A zebrafish vasa morphant abolishes Vasa protein but does not affect the establishment of the germline

Braat, Arie Koen; Water, Sandra van de; Korving, Jeroen; Živković, Danica

doi:10.1002/gene.1060

Cited by 47 publications

(35 citation statements)

References 15 publications

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“…However, we did not see multiplicated ventral fin folds in any of the morphants, although this is a sensitive indicator of increased BMP signaling and is a consistent feature of the ventralized din and ogon mutants . We did observe narrowing of the anterior nervous system, but morpholinos can induce nonspecific toxic effects in zebrafish (Heasman, 2002), including widespread cell death and neural degeneration (Braat et al, 2001;Lele et al, 2001). In support of this possibility, increased apoptosis occurs in the brains of tsg1 morphants (Little and Mullins, 2004) and is not seen in ventralized din mutants .…”

Section: Endogenous Tsg Enhances Bmp Signaling Through Chordin Dependsupporting

confidence: 62%

Twisted gastrulation enhances BMP signaling through chordin dependent and independent mechanisms

Xie

Fisher

2005

Development

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cleavage is crucial in restricting Chordin function in vivo, and is carried out by redundant enzymes in the zebrafish gastrula. We also present evidence that partially cleaved Chordin is a stronger BMP inhibitor than the full-length protein, suggesting a positive role for Tld in regulating Chordin. We find that depletion of the embryo for Tsg leads to decreased BMP signaling, and to increased levels of Chordin. Finally, we show that Tsg also enhances BMP signaling in the absence of Chordin, and its depletion can partially rescue the chordin mutant phenotype, demonstrating that important components of the BMP signaling pathway remain unidentified.

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Section: Endogenous Tsg Enhances Bmp Signaling Through Chordin Dependsupporting

confidence: 62%

Twisted gastrulation enhances BMP signaling through chordin dependent and independent mechanisms

Xie

Fisher

2005

Development

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“…In medaka, dnd RNA utilizes particle partition as a mechanism for asymmetric segregation of PGC fate decision during early embryogenesis (Hong et al, 2016). However, knockdown of vasa did not affect PGC survival in zebrafish and medaka (Braat et al, 2001;. Based on our present study and previous reports in gibel carp, we have identified three germ cell markers vasa, dazl and dnd.…”

Section: Discussionsupporting

confidence: 65%

Molecular characterization and expression pattern of a germ cell marker gene dnd in gibel carp (Carassius gibelio)

Liu

et al. 2016

Gene

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“…Testes require at least two weeks to develop, and oocytes can mature over several generations, therefore our treatment might not be long enough to assess the potential role of vasa in germline development. Conversely, vasa knockdown in different species shows disparate phenotypes, ranging from no defects [zebrafish (Braat et al, 2001) and flatworms (Pfister et al, 2008)] to sex-specific defects on male gamete formation, but not female [mice (Tanaka et al, 2000)], to defects in migration but not in the identity of the germ cell precursors [medaka (Li et al, 2009)]. Thus, it would not be surprising if vasa was shown not to be required for germline formation; however, its expression can still be used to identify precursors, as has been shown in other animals.…”

Section: Effects Of Vasa Knockdown In Asexual Developmentmentioning

confidence: 99%

Early lineage specification of long-lived germline precursors in the colonial ascidianBotryllus schlosseri

et al. 2009

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DEVELOPMENT 3486bodies undergo apoptosis and are removed via phagocytic cells in the blood, the primary bud migrates into the newly vacated region of the colony, opening its siphons and becoming a zooid, the secondary bud becomes the primary bud, and a new secondary bud begins to develop. Thus, the life history of Botryllus consists of a constant succession of individual zooids, each with a three-week lifespan (Lauzon et al., 2002).Asexual development (blastogenesis) takes 14 days under laboratory conditions, and can be divided into seven distinct visual stages ( Fig. 1; stages A-1 through D) (Lauzon et al., 2002). A new generation starts as a secondary bud, first visible as a thickening of the peribranchial epithelium of a primary bud ( Fig. 1; stage A1), which evaginates and forms a closed vesicle ( Fig. 1; stage A-2 through B-2). Next, a series of epithelial invaginations and protrusions ( Fig. 1; stage C-1) differentiate into somatic tissues and organs ( Fig. 1; stage C-2). After seven days ( Fig. 1; stage D), the secondary bud transitions to a primary bud and continues to develop. At day 14, the siphons open and the primary bud becomes a filterfeeding adult zooid. Each zooid can generate multiple buds each week, so the colony will eventually expand asexually. While interconnected, the zooids and buds develop independently, and individuals can be separated from the colony without disturbing their growth (i.e. subcloning), thus multiple experiments can be done on a single genotype.Following metamorphosis, colonies undergo at least 8-12 developmental cycles prior to the first appearance of gametes (sexual maturity). In addition, populations show seasonal fertility, and in the lab cycle in and out of reproductive (fertile) and nonreproductive (infertile) states. However when the colony is fertile development of the gametes is synchronized with somatic development (Mukai, 1977;Mukai and Watanabe, 1976;Sabbadin and Zaniolo, 1979). The first appearance of gonads occurs in the secondary bud (stage B), when mobile progenitors in the blood migrate to a region between the inner epithelium and the epidermis and begin to proliferate. Concurrently, oocytes at various stages of development also appear (Fig. 1). Over the next 10 days, the medial region of the blastema will differentiate into the lobular testis, while the lateral region will become the ovary (Sabbadin and Zaniolo, 1979). For the latter, one or several oocytes will become fixed on the epithelia of the peribranchial chamber, and an oviduct will form from the outer follicular layer. Upon transition to the adult zooid, mature eggs will immediately ovulate into the peribranchial chamber, be fertilized by exogenous sperm, and develop in situ. Several hours to days later the testes will complete development, and sperm will be released into the peribranchial chamber and flushed into the water column, fertilizing neighboring colonies (Johnson and Yund, 2004). The time lag between ovulation and sperm release (protogyny) prevents self-fertilization.Given this plastici...

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A zebrafish vasa morphant abolishes Vasa protein but does not affect the establishment of the germline

Cited by 47 publications

References 15 publications

Twisted gastrulation enhances BMP signaling through chordin dependent and independent mechanisms

Twisted gastrulation enhances BMP signaling through chordin dependent and independent mechanisms

Molecular characterization and expression pattern of a germ cell marker gene dnd in gibel carp (Carassius gibelio)

Early lineage specification of long-lived germline precursors in the colonial ascidianBotryllus schlosseri

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