Abnormal bone collagen morphology and decreased bone strength in growth hormone-deficient rats

Lange, Marc; Qvortrup, Klaus; Svendsen, Ole Lander; Flyvbjerg, Allan; Nowak, Jette; Petersen, Michael Mørk; Ølgaard, Klaus; Feldt‐Rasmussen, Ulla

doi:10.1016/j.bone.2004.02.025

Cited by 13 publications

(10 citation statements)

References 25 publications

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“…This suggests that a chronically high level of GH/IGF1, with a concomitant increase in collagen expression and in absolute collagen synthesis, correlates to smaller collagen fibril area in tendon. In the only other in vivo study correlating the level of GH to collagen fibril dimensions, bone collagen fibril diameter was found to be larger in GHD rats compared to wild-type controls (13), which is in support of the observations in the present study. GH/IGF1 could potentially affect known regulators of collagen fibril diameter/area such as proteoglycans including decorin (56,57).…”

Section: Quantitative Collagen Morphologysupporting

confidence: 92%

“…Local muscle IGF1Ea mRNA expression correlated significantly with both muscle collagen I mRNA expression (r Average tracer to tracee ratios in plasma and tissue 13 C/ 12 C proline was 0.18854 in plasma. In tissue, 13 C/ 12 C proline was 0.01091 in tendon collagen protein, 0.01090 in muscle collagen protein and 0.01090 in muscle myofibrillar protein.…”

Section: Igf1/collagen Correlationmentioning

confidence: 90%

“…It is possible that the observed association between GH/IGF1 and collagen morphology in human tendon also exists in other musculoskeletal tissues. This is interesting, given that GHD rats have significantly decreased bone strength (13), and that GHD patients suffer from a high incidence of bone fractures (14).…”

Section: Quantitative Collagen Morphologymentioning

confidence: 99%

“…In tissue, 13 C/ 12 C proline was 0.01091 in tendon collagen protein, 0.01090 in muscle collagen protein and 0.01090 in muscle myofibrillar protein. …”

Section: Igf1/collagen Correlationmentioning

confidence: 99%

“…One study in GHD rats suggested that bone collagen fibril diameter and GH levels were inversely correlated (13), which could in part explain the high incidence of bone fractures seen in GHD patients (14). However, a possible association between GH/IGF1 titre and collagen fibril diameter is unexplored in man.…”

Section: Introductionmentioning

confidence: 99%

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GH and IGF1 levels are positively associated with musculotendinous collagen expression: experiments in acromegalic and GH deficiency patients

Doessing¹,

Holm²,

Heinemeier³

et al. 2010

European Journal of Endocrinology

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Objective: Disproportionate growth of musculoskeletal tissue is a major cause of morbidity in both acromegalic (ACRO) and GH-deficient (GHD) patients. GH/IGF1 is likely to play an important role in the regulation of tendon and muscle collagen. We hypothesized that the local production of collagen is associated with the level of GH/IGF1. Design and methods: As primary outcomes, collagen mRNA expression and collagen protein fractional synthesis rate (FSR) were determined locally in skeletal muscle and tendon in nine ACRO and nine GHD patients. Moreover, muscle myofibrillar protein synthesis and tendon collagen morphology were determined. Results and conclusions: Muscle collagen I and III mRNA expression was higher in ACRO patients versus GHD patients (P!0.05), whereas collagen protein FSR did not differ significantly between ACRO and GHD patients in muscle (PZ0.21) and tendon (PZ0.15). IGF1Ea and IGF1Ec mRNA expression in muscle was higher in ACRO patients versus GHD patients (P!0.01). Muscle IGF1Ea mRNA expression correlated positively with collagen I mRNA expression (P!0.01). Tendon collagen fibrillar area tended to be higher in GHD patients relative to ACRO patients (PZ0.07). Thus, we observed a higher expression for collagen and IGF1 mRNA in local musculotendinous tissue in ACRO patients relative to GHD patients. Moreover, there was a tendency towards a higher collagen protein FSR and a smaller collagen fibril diameter in ACRO patients relative to GHD patients. The results indicate a collagenstimulating role of local IGF1 in human connective tissue and add to the understanding of musculoskeletal pathology in patients with either high or low GH/IGF1 axis activity.

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Section: Quantitative Collagen Morphologysupporting

confidence: 92%

Section: Igf1/collagen Correlationmentioning

confidence: 90%

Section: Quantitative Collagen Morphologymentioning

confidence: 99%

“…In tissue, 13 C/ 12 C proline was 0.01091 in tendon collagen protein, 0.01090 in muscle collagen protein and 0.01090 in muscle myofibrillar protein. …”

Section: Igf1/collagen Correlationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

GH and IGF1 levels are positively associated with musculotendinous collagen expression: experiments in acromegalic and GH deficiency patients

Doessing¹,

Holm²,

Heinemeier³

et al. 2010

European Journal of Endocrinology

Self Cite

View full text Add to dashboard Cite

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Hierarchically Staggered Nanostructure of Mineralized Collagen as a Bone‐Grafting Scaffold

et al. 2016

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A hierarchical, intrafibrillarly mineralized collagen (HIMC) is achieved through a selective mineralization progress in the collagenous gap regions mediated by poly(acrylic acid) with appropriate molecular weight. The associated topographical features directly correlate with nanomechanical heterogeneities of the HIMC to accommodate a broad range of external loads. Moreover, this hierarchically staggered nanostructure provides an optimized microenvironment to improve bone regeneration by instructing host cells.

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Fundamental Biomechanics in Bone Tissue Engineering

Wang

Nyman

Dong

et al. 2010

Synthesis Lectures on Tissue Engineering

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Abnormal bone collagen morphology and decreased bone strength in growth hormone-deficient rats

Cited by 13 publications

References 25 publications

GH and IGF1 levels are positively associated with musculotendinous collagen expression: experiments in acromegalic and GH deficiency patients

GH and IGF1 levels are positively associated with musculotendinous collagen expression: experiments in acromegalic and GH deficiency patients

Hierarchically Staggered Nanostructure of Mineralized Collagen as a Bone‐Grafting Scaffold

Fundamental Biomechanics in Bone Tissue Engineering

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