Abilities of the mCP Agar Method and CRENAME Alpha Toxin-Specific Real-Time PCR Assay To Detect Clostridium perfringens Spores in Drinking Water

Maheux, Andrée F.; Bérubé, Ève; Boudreau, Dominique K.; Villéger, Romain; Cantin, Philippe; Boissinot, Maurice; Bissonnette, Luc; Bergeron, Michel G.

doi:10.1128/aem.02791-13

Cited by 16 publications

(7 citation statements)

References 36 publications

(29 reference statements)

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“…Further, recent progress in methodological developments, such as the double-tube method [ 83 ], show that C. perfringens colonies can be observed after 5 h of incubation. Other studies to support the use of C. perfringens as a reliable alternative indicator include use of qPCR methods to detect C. perfringens in drinking water and biosolids [ 84 , 85 ].…”

Section: Scientific Assessment Of Experimental Methods and Approacmentioning

confidence: 99%

U.S. Recreational Water Quality Criteria: A Vision for the Future

Fujioka

Solo‐Gabriele

Byappanahalli

et al. 2015

IJERPH

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This manuscript evaluates the U.S. Recreational Water Quality Criteria (RWQC) of 2012, based upon discussions during a conference held 11–13 March 2013, in Honolulu, Hawaii. The RWQC of 2012 did not meet expectations among the research community because key recommended studies were not completed, new data to assess risks to bathers exposed to non-point sources of fecal indicator bacteria (FIB) were not developed, and the 2012 RWQC did not show marked improvements in strategies for assessing health risks for bathers using all types of recreational waters. The development of the 2012 RWQC was limited in scope because the epidemiologic studies at beach sites were restricted to beaches with point sources of pollution and water samples were monitored for only enterococci. The vision for the future is development of effective RWQC guidelines based on epidemiologic and quantitative microbial risk assessment (QMRA) studies for sewage specific markers, as well as human enteric pathogens so that health risks for bathers at all recreational waters can be determined. The 2012 RWQC introduced a program for states and tribes to develop site-specific water quality criteria, and in theory this approach can be used to address the limitations associated with the measurements of the traditional FIB.

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Section: Scientific Assessment Of Experimental Methods and Approacmentioning

confidence: 99%

U.S. Recreational Water Quality Criteria: A Vision for the Future

Fujioka

Solo‐Gabriele

Byappanahalli

et al. 2015

IJERPH

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“…; Maheux et al. ). The PCR amplified products are detected by agarose gel electrophoresis after staining with ethidium bromide (Khan et al.…”

Section: Polymerase Chain Reactionmentioning

confidence: 98%

Recent developments in detection and enumeration of waterborne bacteria: a retrospective minireview

et al. 2016

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Waterborne diseases have emerged as global health problems and their rapid and sensitive detection in environmental water samples is of great importance. Bacterial identification and enumeration in water samples is significant as it helps to maintain safe drinking water for public consumption. Culture‐based methods are laborious, time‐consuming, and yield false‐positive results, whereas viable but nonculturable (VBNCs) microorganisms cannot be recovered. Hence, numerous methods have been developed for rapid detection and quantification of waterborne pathogenic bacteria in water. These rapid methods can be classified into nucleic acid‐based, immunology‐based, and biosensor‐based detection methods. This review summarizes the principle and current state of rapid methods for the monitoring and detection of waterborne bacterial pathogens. Rapid methods outlined are polymerase chain reaction (PCR), digital droplet PCR, real‐time PCR, multiplex PCR, DNA microarray, Next‐generation sequencing (pyrosequencing, Illumina technology and genomics), and fluorescence in situ hybridization that are categorized as nucleic acid‐based methods. Enzyme‐linked immunosorbent assay (ELISA) and immunofluorescence are classified into immunology‐based methods. Optical, electrochemical, and mass‐based biosensors are grouped into biosensor‐based methods. Overall, these methods are sensitive, specific, time‐effective, and important in prevention and diagnosis of waterborne bacterial diseases.

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“…However, the relatively long time required to obtain results (Ն24 h) and the poor prediction of the presence of more resistant pathogens (parasites and viruses) do not allow conventional methods to fully and reliably ensure the microbiological quality of the water before it is distributed (8,33). CRENAME WGA-rtPCR assay could help to overcome the limitations of culture-based methods by providing results more quickly (5 h instead of 24 h) and by leading to better correlations with the presence of pathogens (20,34,35).…”

Section: Discussionmentioning

confidence: 99%

Molecular Method for Detection of Total Coliforms in Drinking Water Samples

Maheux

Boudreau

Bisson

et al. 2014

Appl Environ Microbiol

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e This work demonstrates the ability of a bacterial concentration and recovery procedure combined with three different PCR assays targeting the lacZ, wecG, and 16S rRNA genes, respectively, to detect the presence of total coliforms in 100-ml samples of potable water (presence/absence test). PCR assays were first compared to the culture-based Colilert and MI agar methods to determine their ability to detect 147 coliform strains representing 76 species of Enterobacteriaceae encountered in fecal and environmental settings. Results showed that 86 (58.5%) and 109 (74.1%) strains yielded a positive signal with Colilert and MI agar methods, respectively, whereas the lacZ, wecG, and 16S rRNA PCR assays detected 133 (90.5%), 111 (75.5%), and 146 (99.3%) of the 147 total coliform strains tested. These assays were then assessed by testing 122 well water samples collected in the Québec City region of Canada. Results showed that 97 (79.5%) of the samples tested by culture-based methods and 95 (77.9%), 82 (67.2%), and 98 (80.3%) of samples tested using PCR-based methods contained total coliforms, respectively. Consequently, despite the high genetic variability of the total coliform group, this study demonstrated that it is possible to use molecular assays to detect total coliforms in potable water: the 16S rRNA molecular assay was shown to be as efficient as recommended culturebased methods. This assay might be used in combination with an Escherichia coli molecular assay to assess drinking water quality.

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Abilities of the mCP Agar Method and CRENAME Alpha Toxin-Specific Real-Time PCR Assay To Detect Clostridium perfringens Spores in Drinking Water

Cited by 16 publications

References 36 publications

U.S. Recreational Water Quality Criteria: A Vision for the Future

U.S. Recreational Water Quality Criteria: A Vision for the Future

Recent developments in detection and enumeration of waterborne bacteria: a retrospective minireview

Molecular Method for Detection of Total Coliforms in Drinking Water Samples

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