A UPLC-MS/MS-based metabolomics analysis of the pharmacological mechanisms of rabdosia serra against cholestasis

Zhang, Kaihui; Yao, Yufeng; Wang, Meiqi; Liu, Fangle; Wang, Qian; Ma, Huanhuan; Xie, Yuanyuan; Ma, Yunxia; Dai, Pengyu; Chang, Zhu; Chen, Lin

doi:10.1016/j.phymed.2021.153683

Cited by 18 publications

(8 citation statements)

References 38 publications

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“…Extravasation of bile acids and elevation of conjugated bile acids may be maternal adaptive mechanisms during cholestasis to reduce bile acids toxicity to the liver [ 31 ]. Studies have also demonstrated that serum conjugated bile acids significantly increased in various hepatopathies [ 32 – 34 ]. In ICP, especially severe ICP, fetal complications are more closely associated with serum TBA levels.…”

Section: Discussionmentioning

confidence: 99%

Application of metabolomics in intrahepatic cholestasis of pregnancy: a systematic review

et al. 2022

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Background Intrahepatic cholestasis of pregnancy (ICP) is a severe idiopathic disorder of bile metabolism; however, the etiology and pathogenesis of ICP remain unclear. Aims This study comprehensively reviewed metabolomics studies related to ICP, to help in identifying the pathophysiological changes of ICP and evaluating the potential application of metabolomics in its diagnosis. Methods Relevant articles were searched through 2 online databases (PubMed and Web of Science) from January 2000 to March 2022. The metabolites involved were systematically examined and compared. Pathway analysis was conducted through the online software MetaboAnalyst 5.0. Results A total of 14 papers reporting 212 metabolites were included in this study. There were several highly reported metabolites: bile acids, such as glycocholic acid, taurochenodeoxycholic acid, taurocholic acid, tauroursodeoxycholic acid, and glycochenodeoxycholic acid. Dysregulation of metabolic pathways involved bile acid metabolism and lipid metabolism. Metabolites related to lipid metabolism include phosphatidylcholine, phosphorylcholine, phosphatidylserine, sphingomyelin, and ceramide. Conclusions This study provides a systematic review of metabolomics of ICP and deepens our understanding of the etiology of ICP.

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Section: Discussionmentioning

confidence: 99%

Application of metabolomics in intrahepatic cholestasis of pregnancy: a systematic review

et al. 2022

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“…The contents of enmein, epinodosin, and isodocarpin in RSE extract were analyzed by HPLC at the concentrations of 23.95 , 29.94 , and 112.61 mg/g, respectively (shown in Supplementary Figure S1 ). The dose of RSE was determined based on the dosage conversion coefficient of rats according to our previous study ( Zhang et al, 2021 ), and a dose of 300 mg/kg was applied in this study.…”

Section: Methodsmentioning

confidence: 99%

“…Among them, liver protection and anti-inflammatory effects of R. serra are the most prominent (Sun et al, 2020). Our previous study also showed that R. serra extract (RSE) could significantly reduce the levels of AST and ALT and attenuate liver injury induced by concanavalin A (Con A) (He et al, 2016) and alphanaphthylisothiocyanate (ANIT) (Zhang et al, 2021). However, there is a limited understanding of the comparative pharmacokinetic performance of RSE in physiological and pathological states.…”

Section: Introductionmentioning

confidence: 99%

Comparative Pharmacokinetics of Three Bioactive Diterpenoids of Rabdosia serra Extract in Normal and Con A-Induced Liver Injury Rats Using UPLC-MS/MS

et al. 2022

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Rabdosia serra (Maxim.) Hara (R. serra), one of the source plants of “Xihuangcao”, has been widely used as a Chinese folk herb with the concomitant function of both medicine and foodstuff for the prevention and treatment of liver disease. Diterpenoids were considered as the major bioactive components in R. serra, responsible for their effect on hepatoprotection in previous phytochemical and pharmacological studies, while few comparative pharmacokinetic studies have been conducted under the physiological and pathological conditions. To reveal the difference in the pharmacokinetics process of R. serra extract (RSE) in normal and Con A-induced liver injury rats, a rapid ultra-high-pressure liquid chromatography–tandem mass spectrometry method (total running time: 5 min) was established to simultaneously determine three bioactive diterpenoids (enmein, epinodosin, and isodocarpin) in rat plasma. The results showed significant differences in the pharmacokinetic properties of three analytes between the physiological and pathological states. Compared with normal rats, the AUC of the three analytes was remarkably higher in liver injury rats, while the Tmax, T1/2, and MRT were shortened. It indicated that RSE has higher exposure and quicker elimination in liver injury rats than that in normal rats. Our results suggested that the pharmacokinetics of hepatoprotective medications was affected by liver injury, which prospected to provide essential information for guiding the healthcare and clinical application of R. serra in pathological states.

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“…RS treatment modulates the levels of bile acids in the serum and liver and restores the proportion of bile acids in the bile tubes, especially CA and conjugated bile acids such as TCA and GCA. 102 Ligustrum robustum (LR), a flavonoid-rich tea like plant, increases the abundance of the genus Bifidobacterium , which generates BSH, and thus presumably enhances bile acid (BA) deconjugation and increases fecal BA excretion. Meanwhile, LR increases fecal cholesterol excretion, decreases the levels of serum and hepatic cholesterol, but did not affect short-chain fatty acids in feces.…”

Section: Dietary Supplements and Medical Constituents Modulate Bsh-pr...mentioning

confidence: 99%

Targeting gut microbial bile salt hydrolase (BSH) by diet supplements: new insights into dietary modulation of human health

Yang

2022

Food Funct.

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A UPLC-MS/MS-based metabolomics analysis of the pharmacological mechanisms of rabdosia serra against cholestasis

Cited by 18 publications

References 38 publications

Application of metabolomics in intrahepatic cholestasis of pregnancy: a systematic review

Application of metabolomics in intrahepatic cholestasis of pregnancy: a systematic review

Comparative Pharmacokinetics of Three Bioactive Diterpenoids of Rabdosia serra Extract in Normal and Con A-Induced Liver Injury Rats Using UPLC-MS/MS

Targeting gut microbial bile salt hydrolase (BSH) by diet supplements: new insights into dietary modulation of human health

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