A Versatile Star PEG Grafting Method for the Generation of Nonfouling and Nonthrombogenic Surfaces

Thalla, P.; Contreras-García, Angel; Fadlallah, Hicham; Barrette, Jérémie; Crescenzo, Grégory De; Merhi, Yahye; Lerouge, Sophie

doi:10.1155/2013/962376

Cited by 18 publications

(21 citation statements)

References 44 publications

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“…54 Our results are consistent with the literature since MNP@PO-PEG, the one coating exhibiting a lower charge than the others in analysis, needed a 48-fold longer incubation time in order to reach similar intracellular iron intake. This result is also in accordance with other studies that define PEG as one of the best non-fouling agents, [55][56][57][58] and that it also helps the nanoparticles to avoid immunological recognition of the mononuclear phagocyte system, reducing its clearance. Indeed, being a highly hydrophilic polymer, PEG forms a hydration layer leading to steric repulsion.…”

Section: Discussionsupporting

confidence: 92%

Impact of magnetic nanoparticle surface coating on their long-term intracellular biodegradation in stem cells

et al. 2019

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Magnetic nanoparticles (MNP) internalized within stem cells have paved the way for remote magnetic cell manipulation and imaging in regenerative medicine. A full understanding of their interactions with stem cells and of their fate in the intracellular environment is then required, in particular with respect to their surface coatings. Here, we investigated biological interactions of MNP composed of an identical magnetic core but coated with different molecules: phosphonoacetic acid, polyethylene glycol phosphonic carboxylic acid, caffeic acid, citric acid, and polyacrylic acid. These coatings vary in the nature of the chelating function, the number of binding sites, and the presence or absence of a polymer. The nanoparticles magnetism was systematically used as an indicator of their internalization within human stem cells and of their structural long-term biodegradation in a 3D stem cell spheroid model. Overall, we evidence that the coating impacts the aggregation status of the nanoparticles and subsequently their uptake within stem cells, but has little effect on their intracellular degradation. Only a high number of chelating functions (polyacrylic acid) had a significant protective effect. Interestingly, when the nanoparticles aggregated prior to cellular internalization, a lower degradation was also demonstrated. Finally, for all coatings, a robust dose-dependent intracellular degradation rate was demonstrated, with higher doses of internalized nanoparticles leading to lower degradation extent.

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Section: Discussionsupporting

confidence: 92%

Impact of magnetic nanoparticle surface coating on their long-term intracellular biodegradation in stem cells

et al. 2019

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“…Plasma polymer films (PPFs) are widely studied owing to their numerous potential applications mainly in the biomedical field . An important class of PPFs is the nitrogen based films which are used in, for example, influencing the differentiation of mesenchymal stem cells, enhancing the endothelialisation and healing around vascular grafts, serving as cardiovascular stent coatings and creating non‐fouling and antibacterial surfaces, where amine groups on the coating provide anchoring sites to polyethylene glycol and polyvinyl sulphonate coated silver nanoparticles, respectively. N‐rich PPFs comprise of different N functional groups such as amines, nitriles and imines.…”

Section: Introductionmentioning

confidence: 99%

Single source precursor vs. precursor mixture for N-rich plasma polymer deposition: Plasma diagnostics and thin film analyses

Buddhadasa

Vandenabeele

Snyders

et al. 2017

Plasma Process Polym

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Two distinct methods of producing N‐rich films, that mainly differ in their choice of precursor are compared; the first method involves a single source precursor and the second, a mixture of precursors that consists of a heteroatom source gas and hydrocarbon (HC) gas. Plasma diagnostics and thin film chemistry of allylamine (AA), cyclopropylamine (CPA), ammonia/ethylene (AmEt) and ammonia/1,3‐butadiene (AmBu) are studied, while maintaining the same N to C ratio, 1 to 3, in the gas phase. Single source precursors produce films containing higher N, whereas, precursor mixtures, owing to reduced dehydrogenation, produce films with a low nitrile content. While similarities between fragmentation patterns of single source precursors and precursor mixtures are observed in the plasma phase as the energy supplied is increased, chemical differences still exist between films produced by these two methods, within the studied range of power.

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“…Indeed, the physicochemical characterization confirmed the presence of a polymeric coating on all treated samples. For instance, the water contact angles that were measured for PEGylated samples were statistically different than for plain and plasma-activated titanium controls (Figure 1a) and they were in the range of wettability previously found for other PEG coatings [51,52]. The fact that no statistically significant differences were found in the average roughness (R a ) values of titanium and the PEG coated samples indicated that the coatings did not modify the morphology of the samples.…”

Section: Discussionmentioning

confidence: 67%

Polyethylene Glycol Pulsed Electrodeposition for the Development of Antifouling Coatings on Titanium

et al. 2020

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Titanium dental implants are widely used for the replacement of damaged teeth. However, bacterial infections at the interface between soft tissues and the implant can impair the functionality of the device and lead to failure. In this work, the preparation of an antifouling coating of polyethylene glycol (PEG) on titanium by pulsed electrodeposition was investigated in order to reduce Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) adhesion while maintaining human fibroblast adhesion. Different pulsed conditions were prepared and characterized by contact angle, Focused Ion Beam (FIB), Fourier Transformed Infrared Spectroscopy in the Attenuated Total Reflectance mode (ATR-FTIR), and X-ray photoelectron spectroscopy (XPS). XPS tested fibronectin adsorption. S. aureus, E. coli and human fibroblast adhesion was tested in vitro in both mono and co-culture settings. Physicochemical characterization proved useful for confirming the presence of PEG and evaluating the efficiency of the coating methods. Fibronectin adsorption decreased for all of the conditions, but an adsorption of 20% when compared to titanium was maintained, which supported fibroblast adhesion on the surfaces. In contrast, S. aureus and E. coli attachment on coated surfaces decreased up to 90% vs. control titanium. Co-culture studies with the two bacterial strains and human fibroblasts showed the efficacy of the coatings to allow for eukaryotic cell adhesion, even in the presence of pre-adhered bacteria.

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A Versatile Star PEG Grafting Method for the Generation of Nonfouling and Nonthrombogenic Surfaces

Cited by 18 publications

References 44 publications

Impact of magnetic nanoparticle surface coating on their long-term intracellular biodegradation in stem cells

Impact of magnetic nanoparticle surface coating on their long-term intracellular biodegradation in stem cells

Single source precursor vs. precursor mixture for N-rich plasma polymer deposition: Plasma diagnostics and thin film analyses

Polyethylene Glycol Pulsed Electrodeposition for the Development of Antifouling Coatings on Titanium

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