digital publication of certain material infringes any of your rights or (privacy) interests, please let the Library know, stating your reasons. In case of a legitimate complaint, the Library will make the material inaccessible and/or remove it from the website. Please Ask the Library: http://uba.uva.nl/en/contact, or a letter to: Library of the University of Amsterdam, Secretariat, Singel 425, 1012 WP Amsterdam, The Netherlands. You will be contacted as soon as possible. Ac excises from the HPTII gene with comparable frequencies (30~o) in both plant species. When the /~-glucuronidase (GUS) gene was used to detect transposition of Tam3, a significantly lower excision frequency (13~o) was found in both plant species. It could be shown that deletion of parts of the transposable elements Tam3 and Ac, removing either one of the terminal inverted repeats (TIR) or part of the presumptive transposase coding region, abolished the excision from the marker genes. This demonstrates that excision of the transposable element Tam3 in heterologous plant species, as documented for the autonomous element Ac, also depends on both properties. Southern blot hybridization shows the expected excision pattern and the reintegration of Tam3 and Ac elements into the genome of tobacco plants.

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Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

A comparative study of Tam3 and Ac transposition in transgenic tobacco and petunia plants

et al. 1989

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“…ActivatorlDissociation (AclDs) elements from maize have been shown to transpose in tobacco (Baker et al, 1986), tomato (Yoder et al, 1988), potato (Knapp et al, 1988), rice (Izawa et al, 1991), flax (Ellis et al, 1992), Arabidopsis, and carrot (Van Sluys et al, 1987). Similarly, fnhancerl Supressor-mutator (EnlSpm) of maize can also transpose when introduced into tobacco (Masson and Fedoroff, 1989) and potato (Frey et al, 1989), and Tam3 of snapdragon can transpose in tobacco and petunia (Haring et al, 1989).…”

Section: Introductionmentioning

confidence: 99%

Jordan, an active Volvox transposable element similar to higher plant transposons.

1993

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We have isolated a 1595-bp transposable element from the multicellular green alga Volvox carteri following its insertion into the nitrate reductase (nitA) locus. This element, which we have named Jordan, has short (12-bp) terminal inverted repeats and creates a 3-bp target site duplication, like some higher plant transposons of the classic type. Contained within the first 200 bp of one end of the element are 55-bp inverted repeats, one of which begins with the terminal inverted repeat. Revertants of the transposon insertion into the nitA locus were obtained at a rate of . u~O -~ per Volvox embryo per generation. In each revertant examined, all transposon sequences were completely excised, but footprints containing both sets of duplicated bases, in addition to three to nine extra bases, were left behind. Jordan contains no significant open reading frames and so appean to be nonautonomous. DNA gel blot analysis indicates that Jordan is a member of a large family of homologous elements in the Volvox genome. We have isolated and characterized several of these homologs and found that they contain termini very similar to those of Jordan. Efforts to utilize Jordan and its homologs as tools to tag and clone developmentally interesting genes of Volvox are discussed.

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“…987; Knapp et al, 1988;Yoder et al, 1988). This advance offers great opportunities both for dissecting the function of transposons by reverse genetics and for extending the application of gene-tagging as a method for isolating genes, the products of which are unknown (Bingham, Levis, and Rubin, 1981;Fedoroff, Furtek, and Nelson, 1984;Martin et al, 1985;Moerman, Benian, and Waterston, 1986).…”

Section: Introductionmentioning

confidence: 99%

Transposable elements can be used to study cell lineages in transgenic plants.

et al. 1989

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The P-glucuronidase reporter gene has been used to develop a sensitive assay for the excision of transposable elements introduced into transgenic plants. The reporter gene, inactivated by the insertion of the maize transposable element Activator (Ac) into the 5'-untranslated leader, was introduced into the genome of tobacco by Agrobacteriummediated transformation. Reactivation of the 8-glucuronidase gene was detected in transgenic plants using a fluorometric or histochemical assay. Reactivation of the reporter gene was dependent on the presence of the transposase of Ac, and resulted from the excision of the Ac element. This assay, together with the improved methods for visualization, will provide a valuable and rapid method for studying the basic mechanism of transposition in plants and for developing modified transposable element

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Ac transposition in transgenic tomato plants

Cited by 169 publications

References 19 publications

A comparative study of Tam3 and Ac transposition in transgenic tobacco and petunia plants

A comparative study of Tam3 and Ac transposition in transgenic tobacco and petunia plants

Jordan, an active Volvox transposable element similar to higher plant transposons.

Transposable elements can be used to study cell lineages in transgenic plants.

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