A transcription aptasensor: amplified, label-free and culture-independent detection of foodborne pathogens <i>via</i> light-up RNA aptamers

Sheng, Lele; Lu, Yunhao; Deng, Sha; Liao, Xinyu; Zhang, Kaixiang; Ding, Tian; Gao, Hong; Liu, Donghong; Deng, Ruijie; Li, Jinghong

doi:10.1039/c9cc05036a

Cited by 46 publications

(34 citation statements)

References 37 publications

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“…These receptors at normal surface concentrations (1-5 mg/mm 2 ) are immobilized on a solid sensor substrate (matrix) by covalent binding or biotin-avidin interaction. They serve for selective binding and identification of target analytes (ligands) in biological fluids (whole blood, serum, plasma, urine, saliva, liquor, tissue extracts, and cell cultures) [3,5,[52][53][54].…”

Section: Main Types Of Biosensors and Their Functionsmentioning

confidence: 99%

“…The widespread use of label-free biosensor was facilitated to a large extent by the fact that they use biological or chemical receptors for direct detection of the analyzed molecules in the sample, without the use of special enzyme and radioactive or fluorescent labels. They allow screening of intermolecular interactions and cellular reactions, providing detailed information about the selectivity of effect of bacterial exotoxins and the specificity of antimicrobial agents, the antigen-antibody interaction, as well as the kinetics of inflammatory process, immunological, and serological reactions [3,40,75,76].…”

Section: Label-free Biosensorsmentioning

confidence: 99%

“…Optical, electrochemical, electric (piezoelectric), microwave, or (micro) mechanical transducers are promising strategies for recognizing ligand-receptor interaction signals in label-free biosensors used to indicate biological agents or biomonitoring the environment [32,40,80,84]. Among the most powerful detection and analysis tools, widely used in biomedical research and in practical medicine, are biosensors with optical transducers, considered the basic tool for signal perception [3,7,75,89,90].…”

Section: Label-free Biosensorsmentioning

confidence: 99%

“…Monitoring and early identification of markers is essential for the diagnosis of infectious diseases. Therefore, the study of molecular markers of biological agents, as well as the search and development of highly effective and sensitive methods for indicating pathogenic microorganisms, have always been the focus of attention of researchers [1][2][3].…”

Section: Introductionmentioning

confidence: 99%

“…Many years of world experience in using modern biosensors for specific indication of pathological biological agents have shown that these devices have a high potential to become fast and reliable in operation, highly specific and sensitive tools for timely diagnosis [3,5,6,8]. They fully comply with the modern «point-of-care testing» concept and can be successfully applied in addressing biosafety issues and emergency indication in emergency situations of natural or man-made origin to protect the population or the environment [8,9].…”

Section: Introductionmentioning

confidence: 99%

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Label-Free Biosensors for Laboratory-Based Diagnostics of Infections: Current Achievements and New Trends

et al. 2020

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Infections pose a serious global public health problem and are a major cause of premature mortality worldwide. One of the most challenging objectives faced by modern medicine is timely and accurate laboratory-based diagnostics of infectious diseases. Being a key factor of timely initiation and success of treatment, it may potentially provide reduction in incidence of a disease, as well as prevent outbreak and spread of dangerous epidemics. The traditional methods of laboratory-based diagnostics of infectious diseases are quite time- and labor-consuming, require expensive equipment and qualified personnel, which restricts their use in case of limited resources. Over the past six decades, diagnostic technologies based on lateral flow immunoassay (LFIA) have been and remain true alternatives to modern laboratory analyzers and have been successfully used to quickly detect molecular ligands in biosubstrates to diagnose many infectious diseases and septic conditions. These devices are considered as simplified formats of modern biosensors. Recent advances in the development of label-free biosensor technologies have made them promising diagnostic tools that combine rapid pathogen indication, simplicity, user-friendliness, operational efficiency, accuracy, and cost effectiveness, with a trend towards creation of portable platforms. These qualities exceed the generally accepted standards of microbiological and immunological diagnostics and open up a broad range of applications of these analytical systems in clinical practice immediately at the site of medical care (point-of-care concept, POC). A great variety of modern nanoarchitectonics of biosensors are based on the use of a broad range of analytical and constructive strategies and identification of various regulatory and functional molecular markers associated with infectious bacterial pathogens. Resolution of the existing biosensing issues will provide rapid development of diagnostic biotechnologies.

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Section: Main Types Of Biosensors and Their Functionsmentioning

confidence: 99%

Section: Label-free Biosensorsmentioning

confidence: 99%

Section: Label-free Biosensorsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Label-Free Biosensors for Laboratory-Based Diagnostics of Infections: Current Achievements and New Trends

et al. 2020

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Quantum Dots for Pathogenic Bacterial Monitoring and Combating

Liu

Zhang

2022

Advanced Optical Materials

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including neonatal meningitis, [6] diarrhoea, [7] infected cutaneous wound [8] or bloodstream diseases, [9][10][11] etc. Antibiotic treatment strategies against these bacteria have been widely applied while antimicrobial resistance has been arousing gradually. [12][13][14][15][16][17] What is worse, the biofilmrelated infection has been a tough issue for bacterial infectious treatment since it is usually chronic and much harder to eradicate. [18][19][20] Therefore, effective antibacterial treatments for drug-resistant pathogenic bacteria are vital problems for clinical therapy. [21] Some anti-microbial trials were carried out like phages, bacteriocins, and antimicrobial peptides [22] along with various therapeutic methods. [23][24][25] Among them, nanomaterials are considered as compatible solutions [23,26] due to their facile synthesis methods and rich-sourced precursors, broad-spectrum sterilization, and low drug resistance. [27][28][29][30][31] Nanomaterials are generally easy to be modified or doped to enhance the affinity toward bacteria for the sake of combating or drug delivery. [32][33][34][35] The antibacterial mechanism [36,37] could be result from the sharp edges-based physical cutting, [38,39] photothermal effects, [40][41][42] and photo dynamic or chemical dynamic properties, that could produce reactive oxygen species (ROS).Pathogenic bacteria monitoring mainly conducted by culturing them in agar-based media, and subsequently testing them with biochemical probes. [43][44][45][46] Although this conventional method enables clinical diagnoses with relatively reliable outcomes, the long culturing time renders the "golden therapeutical window" for patients. So more rapid and accurate detecting technologies have been invented. One of the most evolutionary methods is the polymerase chain reaction, [47][48][49] which can identify the bacterial classification and provide gene expression level in a few hours with better accuracy. [47,50] Besides, the coupling of chromatography and mass spectra like matrix-assisted laser desorption ionization time-of-flight mass spectrometry [51][52][53][54][55] can play a more exact role in diagnosis. [56] Though these advanced instrumental-relied methods can lead to fast diagnostic, the expensive apparatus, environmentally unfriendly reagents, [57] professional sites and technical personnel requirements limited their utilization in real-time monitoring and point-of-care sensing. By contrast, easy and fast monitoring methods for the pathogen, such as electrochemical and fluorescence sensors, were brought out as powerful supplements. [58][59][60][61][62] Therein, Antibiotic resistance and pathogenic bacterial monitoring are two main challenges in clinical diagnostics and treatment of bacteria. To conquer those issues, many new materials and novel technologies are explored. Among them, quantum dots (QDs) are regarded as not only powerful bacteriostatic agents, but also bacterial monitoring probes due to their excellent photoluminescence properties, tunable multiwavelength lumine...

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Signal‐off Photoelectrochemical Aptasensor for S. aureus Detection Based on Graphite‐like Carbon Nitride Decorated with Nickel Oxide

Chen

Yang

et al. 2021

Electroanalysis

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A signal‐off photoelectrochemical (PEC) aptasensor was proposed for pathogenic bacteria detection based on graphite‐like carbon nitride decorated with NiO. The S. aureus be captured by the specific recognition of DNA aptamer, which was immobilized on the PEC electrode surface according to the specific effect of carboxylic group and NiO. The PEC signal would be obviously suppressed due to the transfer blocking of electron donor to PEC active material surface by steric hindrance and electrostatic repulsion. The PEC aptasensor showed excellent specificity and high sensitivity for S. aureus, and this provides simple strategy to construct point‐of‐care testing aptasensor for bacteria analysis.

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A transcription aptasensor: amplified, label-free and culture-independent detection of foodborne pathogens via light-up RNA aptamers

Abstract: We report a light-up RNA aptamer-based transcription aptasensor, enabling sensitive, label-free and culture-free detection of intact foodborne pathogens.

Cited by 46 publications

References 37 publications

Label-Free Biosensors for Laboratory-Based Diagnostics of Infections: Current Achievements and New Trends

Label-Free Biosensors for Laboratory-Based Diagnostics of Infections: Current Achievements and New Trends

Quantum Dots for Pathogenic Bacterial Monitoring and Combating

Signal‐off Photoelectrochemical Aptasensor for S. aureus Detection Based on Graphite‐like Carbon Nitride Decorated with Nickel Oxide

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