Ability of Lactobacillus plantarum lipoteichoic acid to inhibit Vibrio anguillarum-induced inflammation and apoptosis in silvery pomfret (Pampus argenteus) intestinal epithelial cells

Gao, Qiang; Gao, Q. X.; Min, Minghua; Zhang, Chenjie; Peng, Shi-Qing; Shi, Zhaohong

doi:10.1016/j.fsi.2016.05.013

Cited by 44 publications

(22 citation statements)

References 26 publications

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“…Although the positive effects of L. plantarum in aquaculture animals have been investigated including enhancement of the growth performance [11,14,25], and immune response [15,35,45] and inhibition of adhesion of pathogenic microorganisms [9,10]. In this study, we didn't find any significant effect of Lp on growth performance, immune parameters and RPS of catfish compared to CG.…”

Section: Discussionmentioning

confidence: 71%

“…Lactic acid bacteria (LAB) have a reputation of being "generally regarded as safe" and are widely used in aquaculture [7,8]. L. plantarum is one of the widely used LAB in aquaculture and benefits to aquatic organisms by immunostimulation, disease resistance and growth enhancement [9][10][11][12][13]. For example, Son et al [14] reported that treatment of a diet containing L. plantarum at the level of 10 8 cfu/g for 4 weeks significantly increased percent weight gain and feed efficiency, and reduced their mortality rate more than 20% after challenged with Streptococcu ssp.…”

Section: Introductionmentioning

confidence: 99%

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Effect of Dietary Supplementation of Lactobacillus Casei YYL3 and L. Plantarum YYL5 on Growth, Immune Response and Intestinal Microbiota in Channel Catfish

Hong-yu

Wang

et al. 2019

Animals

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The purpose of this study is to investigate the effect of probiotics L. casei YYL3 (Lc) and L. plantarum YYL5 (Lp) on growth performance, innate immunity, disease resistance and intestinal microbiota of channel catfish. A total of 252 catfish (67.20 ± 1.46 g) were randomly divided into 3 groups which were fed with basal diet, Lc-added (3.0 × 108 cfu/g) or Lp-added (3.0 × 108 cfu/g) diets, respectively. After 4 weeks of feeding, Lc significantly enhanced the growth and feed utilization of channel catfish compared with the control group (CG). Following that, the catfish were challenged with an intraperitoneal injection of 200 μL of the pathogenic E.ictaluri (2.0 × 106 cfu/mL), the relative percent survival of Lc and Lp were 38.28% and 12.76%, respectively. High-throughput sequencing indicated Lc and Lp reduced the alpha diversity of the intestinal microbiota in channel catfish. Lactobacillus were overwhelming in the guts during probiotics treatment, but almost vanished away after 2 weeks post-cessation of probiotics administration. Compared to CG, Lc and Lp resulted in an increased abundance of Pseudomonas and decreased amount of Aeromonas. Functional analysis revealed that Lc treatment upregulated the relative abundance of Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways including lipid metabolism, metabolism of other amino acids, metabolism of terpenoids and polyketides, xenobiotics biodegradation and metabolism, and nucleotide metabolism. Combined, our data revealed that Lc, as a feed additive at 3.0 × 108 cfu/g, could promote the growth performance, disease resistance and dramatically change the composition of intestinal microbiota of channel catfish.

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Section: Discussionmentioning

confidence: 71%

Section: Introductionmentioning

confidence: 99%

Effect of Dietary Supplementation of Lactobacillus Casei YYL3 and L. Plantarum YYL5 on Growth, Immune Response and Intestinal Microbiota in Channel Catfish

Hong-yu

Wang

et al. 2019

Animals

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“…It has been reported that LTA from S. aureus induces inflammatory responses ( Kengatharan et al, 1998 ). In contrast, L. plantarum LTA (Lp.LTA) was recently shown to regulate the immune response through inhibiting adhesion of pathogens to host cells, thereby suppressing inflammatory activity ( Gao et al, 2016 ). These conflicting effects of various LTAs are due to their structural differences among different bacterial species ( Ryu et al, 2009 ; Kang et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Lipoteichoic Acid Inhibits Staphylococcus aureus Biofilm Formation

2018

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A biofilm is an aggregate of microorganisms in which cells adhere to biological or non-biological surfaces and is responsible for various infectious diseases. Infections caused by Staphylococcus aureus, including pneumonia, endocarditis, and osteomyelitis, are often associated with colonization and biofilm formation. Although lipoteichoic acid (LTA) is involved in biofilm formation, the specific role of LTA is not clearly understood. In this study, we demonstrated that LTA released from Lactobacillus plantarum could inhibit S. aureus biofilm formation and aggregation without affecting the growth of S. aureus in various in vitro and in vivo models. L. plantarum LTA (Lp.LTA) also inhibited biofilm formation of S. aureus clinical isolates, including a methicillin-resistant strain. Remarkably, Lp.LTA not only interfered with S. aureus biofilm formation, but it also disrupted a pre-formed biofilm. Mechanism studies demonstrated that Lp.LTA inhibited expression of the ica-operon, which is responsible for the production of poly-N-acetylglucosamine, a key molecule required for S. aureus biofilm development. Lp.LTA increased the release of autoinducer-2 from S. aureus, which contributed to the inhibition of S. aureus biofilm formation. Moreover, Lp.LTA treatment enhanced susceptibility of the biofilm to various antibiotics and to macrophages. Interestingly, Lp.LTA without D-alanine moieties was not able to inhibit biofilm formation by S. aureus. In conclusion, the present study suggests that LTA can inhibit S. aureus biofilm formation, and therefore could be applied for preventing and/or treating infectious diseases caused by S. aureus biofilms.

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“…LTA is a major component of the Gram-positive cell wall, and the structural and immunomodulatory effects of LTA vary widely among species. LTA from L. plantarum has been shown to be effective against pathogens [ 31 ]. Liu et al showed a significant increase in IL-1β and IL-6 levels in the serum of mice two hours after the intraperitoneal injection of two strains of V. alginolyticus , ATCC 17749T and E0666 [ 32 ].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Lactic Acid Bacteria on the Inhibition of Vibrio parahaemolyticus Infection and Its Application to Food Systems

2018

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This study tested the effect of lactic acid bacteria (LAB) inhibition on Vibrio parahaemolyticus BCRC (Bioresource Collection and Research Center) 10806 and BCRC 12865 in a food model. MTT [3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assays indicated that Caco-2 cells were not damaged after a two-hour treatment with lactic acid bacteria (LAB) and V. parahaemolyticus. The LAB cell culture and supernatant effectively inhibited the growth of V. parahaemolyticus in a food model. ELISA (Enzyme-linked immunosorbent assay) results indicated the significant inhibition of TNF-α; IL-1β; and IL-6; but Lactobacillus plantarum PM 222 and L. plantarum LP 735 did not significantly affect IL-8 levels. Real-time polymerase chain reaction (PCR) results indicated that LAB could inhibit the mRNA expression of proinflammatory cytokines IL-8; IL-6; and TNF-α; which were induced by V. parahaemolyticus. After rat-received LAB; the expression levels of TNF-α; IL-6; and IL-8 in the serum decreased significantly. In intestinal histology; the rat that received L. plantarum PM 222 and L. plantarum LP 010 was able to alleviate the intestinal villi damage caused by V. parahaemolyticus; which also helped reduce cell apoptosis. In conclusion; our results indicate that LAB can inhibit inflammatory responses caused by V. parahaemolyticus and can effectively inhibit the growth of V. parahaemolyticus in food products.

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Ability of Lactobacillus plantarum lipoteichoic acid to inhibit Vibrio anguillarum-induced inflammation and apoptosis in silvery pomfret (Pampus argenteus) intestinal epithelial cells

Cited by 44 publications

References 26 publications

Effect of Dietary Supplementation of Lactobacillus Casei YYL3 and L. Plantarum YYL5 on Growth, Immune Response and Intestinal Microbiota in Channel Catfish

Effect of Dietary Supplementation of Lactobacillus Casei YYL3 and L. Plantarum YYL5 on Growth, Immune Response and Intestinal Microbiota in Channel Catfish

Lipoteichoic Acid Inhibits Staphylococcus aureus Biofilm Formation

Evaluation of Lactic Acid Bacteria on the Inhibition of Vibrio parahaemolyticus Infection and Its Application to Food Systems

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