2005
DOI: 10.1007/s00280-005-1004-5 View full text |Buy / Rent full text
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Abstract: Breast cancer resistance protein (BCRP/ABCG2) is an ATP-binding cassette (ABC) multidrug transporter that confers resistance to various anticancer drugs like topotecan and mitoxantrone. To obtain more insight in its cellular functioning, we investigated phosphorylation and N-linked glycosylation of BCRP. In the epithelial Madin-Darby canine kidney (MDCK) cell line, we did not detect phosphorylation of BCRP, in contrast to MRP2, which was phosphorylated. In the ovarian carcinoma cell line T8 also no phosphoryla… Show more

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“…4). The calculated directional permeabilities, for both ABC and AZT, show that, given the known orientation of Bcrp1 in the transfected MDCKII cells (Mohrmann et al, 2005), there was the expected significant increase in the B-to-A transport over the A-to-B transport in the Bcrp1-transfected cells, which was significantly higher than in the wild-type cells. This Bcrp1-mediated transport of either nucleoside was not saturated even in the presence of a high concentration (50 M ABC and AZT) of unlabeled nucleoside (data not shown).…”
Section: Discussionmentioning
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rupbmjkragerfmgwileyiopcupepmcmbcthiemesagefrontiersapsiucrarxivemeralduhksmucshluniversity-of-gavle
“…4). The calculated directional permeabilities, for both ABC and AZT, show that, given the known orientation of Bcrp1 in the transfected MDCKII cells (Mohrmann et al, 2005), there was the expected significant increase in the B-to-A transport over the A-to-B transport in the Bcrp1-transfected cells, which was significantly higher than in the wild-type cells. This Bcrp1-mediated transport of either nucleoside was not saturated even in the presence of a high concentration (50 M ABC and AZT) of unlabeled nucleoside (data not shown).…”
Section: Discussionmentioning
“…superfamily can exhibit glycosylation defects that result in altered localization [23,38]. At present, the conclusive significance of such post-translational modifications is poorly elucidated and controversial data have been reported [39,40]. for the outcome of ovarian carcinoma patients supports the relevance of the transporters in ovarian carcinoma aggressiveness [42].…”
Section: Discussionmentioning
“…In addition to changes at the protein level, the subcellular localization of ABCG2 was also altered by tunicamycin. A previous report showed that NLG is not essential for routing ABCG2 to the plasma membrane because the N596A mutation had little effect on ABCG2 subcellular localization (28). However, the study used site-specific mutations, and these genetic modifications differ significantly from tunicamycin treatment, which was able to inhibit Akt phosphorylation.…”
Section: Discussionmentioning
“…ABCG2 is an N-linked glycosylated protein localized to the apical domain of plasma membranes (28). In particular, the NLG site corresponding to Asn596 in human ABCG2 is highly conserved.…”
Section: Glycosylation Inhibitors Affected Abcg2 Protein Levelsmentioning