AC Magnetic Heating of Superparamagnetic Fe and Co Nanoparticles

Lukawska, Anna; Jagoo, Zafrullah; Kozlowski, Gregory; Turgut, Z.; Kosai, Hiroyuki; Sheets, A. O.; Bixel, Tyler; Wheatley, Andrew E. H.; Abdulkin, Pavel; Knappett, Benjamin R.; Houlding, Thomas K.; Degirmenci, Volkan

doi:10.4028/www.scientific.net/ddf.336.159

Cited by 2 publications

(2 citation statements)

References 11 publications

(19 reference statements)

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“…Accordingly, siRNA cannot be readily taken up by cells and efficient delivery vehicles for siRNA are critical for the delivery of therapeutic siRNA. During the past decade, several types of nanoparticle (NP) delivery systems have been developed, including inorganic NPs, lipid-based nanocarriers, polymeric NPs, dendrimers, solid lipid NPs, and carbon NPs [ 8 , 9 ]. For GBM, siRNA targeting MGMT (O 6 -methylguanine methyltransferase, a key factor in brain tumor resistance to TMZ) delivered by iron oxide NPs conjugated with chlorotoxin could enhance temozolomide (TMZ) toxicity [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

PEI-Coated Fe3O4 Nanoparticles Enable Efficient Delivery of Therapeutic siRNA Targeting REST into Glioblastoma Cells

Wang

Degirmenci

Xin

et al. 2018

IJMS

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Glioblastomas (GBM) are the most frequent brain tumors lacking efficient treatment. The increasingly elucidated gene targets make siRNA-based gene therapy a promising anticancer approach, while an efficient delivery system is urgently needed. Here, polyethyleneimine (PEI)-coated Fe3O4 nanoparticles (NPs) have been developed and applied for siRNA delivery into GBM cells to silence repressor element 1-silencing transcription factor (REST). The prepared PEI-coated Fe3O4 NPs were characterized as magnetic nanoparticles with a positive charge, by transmission electronic microscopy, dynamic light-scattering analysis and a magnetometer. By gel retardation assay, the nanoparticles were found to form complexes with siRNA and the interaction proportion of NP to siRNA was 2.8:1. The cellular uptake of NP/siRNA complexes was verified by prussian blue staining, fluorescence labeling and flow cytometry in U-87 and U-251 GBM cells. Furthermore, the REST silencing examined by realtime polymerase chain reaction (PCR) and Western blotting presented significant reduction of REST in transcription and translation levels. Upon the treatment of NP/siRNA targeting REST, the GBM cell viabilities were inhibited and the migration capacities were repressed remarkably, analyzed by cell counting kit-8 and transwell assay separately. In this study, we demonstrated the PEI-coated Fe3O4 nanoparticle as a vehicle for therapeutic siRNA delivery, at an appropriate NP/siRNA weight ratio for REST silencing in GBM cells, inhibiting cell proliferation and migration efficiently. These might represent a novel potential treatment strategy for GBM.

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Section: Introductionmentioning

confidence: 99%

PEI-Coated Fe3O4 Nanoparticles Enable Efficient Delivery of Therapeutic siRNA Targeting REST into Glioblastoma Cells

Wang

Degirmenci

Xin

et al. 2018

IJMS

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“…Domain walls arise when magnetostatic energy and domain-wall energy are in equilibrium [ 41 , 42 ]. The domain-wall energy is responsible for the larger interfacial area between domains, and the magnetostatic energy grows proportionately with the volume of the material [ 43 ]. The energy required to create domain barriers is substantially more than the magnetostatic energy required to maintain a single-domain NP; hence, there is a critical value below which a stable single-domain NP can be established when the size of ferromagnetic materials is lowered to the nanoscale level [ 44 ].…”

Section: Types Of Magnetic and Fluorescent Nanoparticlesmentioning

confidence: 99%

Bioimaging Probes Based on Magneto-Fluorescent Nanoparticles

Ganguly

Margel

2023

Pharmaceutics

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Novel nanomaterials are of interest in biology, medicine, and imaging applications. Multimodal fluorescent-magnetic nanoparticles demand special attention because they have the potential to be employed as diagnostic and medication-delivery tools, which, in turn, might make it easier to diagnose and treat cancer, as well as a wide variety of other disorders. The most recent advancements in the development of magneto-fluorescent nanocomposites and their applications in the biomedical field are the primary focus of this review. We describe the most current developments in synthetic methodologies and methods for the fabrication of magneto-fluorescent nanocomposites. The primary applications of multimodal magneto-fluorescent nanoparticles in biomedicine, including biological imaging, cancer treatment, and drug administration, are covered in this article, and an overview of the future possibilities for these technologies is provided.

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AC Magnetic Heating of Superparamagnetic Fe and Co Nanoparticles

Cited by 2 publications

References 11 publications

PEI-Coated Fe3O4 Nanoparticles Enable Efficient Delivery of Therapeutic siRNA Targeting REST into Glioblastoma Cells

PEI-Coated Fe3O4 Nanoparticles Enable Efficient Delivery of Therapeutic siRNA Targeting REST into Glioblastoma Cells

Bioimaging Probes Based on Magneto-Fluorescent Nanoparticles

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