<p><strong>Abstract.</strong> Dissolved organic carbon concentration in the Pearl River estuary (PRE) of China was measured in May, August, and October 2015 and January 2016. Chromophoric and fluorescent dissolved organic matter (CDOM and FDOM) in the latter three seasons were characterized by absorption and fluorescence spectroscopy. Parallel factor analysis of the fluorescence spectra identified two protein-like, two humic-like, and one oxidized quinone-like FDOM components. The seasonality of average DOM abundance varied as follows: DOC: May (156&#8201;&#956;mol&#8201;L<sup>&#8722;1</sup>)&#8201;>&#8201;January (114&#8201;&#956;mol&#8201;L<sup>&#8722;1</sup>)&#8201;&#8776;&#8201;August (112&#8201;&#956;mol&#8201;L<sup>&#8722;1</sup>)&#8201;>&#8201;November (86&#8201;&#956;mol&#8201;L<sup>&#8722;1</sup>); CDOM absorption at 330&#8201;nm: August (1.76&#8201;m<sup>&#8722;1</sup>)&#8201;>&#8201;November (1.39&#8201;m<sup>&#8722;1</sup>)&#8201;&#8776;&#8201;January (1.30&#8201;m<sup>&#8722;1</sup>); FDOM expressed as the sum of the maximum fluorescence intensities of all FDOM components: November (1.77&#8201;R.U.)&#8201;>&#8201;August (1.54&#8201;R.U.)&#8201;&#8776;&#8201;January (1.49&#8201;R.U.). Average DOM abundance in surface water was higher than in bottom water, their difference being marginal (0.1&#8211;10&#8201;%) for DOC in all seasons and for CDOM and FDOM in November and January, and moderate (16&#8211;21&#8201;%) for CDOM and FDOM in August. DOC showed little cross-estuary variations in all seasons while CDOM and FDOM in January were higher on the west side of the estuary than in the middle and on the east side. All three variables exhibited large variations and/or rapid drawdowns at the head of the estuary (salinity&#8201;<&#8201;5) due to multiple freshwater endmembers and/or biotic losses. In the saltier zone, they declined linearly with salinity except relatively constant DOC in May and November. The decrease in FDOM was 5&#8211;35&#8201;% faster than that in CDOM, which in turn was 2&#8211;3 times faster than that in DOC. Salinity and CDOM absorption coefficients can serve as indicators of DOC in August and January. Absorbance- and fluorescence-based indices demonstrate that freshwater endmembers in all seasons mainly contained fresh, protein-rich DOM of microbial origin, though the proportion of humic-like components was somewhat higher in August. Protein-like materials were preferentially consumed in the low-salinity section but the dominance of the microbial signature was maintained throughout the saltier zone. Exports of DOC and CDOM (in terms of <i>a</i><sub>330</sub>) into the South China Sea were estimated as 195&#8201;&#215;&#8201;10<sup>9</sup>&#8201;g and 266&#8201;&#215;&#8201;10<sup>9</sup>&#8201;m<sup>2</sup> for the PRE, and 362&#8201;&#215;&#8201;10<sup>9</sup>&#8201;g and 493&#8201;&#215;&#8201;10<sup>9</sup>&#8201;m<sup>2</sup> for the entire Pearl River Delta. Compared to other world major estuaries, the PRE presents the lowest concentrations and export fluxes of DOC and CDOM. Nonetheless, DOM delivered by the PRE is protein-rich and thus may significantly impact the local ecosystem.</p>