2013
DOI: 10.18632/aging.100615
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Abstract: Meibomian gland dysfunction (MGD) is frequent with aging and is the primary cause of dry eye disease, the most prevalent ocular complaint. We used a novel 3-D reconstruction technique, immunofluorescent computed tomography (ICT), to characterize meibomian gland keratinization and cell proliferation in a mouse model of age-related meibomian gland dysfunction (ARMGD). To visualize the changes associated with ARMGD, 5-month and 2-year old mouse eyelids were 3-D reconstructed by ICT using antibodies to cytokeratin… Show more

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Cited by 44 publications
(50 citation statements)
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References 39 publications
(50 reference statements)
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“…This finding is consistent with previous reports describing CK6 in epithelial duct cells of meibomian glands. 13,47 In this study, intensive CK6 expression increased over time. It is therefore possible to speculate that culture conditions mimicked the microenvironment around meibomian gland ducts and hence elicited differentiation toward keratinization at least in meibocytes at the air-liquid interface as already discussed for CK10.…”
Section: Discussionsupporting
confidence: 51%
“…This finding is consistent with previous reports describing CK6 in epithelial duct cells of meibomian glands. 13,47 In this study, intensive CK6 expression increased over time. It is therefore possible to speculate that culture conditions mimicked the microenvironment around meibomian gland ducts and hence elicited differentiation toward keratinization at least in meibocytes at the air-liquid interface as already discussed for CK10.…”
Section: Discussionsupporting
confidence: 51%
“…Modifications on MG structure have been identified with age as, an altered localization of the peroxisome proliferator-activated receptor-ɣ (PPAR-ɣ), a lipid-activated hormone receptor that regulates lipid synthesis and cell differentiation [16,17] or MG atrophy [18]. In research on mouse model, some authors have found changes in the mucocutaneous junction and glandular atrophy through a loss of meibocyte progenitors [18]. The aging, in fact, leads to a decrease of the acinar diameter and an increase of acinar wall inhomogeneity without significant modifications of the glandular orifice diameter, altering qualitatively the meibum secretion.…”
Section: Aging and Lacrimal Discomfortmentioning
confidence: 99%
“…142 Recent studies have shown that aged C57BL/6 mice are a valuable tool to study age-related dry eye, as these mice develop age-related MGD, corneal staining, goblet cell loss and conjunctival lymphocytic infiltration and dacryoadenitis. 23, 145, 146 Similarly to desiccated mice and human Sjögren Syndrome (SS) patients, there is an increase production of MMP-9 and T-cell related cytokines such as IL-17, IFN-γ, on the aged ocular surface and an influx of CD4 + and CD8 + T cells into the aged LG 23 , suggesting that multiple inciting mechanism may converge to produce clinically significant dry eye independent of the initiating factor. Inflammation may be a common denominator in these situations.…”
Section: Introductionmentioning
confidence: 99%
“…26 Aged C57BL/6 mice also develop age-related MGD, characterized by a dropout in acini volume, and increased expression of cytokines, and increased meibocyte differentiation. 145, 146, 172, 173 It is postulated that a decrease in acini volume maybe secondary to loss of stem cells within the meibomian gland and not necessary to duct keratinization, as no increase in Keratin 10 nor SPRR1-a was noted in aged C57BL/6 MG. 145, 173 In elegant studies using immunofluorescent computed tomography and 3D reconstruction, no duct keratinization was observed in aged meibomian glands from C57BL/6 mice, further corroborating their findings. 145 …”
Section: Introductionmentioning
confidence: 99%