A uninucleate Rhizoctonia sp. from maize plant with ITS heterogeneity and hypersensitive to abiotic stresses

Zhou, Shijiao; Zhang, Mei; Liu, Ying; Ji, Zhen; Liang, Wenxing; Chen, Xujun; Guo, Zhai; Bao-du, LI

doi:10.1007/s10658-015-0610-2

Cited by 12 publications

(10 citation statements)

References 12 publications

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“…Earlier studies showed that most Rhizoctonia sp. are globally distributed soil-borne fungi which can infect many economically important field crops 22 . Cultivation-based analysis showed that the fungal density was positively correlated with the root gall number and decreased in the HDZ-9-47, biofumigation, and combination treatments relative to the control.…”

Section: Discussionmentioning

confidence: 99%

The effect of combined application of Streptomyces rubrogriseus HDZ-9-47 with soil biofumigation on soil microbial and nematode communities

Jin

Wang

et al. 2019

Sci Rep

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Meloidogyne incognita causes significant damage to many different crops. Previous studies showed that Streptomyces rubrogriseus HDZ-9-47 is a promising biocontrol agent. Combining it with biofumigation improved its efficacy against M. incognita. In the present study, the reason for the improved efficacy of the combination was investigated by analyzing its impact on both the soil microbial and the nematode communities in the field. The results showed that the combined application reduced root galls by 41% and its control efficacy was greater than each treatment alone. Cultivation-based analyses showed that the combination treatment affected the soil microbial community. Actinomycetes and bacterial densities were negatively correlated with the root knot score. In contrast, the fungal densities were positively correlated with the root knot score. Denaturing gradient gel electrophoresis (DGGE) results showed that the combination of S. rubrogriseus HDZ-9-47 and biofumigation enriched beneficial microbes and reduced certain soil-borne fungal phytopathogens, thereby enhancing the efficacies of both S. rubrogriseus HDZ-9-47 and biofumigation against M. incognita. And HDZ-9-47 could colonize in soil. The total abundance of nematode and plant parasites, the ratio of soil fungivore nematode to fungivore plus bacterivore nematode, and the nematode diversity indices all decreased with the combination treatment. Overall, the results of this study demonstrate that combined application of HDZ-9-47 with biofumigation was a useful and effective approach to suppress M. incognita by manipulating soil microbial communities in field.

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Section: Discussionmentioning

confidence: 99%

The effect of combined application of Streptomyces rubrogriseus HDZ-9-47 with soil biofumigation on soil microbial and nematode communities

Jin

Wang

et al. 2019

Sci Rep

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“…Collection of isolates. Rhizoctonia isolates JN, YR, LY, and XN were collected from maize as previously described 21,22,63 , and isolate RW from wheat was kindly provided by Dr. W. Li 64 . Isolate SM was obtained from a blighted maize sheath near Beijing, China.…”

Section: Methodsmentioning

confidence: 99%

“…In previous genome sequencing and assembly efforts using short-reads data 19,20 , the occurrence of multinucleate R. solani of primarily heterokaryotic and diploid features were hindrances to complete genome assembly. Therefore, we sequenced genomes of field isolates of Rhizoctonia of different nuclear types: uninucleate (JN, SM, and YR) 21,22 , binucleate (LY and RW), and multinucleate (XN), among those JN, LY, and XN were assembled to chromosome-scale. Unexpectedly, each uninucleate isolate had a genome assembly that was twice the size of the binucleate isolate, LY.…”

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Evolutionary and genomic comparisons of hybrid uninucleate and nonhybrid Rhizoctonia fungi

et al. 2021

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The basidiomycetous fungal genus, Rhizoctonia, can cause severe damage to many plants and is composed of multinucleate, binucleate, and uninucleate species differing in pathogenicity. Here we generated chromosome-scale genome assemblies of the three nuclear types of Rhizoctonia isolates. The genomic comparisons revealed that the uninucleate JN strain likely arose by somatic hybridization of two binucleate isolates, and maintained a diploid nucleus. Homeolog gene pairs in the JN genome have experienced both decelerated or accelerated evolution. Homeolog expression dominance occurred between JN subgenomes, in which differentially expressed genes show potentially less evolutionary constraint than the genes without. Analysis of mating-type genes suggested that Rhizoctonia maintains the ancestral tetrapolarity of the Basidiomycota. Long terminal repeat-retrotransposons displayed a reciprocal correlation with the chromosomal GC content in the three chromosome-scale genomes. The more aggressive multinucleate XN strain had more genes encoding enzymes for host cell wall decomposition. These findings demonstrate some evolutionary changes of a recently derived hybrid and in multiple nuclear types of Rhizoctonia.

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“…are binucleate or multinucleate. However, few studies have reported UN Rhizoctonia spp., which have been rarely isolated from conifer seedlings, turf‐grasses and maize (Lilja, ; Shi, Shang, & Zhang, ; Zhou et al., ). Each group is composed of different anastomosis groups (AGs).…”

Section: Introductionmentioning

confidence: 99%

Characterization and pathogenicity ofRhizoctoniaspp. isolated from kiwifruit in the Middle and Eastern Black Sea region of Turkey

Türkkan

Erper

Kılıçoğlu

et al. 2018

Journal of Phytopathology

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In this study, 58 Rhizoctonia isolates were obtained from plants exhibiting symptoms of root and stem rot in kiwifruit orchards in six different provinces in the Black Sea region of Turkey. The isolates were analysed for their cultural characteristics, anastomosis groups (AGs) and subgroups according to nucleotide sequence variations in the internal transcribed spacer (ITS) regions including the 5.8S gene of nuclear ribosomal DNA (rDNA) and pathogenicity. Based on ITS1‐5.8S‐ITS2 rDNA sequencing, 52 binucleate (BN) Rhizoctonia isolates were identified as belonging to AG‐O (17), AG‐L (1), AG‐G (7), AG‐I (1), AG‐A (9), AG‐Fa (9), AG‐Fb (1), AG‐R (3) and AG‐P (4). Of the remaining 6 isolates, three belonged to multinucleate (MN) R. solani AG 4 HG‐I, one belonged to AG 4 HG‐II, one belonged to AG 5 and one belonged to AG 1‐IB. The sequences of these isolates were aligned and compared with other Rhizoctonia sequences retrieved from GenBank (NCBI), and phylogenetic analysis was used to determine evolutionary relationships among them. All isolates used in the temperature experiments grew between 15–35°C, and their growth was completely inhibited at 5 and 40°C, with a few exceptions. In the pathogenicity tests carried out using 58.6% of the isolates on kiwifruit seedlings, it was determined that there were statistically significant virulence differences among BN and MN Rhizoctonia isolates (p < 0.05). The virulence of BN Rhizoctonia ranged from 0.2 to 4.0, whereas that of MN varied from 2.4 to 4.0. Unlike some isolates of AG‐R, AG 4 HG‐II and AG 1‐IB, which had no significant difference between them in virulence, AG‐P and AG 4 HG‐I isolates significantly reduced plant height, shoot and root dry weights and root length when compared to control plants. To our knowledge, it is the first detailed report of AGs of BN and MN Rhizoctonia, the causal agent of root and stem rot on kiwifruit in Turkey and in the world.

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A uninucleate Rhizoctonia sp. from maize plant with ITS heterogeneity and hypersensitive to abiotic stresses

Cited by 12 publications

References 12 publications

The effect of combined application of Streptomyces rubrogriseus HDZ-9-47 with soil biofumigation on soil microbial and nematode communities

The effect of combined application of Streptomyces rubrogriseus HDZ-9-47 with soil biofumigation on soil microbial and nematode communities

Evolutionary and genomic comparisons of hybrid uninucleate and nonhybrid Rhizoctonia fungi

Characterization and pathogenicity ofRhizoctoniaspp. isolated from kiwifruit in the Middle and Eastern Black Sea region of Turkey

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