ABFs, a Family of ABA-responsive Element Binding Factors

Choi, Hyung-in; Hong, Jung-Hee; Ha, Jin-ok; Kang, Jung-youn; Kim, Soo Young

doi:10.1074/jbc.275.3.1723

Cited by 1,007 publications

(853 citation statements)

References 38 publications

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“…Consistently, the analysis of the available promoter sequences (accession no. NC_008400.1 and NC_003074.4) upstream the coding regions of Arabidopsis and rice orthologs of ALDH22A1, respectively, predicted that both the putative cis-elements comprise multiple ABA-responsive elements (Marcotte et al 1989;Choi et al 2000;Simpson et al 2003;Kaplan et al 2006), by using PLACE software(http://www.dna.affrc.go.jp/ PLACE/) (Higo et al 1999). This implies that the promoter of ZmALDH22A1 probably contains ABAresponsive elements that are able to sense the signaling molecules induced by the application of exogenous ABA.…”

Section: Discussionmentioning

confidence: 99%

Significant improvement of stress tolerance in tobacco plants by overexpressing a stress-responsive aldehyde dehydrogenase gene from maize (Zea mays)

Huang

Wang

et al. 2008

Plant Mol Biol

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Aldehyde dehydrogenases (ALDHs) play a central role in detoxification processes of aldehydes generated in plants when exposed to the stressed conditions. In order to identify genes required for the stresses responses in the grass crop Zea mays, an ALDH (ZmALDH22A1) gene was isolated and characterized. ZmALDH22A1 belongs to the family ALDH22 that is currently known only in plants. The ZmALDH22A1 encodes a protein of 593 amino acids that shares high identity with the orthologs from Saccharum officinarum (95%), Oryza sativa (89%), Triticum aestivum (87%) and Arabidopsis thaliana (77%), respectively. Real-time PCR analysis indicates that ZmALDH22A1 is expressed differentially in different tissues. Various elevated levels of ZmALDH22A1 expression have been detected when the seedling roots exposed to abiotic stresses including dehydration, high salinity and abscisic acid (ABA). Tomato stable transformation of construct expressing the ZmALDH22A1 signal peptide fused with yellow fluorescent protein (YFP) driven by the CaMV35S-promoter reveals that the fusion protein is targeted to plastid. Transgenic tobacco plants overexpressing ZmALDH22A1 shows elevated stresses tolerance. Stresses tolerance in transgenic plants is accompanied by a reduction of malondialdehyde (MDA) derived from cellular lipid peroxidation.

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Section: Discussionmentioning

confidence: 99%

Significant improvement of stress tolerance in tobacco plants by overexpressing a stress-responsive aldehyde dehydrogenase gene from maize (Zea mays)

Huang

Wang

et al. 2008

Plant Mol Biol

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“…ABREs bind basic Leu zipper-type proteins known as ABRE binding factors, which are induced under stress conditions (Choi et al, 2000;Uno et al, 2000). Significantly, the VvHT5 promoter region also appears to lack the SUREs found in the promoter regions of VvHT1 and VvcwINV, which are also induced in response to powdery mildew ( Fig.…”

Section: Identification Of Aba Regulatory Motifs In the Vvht5 Promotermentioning

confidence: 99%

“…Two kilobases of sequence upstream of the predicted start codon of each of the VvHT1 to -5 and VvcwINV genes was obtained from the published grapevine PN40024 genome sequence (Jaillon et al, 2007) and analyzed for putative regulatory motifs associated with stress-induced transcription using the PLACE database (Higo et al, 1999). A number of motifs were identified: GCC box (Brown et al, 2003), MYC recognition (Abe et al, 1997), T/G box (Boter et al, 2004), wound response element (Palm et al, 1990), and abscisic acid response elements (ABREs; Choi et al, 2000;Hattori et al, 2002). Figure 3 also shows the presence of Suc-responsive motifs (SURE1, Suc box 3) shown previously by Cakir et al (2003) to be involved in the transcriptional regulation of VvHT1.…”

Section: Identification Of Aba Regulatory Motifs In the Vvht5 Promotermentioning

confidence: 99%

Involvement of Abscisic Acid in the Coordinated Regulation of a Stress-Inducible Hexose Transporter (VvHT5) and a Cell Wall Invertase in Grapevine in Response to Biotrophic Fungal Infection

2010

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Biotrophic fungal and oomycete pathogens alter carbohydrate metabolism in infected host tissues. Symptoms such as elevated soluble carbohydrate concentrations and increased invertase activity suggest that a pathogen-induced carbohydrate sink is established. To identify pathogen-induced regulators of carbohydrate sink strength, quantitative real-time polymerase chain reaction was used to measure transcript levels of invertase and hexose transporter genes in biotrophic pathogen-infected grapevine (Vitis vinifera) leaves. The hexose transporter VvHT5 was highly induced in coordination with the cell wall invertase gene VvcwINV by powdery and downy mildew infection. However, similar responses were also observed in response to wounding, suggesting that this is a generalized response to stress. Analysis of the VvHT5 promoter region indicated the presence of multiple abscisic acid (ABA) response elements, suggesting a role for ABA in the transition from source to sink under stress conditions. ABA treatment of grape leaves was found to reproduce the same gene-specific transcriptional changes as observed under biotic and abiotic stress conditions. Furthermore, the key regulatory ABA biosynthetic gene, VvNCED1, was activated under these same stress conditions. VvHT5 promoter::b-glucuronidase-directed expression in transgenic Arabidopsis (Arabidopsis thaliana) was activated by infection with powdery mildew and by ABA treatment, and the expression was closely associated with vascular tissue adjacent to infected regions. Unlike VvHT1 and VvHT3, which appear to be predominantly involved in hexose transport in developing leaves and berries, VvHT5 appears to have a specific role in enhancing sink strength under stress conditions, and this is controlled through ABA. Our data suggest a central role for ABA in the regulation of VvcwINV and VvHT5 expression during the transition from source to sink in response to infection by biotrophic pathogens.

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“…A more significant enrichment was found of the EveningElement (P < 10 -6 ) and DREB1A/CBF3 (P < 10 -4 ) and several other elements, such as the DRE core motif (P < 10 -4 ) (consensus sequence RCCGAC) (Chen et al 2002), which is a relaxed form of DREB1A/CBF3, the CBF1 binding site (BS) (P < 10 -3 ) (consensus sequence TGGCCGAC) (Hao et al 2002) and the Zbox motif (P < 10 -3 ) (consensus sequence ATACGTGT) (Ha and An 1988). Although many CS genes show responsiveness toward ABA, the ABA responsive element ABRE (consensus sequence YACGTGGC) (Choi et al 2000) was not overrepresented and only found once in the promoter of CS-20/AtERF#109. However, nine CS genes contain the ABRElike binding site motif (consensus sequence BACGTGKM) (Shinozaki and Yamaguchi-Shinozaki 2000).…”

Section: In Silico Promoter Analysis Of Cs Genesmentioning

confidence: 99%

Disease-Specific Expression of Host Genes During Downy Mildew Infection of Arabidopsis

Huibers¹,

Jong²,

Dekter³

et al. 2009

MPMI

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Here, we report on the identification of Arabidopsis genes that are induced during compatible but not during incompatible interactions with the downy mildew pathogen Hyaloperonospora arabidopsidis. This set of so-called compatible specific (CS) genes contrasts with the large group of defense-associated genes that is differentially expressed during both compatible and incompatible interactions. From the 17 identified CS genes, 6 belong to the ethylene response factor (ERF) family of transcription factor genes, suggesting that these ERF have a role during compatibility. The majority of CS genes are differentially regulated in response to various forms of abiotic stress. In silico analysis of the CS genes revealed an over-representation of dehydration-responsive element/C-repeat binding factor (DREB1A/ CBF3) binding sites and EveningElement motifs in their promoter regions. The CS-ERF are closely related to the CBF transcription factors and could potentially bind the DREB1A/CBF3 promoter elements in the CS genes. Transcript levels of CS genes peak at 2 to 3 days postinoculation, when pathogen growth is highest, and decline at later stages of infection. The induction of several CS genes was found to be isolate specific. This suggests that the identified CS genes could be the direct or indirect targets of downy mildew effector proteins that promote disease susceptibility.

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ABFs, a Family of ABA-responsive Element Binding Factors

Cited by 1,007 publications

References 38 publications

Significant improvement of stress tolerance in tobacco plants by overexpressing a stress-responsive aldehyde dehydrogenase gene from maize (Zea mays)

Significant improvement of stress tolerance in tobacco plants by overexpressing a stress-responsive aldehyde dehydrogenase gene from maize (Zea mays)

Involvement of Abscisic Acid in the Coordinated Regulation of a Stress-Inducible Hexose Transporter (VvHT5) and a Cell Wall Invertase in Grapevine in Response to Biotrophic Fungal Infection

Disease-Specific Expression of Host Genes During Downy Mildew Infection of Arabidopsis

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