ABC Spotlight on magnetic composite nanoparticles in analysis: increased sensitivity at decreased analysis time

Lieberzeit, Peter A.

doi:10.1007/s00216-018-1409-8

Cited by 3 publications

(2 citation statements)

References 14 publications

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“…coli by fluorescence measurements . Apparently, the sensitivity of these proposed methods was still not satisfactory . It is known that the monoclonal antibody is often used as a specific recognition probe; however, it is expensive and unstable under harsh conditions.…”

Section: Introductionmentioning

confidence: 99%

“…24 Apparently, the sensitivity of these proposed methods was still not satisfactory. 25 It is known that the monoclonal antibody is often used as a specific recognition probe; however, it is expensive and unstable under harsh conditions. Therefore, it is highly desirable to develop nanozymes in sensitive signal amplification, with merits such as simple preparation and costeffectiveness, 26 and to explore the antibody substitute which is stable and specific to pathogens, making great sense in developing approaches for a variety of pathogenic bacteria rapidly, selectively, and sensitively.…”

Section: Introductionmentioning

confidence: 99%

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Colorimetric Assay of Bacterial Pathogens Based on Co₃O₄ Magnetic Nanozymes Conjugated with Specific Fusion Phage Proteins and Magnetophoretic Chromatography

Liu

Han

et al. 2020

ACS Appl. Mater. Interfaces

106

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It is important to detect pathogens rapidly, sensitively, and selectively for clinical medicine, homeland security, food safety, and environmental control. We report here a specific and sensitive colorimetric assay that incorporated a bovine serum albumin-templated Co3O4 magnetic nanozyme (Co3O4 MNE) with a novel specific fusion phage protein and magnetophoretic chromatography to detect Staphylococcus aureus. The Co3O4 MNE was conjugated to S. aureus-specific fusion-pVIII (Co3O4 MNE@fusion-pVIII), screened from the S. aureus-specific phage AQTFLGEQD (the phage monoclone is denoted by the peptide sequence). The as-prepared triple-functional Co3O4 MNE@fusion-pVIII particles were capable of capturing S. aureus in sterile milk, which were then isolated from milk magnetically. Assisted by polyethylene glycol, the Co3O4 MNE@fusion-pVIII@S. aureus complex was separated from the free Co3O4 MNE@fusion-pVIII by magnetophoretic chromatography in an external magnetic field. After transferring the isolated Co3O4 MNE@fusion-pVIII@S. aureus complexes into a 96-well plate, diammonium salt of 2,2′-azino-bis(3-ethylbenzo-thiazoline-6-sulfonic acid) and H2O2 were added to develop color because of the peroxidase mimetics activity of the Co3O4 MNE. A S. aureus concentration within 10–10,000 cfu/mL in milk can be detected (detection limit: 8 cfu/mL). The as-developed method is simple, cost-efficient, and sensitive, which is useful for rapidly diagnosing pathogenic bacteria and helpful to prevent disease outbreaks induced by pathogens in developing countries.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Colorimetric Assay of Bacterial Pathogens Based on Co₃O₄ Magnetic Nanozymes Conjugated with Specific Fusion Phage Proteins and Magnetophoretic Chromatography

Liu

Han

et al. 2020

ACS Appl. Mater. Interfaces

106

View full text Add to dashboard Cite

show abstract

A simple and selective electrochemical magneto-assay for sea lice eDNA detection developed with a Quality by Design approach

Bettazzi

Orlandini

Zhang

et al. 2021

Science of The Total Environment

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Direct assessment of very-low-density lipoprotein by mass sensitive sensor with molecularly imprinted polymers

Chunta

Boonsriwong

Wattanasin

et al. 2021

Talanta

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ABC Spotlight on magnetic composite nanoparticles in analysis: increased sensitivity at decreased analysis time

Cited by 3 publications

References 14 publications

Colorimetric Assay of Bacterial Pathogens Based on Co₃O₄ Magnetic Nanozymes Conjugated with Specific Fusion Phage Proteins and Magnetophoretic Chromatography

Colorimetric Assay of Bacterial Pathogens Based on Co₃O₄ Magnetic Nanozymes Conjugated with Specific Fusion Phage Proteins and Magnetophoretic Chromatography

A simple and selective electrochemical magneto-assay for sea lice eDNA detection developed with a Quality by Design approach

Direct assessment of very-low-density lipoprotein by mass sensitive sensor with molecularly imprinted polymers

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ABC Spotlight on magnetic composite nanoparticles in analysis: increased sensitivity at decreased analysis time

Cited by 3 publications

References 14 publications

Colorimetric Assay of Bacterial Pathogens Based on Co3O4 Magnetic Nanozymes Conjugated with Specific Fusion Phage Proteins and Magnetophoretic Chromatography

Colorimetric Assay of Bacterial Pathogens Based on Co3O4 Magnetic Nanozymes Conjugated with Specific Fusion Phage Proteins and Magnetophoretic Chromatography

A simple and selective electrochemical magneto-assay for sea lice eDNA detection developed with a Quality by Design approach

Direct assessment of very-low-density lipoprotein by mass sensitive sensor with molecularly imprinted polymers

Contact Info

Product

Resources

About

Colorimetric Assay of Bacterial Pathogens Based on Co₃O₄ Magnetic Nanozymes Conjugated with Specific Fusion Phage Proteins and Magnetophoretic Chromatography

Colorimetric Assay of Bacterial Pathogens Based on Co₃O₄ Magnetic Nanozymes Conjugated with Specific Fusion Phage Proteins and Magnetophoretic Chromatography