A Vacuolar Arsenite Transporter Necessary for Arsenic Tolerance in the Arsenic Hyperaccumulating Fern <i>Pteris vittata</i> Is Missing in Flowering Plants

Indriolo, Emily; Na, GunNam; Ellis, Danielle R.; Salt, David E.; Banks, Jo Ann

doi:10.1105/tpc.109.069773

Cited by 247 publications

(164 citation statements)

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“…Until this study, only fern gametophytes have been targeted for transient transformation (Rutherford et al, 2004;Indriolo et al, 2010). From our results, it is possible that choosing spores as transformation targets probably played a crucial role in successful transformation of these two fern species, which is the same case in fungi (Michielse et al, 2005;Utermark and Karlovsky, 2008), where spores have been used as targets.…”

Section: Stable Gus Expression In Ferns Transformed By a Tumefaciensmentioning

confidence: 72%

“…Transient transformation methods have been developed both in Ceratopteris richardii 'C-fern' and P. vittata, which were limited to heterologous expression in gametophytes (Rutherford et al, 2004;Indriolo et al, 2010). While transient expression of transgenic constructs does enable research, there is no substitute for stable transformation in functional genomics and plant biology.…”

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confidence: 99%

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Stable Transformation of Ferns Using Spores as Targets: Pteris vittata and Ceratopteris thalictroides

et al. 2013

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Section: Stable Gus Expression In Ferns Transformed By a Tumefaciensmentioning

confidence: 72%

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confidence: 99%

Stable Transformation of Ferns Using Spores as Targets: Pteris vittata and Ceratopteris thalictroides

et al. 2013

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“…Gametophytes were bombarded with a 35S::DsRed2 plasmid (described in ref. 34) or cobombarded with the 35S::DsRed2 plasmid plus the hairpin-forming GAIA1 mRNA plasmid (called 35S::hpGAIA1). 35S::hpGAIA1 was made by amplifying two 300-bp PCR GAIA1 fragments from cDNA, using primer pairs listed in SI Appendix, Dataset, and by amplifying the Ricinus communis Catalase Intron 1 from the 35S:irint (19).…”

Section: Methodsmentioning

confidence: 99%

Abscisic acid controlled sex before transpiration in vascular plants

McAdam

Brodribb

Banks

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

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Sexual reproduction in animals and plants shares common elements, including sperm and egg production, but unlike animals, little is known about the regulatory pathways that determine the sex of plants. Here we use mutants and gene silencing in a fern species to identify a core regulatory mechanism in plant sexual differentiation. A key player in fern sex differentiation is the phytohormone abscisic acid (ABA), which regulates the sex ratio of male to hermaphrodite tissues during the reproductive cycle. Our analysis shows that in the fern Ceratopteris richardii, a gene homologous to core ABA transduction genes in flowering plants [SNF1-related kinase2s (SnRK2s)] is primarily responsible for the hormonal control of sex determination. Furthermore, we provide evidence that this ABA-SnRK2 signaling pathway has transitioned from determining the sex of ferns to controlling seed dormancy in the earliest seed plants before being co-opted to control transpiration and CO 2 exchange in derived seed plants. By tracing the evolutionary history of this ABA signaling pathway from plant reproduction through to its role in the global regulation of plant-atmosphere gas exchange during the last 450 million years, we highlight the extraordinary effect of the ABA-SnRK2 signaling pathway in plant evolution and vegetation function.OST1 | fern | stomata | evolution | sex determination

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“…The As hyperaccumulating Pteris species differ from nonhyperaccumulating plants because of enhanced As(V) uptake (Wang et al, 2002;Poynton et al, 2004), little As(III)-thiol complexation (Zhao et al, 2003;Raab et al, 2004), and efficient xylem loading of As(III) . Recently, an As(III) efflux transporter, PvACR3, has been found to play an important role in As(III) detoxification by transporting As(III) into vacuoles in Pteris vittata (Indriolo et al, 2010).…”

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Arsenic Speciation in Phloem and Xylem Exudates of Castor Bean

et al. 2010

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How arsenic (As) is transported in phloem remains unknown. To help answer this question, we quantified the chemical species of As in phloem and xylem exudates of castor bean (Ricinus communis) exposed to arsenate [As(V)], arsenite [As(III)], monomethylarsonic acid [MMA(V)], or dimethylarsinic acid. In the As(V)-and As(III)-exposed plants, As(V) was the main species in xylem exudate (55%-83%) whereas As(III) predominated in phloem exudate (70%-94%). The ratio of As concentrations in phloem to xylem exudate varied from 0.7 to 3.9. Analyses of phloem exudate using high-resolution inductively coupled plasma-mass spectrometry and accurate mass electrospray mass spectrometry coupled to high-performance liquid chromatography identified high concentrations of reduced and oxidized glutathione and some oxidized phytochelatin, but no As(III)-thiol complexes. It is thought that As(III)-thiol complexes would not be stable in the alkaline conditions of phloem sap. Small concentrations of oxidized glutathione and oxidized phytochelatin were found in xylem exudate, where there was also no evidence of As(III)-thiol complexes. MMA(V) was partially reduced to MMA(III) in roots, but only MMA(V) was found in xylem and phloem exudate. Despite the smallest uptake among the four As species supplied to plants, dimethylarsinic acid was most efficiently transported in both xylem and phloem, and its phloem concentration was 3.2 times that in xylem. Our results show that free inorganic As, mainly As(III), was transported in the phloem of castor bean exposed to either As(V) or As(III), and that methylated As species were more mobile than inorganic As in the phloem.Arsenic (As) is an environmental and food chain contaminant that has attracted much attention in recent years. Soil contamination with As may lead to phytotoxicity and reduced crop yield (Panaullah et al., 2009). Food crops are also an important source of inorganic As, a class-one carcinogen, in human dietary intake, and there is a need to decrease the exposure to this toxin (European Food Safety Authority, 2009). Paddy rice (Oryza sativa) is particularly efficient in As accumulation, which poses a potential risk to the population based on a rice diet Zhao et al., 2010a). Other terrestrial food crops generally do not accumulate as much As as paddy rice; however, where soils are contaminated, relatively high concentrations of As in wheat (Triticum aestivum) grain have been reported Zhao et al., 2010b). On the other hand, some fern species in the Pteridaceae family are able to tolerate and hyperaccumulate As in the aboveground part to .1,000 mg kg 21 dry weight (e.g. Ma et al., 2001;Zhao et al., 2002); these plants offer the possibility for remediation of Ascontaminated soil or water (Salido et al., 2003;Huang et al., 2004). A better understanding of As uptake and long-distance transport, metabolism, and detoxification is needed for developing strategies for mitigating As contamination, through either decreased As accumulation in food crops or enhanced As accumulation for phytoremedi...

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A Vacuolar Arsenite Transporter Necessary for Arsenic Tolerance in the Arsenic Hyperaccumulating Fern Pteris vittata Is Missing in Flowering Plants

Cited by 247 publications

References 76 publications

Stable Transformation of Ferns Using Spores as Targets: Pteris vittata and Ceratopteris thalictroides

Stable Transformation of Ferns Using Spores as Targets: Pteris vittata and Ceratopteris thalictroides

Abscisic acid controlled sex before transpiration in vascular plants

Arsenic Speciation in Phloem and Xylem Exudates of Castor Bean

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