Abiotic stress enhances androgenesis from isolated microspores of some legume species (Fabaceae)

Ochatt, Sergio; Pech, C.; Grewal, Ravinder K.; Conreux, Catherine; Lulsdorf, M.; Jacas, L.

doi:10.1016/j.jplph.2009.01.011

Cited by 74 publications

(70 citation statements)

References 29 publications

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“…Pisum and Lupinus species are known to be recalcitrant to in vitro culture [4][5][6][7]. In spite of numerous studies focused on the production of doubled haploids (DH), no important results have been noted to date [8][9][10][11]. For shortening the breeding cycle, the single seed descent technique (SSD) in combination with in vitro culture of immature embryos may be an alternative to the DH system.…”

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confidence: 99%

Preliminary results of in vitro culture of pea and lupin embryos for the reduction of generation cycles in single seed descent technique

et al. 2013

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Pea and lupin plants have been potentially rich sources of protein for human and animal diets. Currently, in Poland the main source of protein is soya cake, imported to Poland in amount of 2 mln tons per year. For substitution of soya with domestic protein plants breeding methods for higher and stable yield of grain legumes should be developed. About 12 generations are needed to develop a new cultivar and under central or north European climate conditions only one generation per year is feasible in the field. For that reason the development of new tools is needed to accelerate obtaining homozygosity in breeding of pea and lupins. Homozygosity can be attained by selfing successive generations or by hapoidization of hybrids using different in vitro techniques and doubling chromosomes in haploid plants (for review see, e.g., [1][2][3]). Pisum and Lupinus species are known to be recalcitrant to in vitro culture [4][5][6][7]. In spite of numerous studies focused on the production of doubled haploids (DH), no important results have been noted to date [8][9][10][11]. For shortening the breeding cycle, the single seed descent technique (SSD) in combination with in vitro culture of immature embryos may be an alternative to the DH system. Currently, SSD populations are frequently used as alternatives to DH populations in genetic and genomic studies (see, for example, [12,13]). The SSD technique connected with embryo in vitro culture allows the attainment of homozygous lines in a relatively short time. Such an approach applied to the development of winter barley SSD lines enabled the shortening of the generation cycle to ca. 4 months (including verbalization) [14]. Ochatt et al. [15] proposed the reduction of pea generation cycles based on in vitro culture of embryos and a greenhouse strategy, but no information was found on embryo culture for accelerating breeding process in lupins.The aim of the present studies was to establish in vitro conditions for the culture of pea, and narrow-leafed and yellow lupin embryos as the first step in research aimed at shortening generation cycles in the SSD technique used to accelerate the development of homozygous populations. Material and methodsMaterial for the studies consisted of two pea cultivars (Pisum sativum L.) -Cysterski and Akord, two narrow-leafed lupin cultivars (Lupinus angustifolius L.) -Kadryl and Sonet, and two yellow lupin cultivars (L. luteus L.) -Mister and Taper. Pea and lupin cultivar seeds were sown in the experimental field at Wiatrowo near Poznań. Pods from each pea and lupin cultivar were detached 21-28 days after flowering, when seeds were still-green but fully developed. Seeds taken out of pods were sterilized by successive dips into ethanol 70% (3 min) and AbstractThe aim of the studies was to establish in vitro conditions for the culture of pea and lupin embryos as the first step in the development of an in vitro assisted single seed descent technique for the attainment of homozygous populations. Materials for the study included of pea, and narrow-leafed a...

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Preliminary results of in vitro culture of pea and lupin embryos for the reduction of generation cycles in single seed descent technique

et al. 2013

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“…Ochatt et al (2009) found a combination of osmotic stress and electrostimulation of isolated microspores of some legume species to be effective for production of microcalli, and concluded that pyramiding stress agents may trigger the switch to sporophytic development of microspores in recalcitrant species. As with surface sterilization treatments, the mildest effective stress should be used to avoid deleterious effects of harsh treatments.…”

Section: Culture and Induction Of Microsporesmentioning

confidence: 99%

“…They concluded that further development of the pro-embryos was restricted by the presence of the intact microspore exines. Recently, there have been some positive initial results on isolated microspore culture of field pea (Pisum sativum L.), grasspea (Lathyrus sativus L.), and Medicago truncatula (Ochatt et al 2009). Uninucleate microspores were found to be the most responsive, and flower buds required a dark, cold pretreatment of at least 2d at 4°C.…”

Section: Legume Speciesmentioning

confidence: 99%

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Isolated microspore culture techniques and recent progress for haploid and doubled haploid plant production

Ferrie

Caswell

2010

Plant Cell Tiss Organ Cult

219

112

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“…Successful induction of androgenesis with subsequent regeneration of complete haploid plants is limited to the following legume species: Pisum sativum, Lathyrus sativus, Medicago truncatula, M. sativa and M. arborea (Zagorska et al 1997;Lanas et al 2006;Ochatt et al 2009), Glycine max (Zhao et al 1998;Moraes et al 2004), Cajanus cajan (Kaur and Bhalla 1998), Cicer arietinum ) and for only one legume tree, Swainsona formosa (Sudhersan et al 2008). For lupin, some existing reports about the induction of haploid embryogenesis show that either early stages of nuclear division, multicellular structures (MCS) or embryo-like structures (ELS) were observed (Sator et al 1983;Sator 1985;Ormerod and Caligari 1994;Bayliss et al 2004;Skrzypek et al 2008).…”

Section: Introductionmentioning

confidence: 99%

Anther culture of Lupinus angustifolius: callus formation and the development of multicellular and embryo-like structures

et al. 2011

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Androgenesis is an important technique to generate double haploid plants. Anther and microspore cultures are the methods to induce haploid embryogenesis. For culture initiation, it is necessary to select anthers with the appropriate developmental stage of microspores. For lupins, limited reports about the establishment of initial cultures for androgenesis are available. In this study, different parameters of anther culture of three genotypes of Lupinus angustifolius were investigated. For all genotypes, a considerable correlation was observed between the buds and the anthers, depending on their location in the inflorescences. Buds from the central segment of inflorescences had yellowish green anthers that contained the maximum number of microspores at uninucleate stage. Cytological investigation shows that the anthers containing these microspores were the most responsive to induction. Two types of developmental pathways were observed for microspores. In case of cold pre-treated and untreated inflorescences, microspores developed into multicellular and embryo-like structures, respectively. Effects of different factors showed significant differences among: genotypes, pre-treatment, growth regulators (GRs) and genotypes 9 GRs interaction. Among three genotypes, Emir showed the highest number of multicellular and embryo-like structures on MS medium ? 2.0 mg/l 2,4 D ? 0.5 mg/l Kinetin (Kin). For all genotypes, anthers produced calli on MS medium containing 2.0 mg/l 2,4 D ? 0.5 mg/l Kin. These calli continued their growth on regeneration medium (MS ? 2.0 mg/l BA ? 0.5 mg/l NAA) and produced roots. Taken together, these results provide a good basis for further research towards the development of haploid plants for L. angustifolius.

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Abiotic stress enhances androgenesis from isolated microspores of some legume species (Fabaceae)

Cited by 74 publications

References 29 publications

Preliminary results of in vitro culture of pea and lupin embryos for the reduction of generation cycles in single seed descent technique

Preliminary results of in vitro culture of pea and lupin embryos for the reduction of generation cycles in single seed descent technique

Isolated microspore culture techniques and recent progress for haploid and doubled haploid plant production

Anther culture of Lupinus angustifolius: callus formation and the development of multicellular and embryo-like structures

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