A universal polymerase chain reaction for the detection of psittacine beak and feather disease virus

Ypelaar, Ingrid; Bassami, Mohammad Reza; Wilcox, G. E.; Raidal, Shane R.

doi:10.1016/s0378-1135(99)00070-x

Cited by 99 publications

(101 citation statements)

References 14 publications

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“…Blood taken at the nest prior to collection was positive on PCR for BFDV, whereas feathers did not cause hemagglutination (HA) and no response was observed on hemagglutination inhibition (HI) assay of serum. The PBFD diagnostic assays were carried out by the Veterinary Diagnostic Laboratory, Charles Sturt University, following published methods (Ypelaar et al, 1999;Khalesi et al, 2005). These tests have formed the basis for intensive monitoring of wild Orange-bellied Parrots for BFDV since 2000.…”

Section: Methodsmentioning

confidence: 99%

“…Alignment and construction of the full genome sequence was carried out in a pairwise method with overlapping of between 265 and 394 nucleotides between the sequenced segments. Each aligned pair had no ambiguities Ypelaar et al (1999).…”

Section: Methodsmentioning

confidence: 99%

“…The small genome of the virus facilitates whole-genome viral epidemiologic analysis. Like RNA and other ssDNA viruses, BFDV is prone to a high rate of genetic mutation, although the Rep gene is relatively conserved, which conveniently assists with diagnosing infection by PCR detection methods (Ypelaar et al, 1999). Within Psittaciformes, BFDV exhibits quasispecies characteristics with emerging geographic or host specificity demonstrable within various clades (Varsani et al, 2011) and the observed occurrence of closely related clades in highly divergent parrot species is evidence of host switching or host generalism in several BFDV lineages (Varsani et al, 2011;Julian et al, 2012;Kundu et al, 2012;Massaro et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

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Evidence of Psittacine Beak and Feather Disease Virus Spillover Into Wild Critically Endangered Orange-Bellied Parrots (Neophema Chrysogaster)

Peters

Patterson

Baker

et al. 2014

Journal of Wildlife Diseases

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ABSTRACT:We report the recent emergence of a novel beak and feather disease virus (BFDV) genotype in the last remaining wild population of the critically endangered Orange-bellied Parrot (Neophema chrysogaster). This virus poses a significant threat to the recovery of the species and potentially its survival in the wild. We used PCR to detect BFDV in the blood of three psittacine beak and feather disease (PBFD)-affected wild Orange-bellied Parrot fledglings captured as founders for an existing captive breeding recovery program. Complete BFDV genome sequence data from one of these birds demonstrating a 1,993-nucleotide-long read encompass the entire circular genome. Maximum-likelihood (ML) and neighbor-joining (NJ) phylogenetic analysis supported the solitary position of this viral isolate in a genetically isolated branch of BFDV. On Rep gene sequencing, a homologous genotype was present in a second wild orange-bellied parrot and the third bird was infected with a distantly related genotype. These viruses have newly appeared in a population that has been intensively monitored for BFDV for the last 13 yr. The detection of two distinct lineages of BFDV in the remnant wild population of Orange-bellied Parrots, consisting of fewer than 50 birds, suggests a role for other parrot species as a reservoir for infection by spillover into this critically endangered species. The potential for such a scenario to contribute to the extinction of a remnant wild animal population is supported by epidemiologic theory.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Evidence of Psittacine Beak and Feather Disease Virus Spillover Into Wild Critically Endangered Orange-Bellied Parrots (Neophema Chrysogaster)

Peters

Patterson

Baker

et al. 2014

Journal of Wildlife Diseases

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“…The disease is caused by PBFD virus, which belongs to a new virus family named Circoviridae. The virions are small (14 to 17 nm in diameter), non-enveloped and icosahedral, and have circular single-stranded DNA (1.7 to 2.0 kb) [1,11,16]. Biological characters of the virus is similar to those of porcine circovirus (PCV), chicken anemia virus, pigeon circovirus, and some plants viruses [2,8].…”

mentioning

confidence: 99%

“…However, only PCV and PBFD were assigned to genus circovirus [11,12]. Major clinical signs of PBFD were feather dystrophy and loss as well as immunosuppression [14,16]. This report deals with the first onset of PBFD in three captive cockatoos in Thailand and the detection of the viral DNA sequences from the formalin-fixed paraffin-embedded (FFPE) tissues [4,5] by nested polymerase chain reaction (PCR) method.…”

mentioning

confidence: 99%

Psittacine Beak and Feather Disease in Three Captive Sulphur-Crested Cockatoos (<i>Cacatua galerita</i>) in Thailand

Kiatipattanasakul-Banlunara

Tantileartcharoen

Katayama

et al. 2002

The Journal of Veterinary Medical Science

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ABSTRACT. Three sulphur-crested cockatoos (Cacatua galerita) were diagnosed as psittacine beak and feather disease (PBFD). Histopathology of the feather pulp and follicles showed intracytoplasmic botryoid clusters or granular inclusion bodies in epithelial cells and macrophages. Electron microscopy revealed multiple cytoplasmic clusters of electron dense viral particles corresponding to the inclusions. PBFD virus (circovirus) DNA-specific product was detected from formalin-fixed paraffin-embedded feathers by nested polymerase chain reaction (PCR) method. KEY WORDS: circovirus, cockatoo, psittacine beak and feather disease (PBFD).

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Chicken Infectious Anemia Virus and Other Circovirus Infections

Schat

Woods

2013

Diseases of Poultry

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A universal polymerase chain reaction for the detection of psittacine beak and feather disease virus

Cited by 99 publications

References 14 publications

Evidence of Psittacine Beak and Feather Disease Virus Spillover Into Wild Critically Endangered Orange-Bellied Parrots (Neophema Chrysogaster)

Evidence of Psittacine Beak and Feather Disease Virus Spillover Into Wild Critically Endangered Orange-Bellied Parrots (Neophema Chrysogaster)

Psittacine Beak and Feather Disease in Three Captive Sulphur-Crested Cockatoos (<i>Cacatua galerita</i>) in Thailand

Chicken Infectious Anemia Virus and Other Circovirus Infections

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