A Two-Photon Fluorescent Probe for Thiols in Live Cells and Tissues

Abstract

We report a two-photon fluorescent probe (ASS) that can be excited by 780 nm femtosecond pulses and detect thiols in live cells and living tissues at a 90-180 microm depth without interference from other biologically relevant species by two-photon microscopy.

Photocontrollable fluorogenic probes for visualising near-membrane copper(ii) in live cells

“…6d). 34 Fluorescence intensities also exhibited a linear relationship with the copper(II) concentration, as shown in Fig. S7 † (R 2 ¼ 0.97).…”

Sensing for intracellular thiols by water-insoluble two-photon fluorescent probe incorporating nanogel

“…Near-infrared (NIR) excitation within the range of the optical window in tissues (approximately 700-1000 nm), in which the absorption and scattering by tissues are low, enables deeper tissue penetration and reduces photoxicity. Therefore, the probe with two-photon fluorescence is considered to be an attractive solution to surmount the drawbacks of single-photon excitation [23].…”

Chromogenic and fluorogenic chemosensors and reagents for anions. A comprehensive review of the years 2010–2011

“…266) for the fluorogenic recognition of thiols. 325 Aqueous solutions of 506 (MOPS buffer, pH 7.2) showed a weak emission at 450…”