Abnormal antioxidant defence in some tissues of congenitally obese mice

Capel, Ifor D.; Dorrell, H. M.

doi:10.1042/bj2190041

Cited by 32 publications

(28 citation statements)

References 48 publications

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“…Capel and Dorell demonstrated that ob/ob mice compared with wild type mice had reduced levels of hepatic GPx and GSH [1]. Consistent with this observation, Park et al, showed that high-fat diet-induced obese mice also had lower hepatic GSH level [14].…”

Section: Resultssupporting

confidence: 70%

“…Mutant mice that cannot produce the appetite-suppressing hormone leptin (ob/ob mice) eat to the point of extreme obesity. These mice, as well as high-fat diet-induced obese mice, generate high amounts of ROS and demonstrate increased hepatic lipid peroxidation compared with healthy wild type mice [1,4,8,23]. As such, ob/ob mice were employed in the current study to explore the impact of Jeju ground water on the hepatic antioxidant system.…”

Section: Introductionmentioning

confidence: 99%

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Consumption of Jeju Ground Water Containing Vanadium Components Enhances Hepatic Antioxidant Defense Systems in ob/ob Mice

Kim¹,

You²,

Hyun³

2012

Journal of Life Science

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The present study examined the effects of consumption of Jeju ground water containing vanadium components on oxidative stress in obese (ob/ob) mice. Intake of Jeju ground water decreased the generation of oxidative stress induced-lipid peroxidation in the liver of ob/ob mice It also enhanced the enzymatic antioxidant defense system by increasing the protein expression and activity of superoxide dismutase, catalase, and glutathione peroxidase in liver tissues. Jeju ground water intake also upregulated the intracellular content of reduced glutathione. The induction of antioxidant enzyme expression by consumption of Jeju ground water was mediated by the erythroid transcription factor NF-E2 (Nrf2). Increased nuclear expression of phospho Nrf2 was observed in ob/ob mouse liver cells following intake of Jeju ground water. These results suggest that consumption of Jeju ground water stimulated the antioxidant defense system in the livers of ob/ob mice via induction of Nrf2.

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Section: Resultssupporting

confidence: 70%

Section: Introductionmentioning

confidence: 99%

Consumption of Jeju Ground Water Containing Vanadium Components Enhances Hepatic Antioxidant Defense Systems in ob/ob Mice

Kim¹,

You²,

Hyun³

2012

Journal of Life Science

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“…NAC was tried because reduced levels of GSH have been observed in animal models of NASH. 38,39 NAC increases the availability of intracellular GSH through the provision of cysteine for GSH biosynthesis and acting as an antioxidant molecule by itself. 40,41 In our hands, NAC both restored the intracellular pool of GSH and increased catalase-like activity in the livers of ob/ob animals to higher levels than those observed in control lean littermates.…”

Section: Discussionmentioning

confidence: 99%

Pivotal role of superoxide anion and beneficial effect of antioxidant molecules in murine steatohepatitis

et al. 2004

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Nonalcoholic fatty liver disease, frequently associated with obesity, can lead to nonalcoholic steatohepatitis (NASH) and cirrhosis. The pathophysiology of NASH is poorly understood, and no effective treatment is available. In view of a potential deleterious role for reactive oxygen species (ROS), we investigated the origin of ROS overproduction in NASH. Mitochondrial production of ROS and its alterations in the presence of antioxidant molecules were studied in livers from ob/ob mice that bear a mutation of the leptin gene and develop experimental NASH. N-acetyl-cysteine and the superoxide dismutase (SOD) mimics ambroxol, manganese [III] tetrakis (5,10,15,20 benzoic acid) (MnTBAP), and copper [II] diisopropyl salicylate (CuDIPS) were used to target different checkpoints of the oxidative cascade to determine the pathways involved in ROS production. Liver mitochondria from ob/ob mice generated more O 2°؊ than those of lean littermates (P < .01). Ex vivo, all three SOD mimics decreased O 2°؊ generation (P < .001) and totally inhibited lipid peroxidation (P < .001) versus untreated ob/ob mice. Those modifications were associated with in vivo improvements: MnTBAP and CuDIPS reduced weight (P < .02) and limited the extension of histological liver steatosis by 30% and 52%, respectively, versus untreated ob/ob mice. In conclusion, these data demonstrate deleterious effects of superoxide anions in NASH and point at the potential interest of nonpeptidyl mimics of SOD in the treatment of NASH in

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“…Similarly, liver GPX was reported to be decreased in a previous study on ob/ob mice [7,8], but the CAT activity was not determined in those studies. The CuZnSOD was unaltered in the present obese liver.…”

Section: Discussionmentioning

confidence: 95%

“…Thus, study of the oxidant-antioxidant balance in obesity is of clinical importance. However, little is known about the oxidantantioxidant status in obesity, and data are limited to the genetically obese ob/ob mouse [6][7][8][9]. Abnormal metabolism of trace metals, which is not directly linked to excessive weight gain, is reported to exist in the ob/ob mouse [10].…”

Section: Discussionmentioning

confidence: 99%

Lipid peroxidation and antioxidant enzyme status in various tissues of mice with gold-thioglucose-induced obesity.

Asayama

Hayashibe

Dobashi

et al. 1990

J. Clin. Biochem. Nutr.

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SummaryTo determine whether an oxidant-antioxidant imbalance exists in the non-genetic obese model, we induced obesity in 3-week-old female ICR mice by gold-thioglucose administration, and the lipid peroxide level and activities of antioxidant enzymes were measured in the plasma, liver, heart, and skeletal muscle. Two types of superoxide dismutase were assayed by radioimmunoassays. Lipid peroxide was estimated from fluorimetric measurement of thiobarbituric acid-reactive substances (TBARS). The plasma levels of both cholesterol (Chol) and triacylglycerol (TG) and liver TG content were increased in the obese mice. The superoxide dismutases were unaltered, while the activities of both glutathione peroxidase and catalase in the obese liver and heart were decreased. Lipid peroxide levels were unaltered in the obese liver and heart, decreased in the skeletal muscle, and increased in the plasma. The body weight correlated positively with the liver weight, heart weight, liver TG, liver Chol, plasma TG, plasma Chol, and plasma TBARS, and inversely with the tissue levels of some of the antioxidant enzymes and TBARS. The tissue levels of the antioxidant enzymes and TBARS had a general tendency to decrease in the obese animals. These results suggest that oxidative stress is enhanced only in the plasma of the obese mice and not in any of the tissues studied here. lism produces an accumulative deleterious change in mitochondrial function at a rate related to the rate of oxygen consumption, leading to the aging of the cells [1]. According to this hypothesis, food restriction decreases oxygen consumption, and, in turn, free radical production, resulting in a decreased rate of progression of degenerative disorders including lipid peroxidation [2]. We previously demonstrated that prolonged fasting suppressed mitochondrial oxidative metabolism and lipid peroxidation in certain rat tissues [3]. Conversely, increased food consumption accompanying obesity can enhance oxidative stress, thereby accelerating the degeneration process. Obesity is known to be an independent risk factor of atherosclerosis [4], and the latter process is postulated to be accelerated by an increase in the level of circulating lipid peroxides [5]. Thus, study of the oxidant-antioxidant balance in obesity is of clinical importance. However, little is known about the oxidantantioxidant status in obesity, and data are limited to the genetically obese ob/ob mouse [6][7][8][9]. Abnormal metabolism of trace metals, which is not directly linked to excessive weight gain, is reported to exist in the ob/ob mouse [10]. Such deviation in trace metal status per se may modify tissue levels of antioxidant enzymes, which contain trace metals in their active centers, independently of energy metabolism.To determine whether an oxidant-antioxidant imbalance exists in the nongenetic model of obesity, the obese condition was induced by gold-thioglucose administration to mice, and the lipid peroxide level and activities of antioxidant enzymes were measured in various tissues. MATERI...

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Abnormal antioxidant defence in some tissues of congenitally obese mice

Cited by 32 publications

References 48 publications

Consumption of Jeju Ground Water Containing Vanadium Components Enhances Hepatic Antioxidant Defense Systems in ob/ob Mice

Consumption of Jeju Ground Water Containing Vanadium Components Enhances Hepatic Antioxidant Defense Systems in ob/ob Mice

Pivotal role of superoxide anion and beneficial effect of antioxidant molecules in murine steatohepatitis

Lipid peroxidation and antioxidant enzyme status in various tissues of mice with gold-thioglucose-induced obesity.

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