A tradeoff between tolerance and resistance to a major fungal pathogen in elite wheat cultivars

Mikaberidze, Alexey; McDonald, Bruce A.

doi:10.1101/354357

Cited by 4 publications

(9 citation statements)

References 74 publications

(14 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…ρ leaf represents the overall reproductive success per area while ρ lesion focuses on the reproductive success within the lesion area. Tolerance indicates the ability of the host to tolerate pathogen reproduction while limiting damage by lesions (Mikaberidze & McDonald, 2020). We found that the mean pycnidia count ranged from 0-20 (mean 7, median 6.3) among isolates and PLACP ranged from 2-97 % (mean 56%, median 57.7%) (Fig.…”

Section: Heritability and Correlations Among Pathogenicity Traitsmentioning

confidence: 83%

“…Furthermore, variation in the pathogen's ability to spread across tissue and manipulate host immune responses could also lead to variation in overall lesion development. Interestingly, extensive lesion development is not necessarily related to pycnidia production by the pathogen across cultivars (Karisto et al, 2018;Mikaberidze & McDonald, 2020). This suggests that despite damage to the leaf, the host immune system can e ciently repress the pathogen from acquiring nutrients to reproduce.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Rapid sequence evolution driven by transposable elements at a virulence locus in a fungal wheat pathogen

Singh

Badet

Croll

2021

Preprint

View full text Add to dashboard Cite

Background: Plant pathogens cause substantial crop losses in agriculture production and threaten food security. Plants evolved the ability to recognize virulence factors and pathogens have repeatedly escaped recognition due rapid evolutionary change at pathogen virulence loci (i.e. effector genes). The presence of transposable elements (TEs) in close physical proximity of effector genes can have important consequences for gene regulation and sequence evolution. Species-wide investigations of effector gene loci remain rare hindering our ability to predict pathogen evolvability.Results: Here, we performed genome-wide association studies (GWAS) on a highly polymorphic mapping population of 120 isolates of Zymoseptoria tritici, the most damaging pathogen of wheat in Europe. We identified a major locus underlying significant variation in reproductive success of the pathogen and damage caused on the wheat cultivar Claro. The most strongly associated locus is intergenic and flanked by genes encoding a predicted effector and a serine type protease, respectively. The center of the locus contained a highly dynamic region consisting of multiple families of TEs. Based on a large global collection of assembled genomes, we show that the virulence locus has undergone substantial recent sequence evolution. Large insertion and deletion events generated length variation between the flanking genes by a factor of seven (5–35 kb). The locus showed also strong signatures of genomic defenses against TEs (i.e. RIP) contributing to the rapid diversification of the locus.Conclusions: In conjunction, our work highlights the power of combining GWAS and population-scale genome analyses to investigate major effect loci in pathogens.

show abstract

Section: Heritability and Correlations Among Pathogenicity Traitsmentioning

confidence: 83%

Section: Discussionmentioning

confidence: 99%

Rapid sequence evolution driven by transposable elements at a virulence locus in a fungal wheat pathogen

Singh

Badet

Croll

2021

Preprint

View full text Add to dashboard Cite

show abstract

“…Furthermore, variation in the pathogen's ability to spread across tissue and manipulate host immune responses could also lead to variation in overall lesion development. Interestingly, extensive lesion development is not necessarily related to pycnidia production by the pathogen across cultivars (Karisto et al, 2018;Mikaberidze & McDonald, 2020). This suggests that despite damage to the leaf, the host immune system can efficiently repress the pathogen from acquiring nutrients to reproduce.…”

Section: Discussionmentioning

confidence: 98%

“…rleaf represents the overall reproductive success per area while rlesion focuses on the reproductive success within the lesion area. Tolerance indicates the ability of the host to tolerate pathogen reproduction while limiting damage by lesions (Mikaberidze & McDonald, 2020). We found that the mean pycnidia count ranged from 0-20 (mean 7, median 6.3) among isolates and PLACP ranged from 2-97 % (mean 56%, median 57.7%) (Fig 1C, We estimated SNP-based heritability (h 2 snp) for each trait using a genomic-relatedness-based restricted maximum-likelihood approach to partition the observed phenotypic variation (Fig 1D).…”

Section: Heritability and Correlations Among Pathogenicity Traitsmentioning

confidence: 99%

Rapid sequence evolution driven by transposable elements at a virulence locus in a fungal wheat pathogen

Singh

Badet

Croll

2021

Preprint

View full text Add to dashboard Cite

Plant pathogens cause substantial crop losses in agriculture production and threaten food security. Plants evolved the ability to recognize virulence factors and pathogens have repeatedly escaped recognition due rapid evolutionary change at pathogen virulence loci (i.e. effector genes). The presence of transposable elements (TEs) in close physical proximity of effector genes can have important consequences for gene regulation and sequence evolution. Species-wide investigations of effector gene loci remain rare hindering our ability to predict pathogen evolvability. Here, we performed genome-wide association studies (GWAS) on a highly polymorphic mapping population of 120 isolates of Zymoseptoria tritici, the most damaging pathogen of wheat in Europe. We identified a major locus underlying significant variation in reproductive success of the pathogen and damage caused on the wheat cultivar Claro. The most strongly associated locus is intergenic and flanked by genes encoding a predicted effector and a serine type protease, respectively. The center of the locus contained a highly dynamic region consisting of multiple families of TEs. Based on a large global collection of assembled genomes, we show that the virulence locus has undergone substantial recent sequence evolution. Large insertion and deletion events generated length variation between the flanking genes by a factor of seven (5-35 kb). The locus showed also strong signatures of genomic defenses against TEs (i.e. RIP) contributing to the rapid diversification of the locus. In conjunction, our work highlights the power of combining GWAS and population-scale genome analyses to investigate major effect loci in pathogens.

show abstract

“…(Kefauver et al, 2015). As aplicações de imagens de órgãos de plantas são comuns na literatura e inclusive utilizadas para mensurar a tolerância de plantas a doenças (Mikaberidze e McDonald, 2018).…”

Section: Fenotipagem De Alto Rendimento No Melhoramento De Plantasunclassified

Análises genéticas revelam oportunidades e obstáculos da tolerância ao fungo da ferrugem asiática da soja

Bornhofen¹

View full text Add to dashboard Cite

Análises genéticas revelam oportunidades e obstáculos da tolerância ao fungo da ferrugem asiática da soja / Elesandro Bornhofen-Piracicaba, 2019. 126 p. Tese (Doutorado)-USP / Escola Superior de Agricultura "Luiz de Queiroz". 1. Análise espacial 2. Estresse biótico 3. Interação genótipos por ambientes 4. Glycine max 5. Phakopsora pachyrhizi. I. Título DEDICATÓRIA Essa tese é especialmente dedicada aos meus pais, Ari e Ivanir, por todo o suporte e amor ao longo da vida, e a minha avó paterna, Angelina, pelo imenso carinho. Eu também dedico essa tese aos muitos amigos que contribuíram para tornar essa esplêndida jornada mais divertida. Por fim, porém não menos importante, dedico esses anos de trabalho, aqui sumarizados em alguns poucos milhares de palavras, aos agricultores brasileiros, empresários resilientes e comprometidos em continuamente evoluir a agricultura nacional. AGRADECIMENTOS Inicialmente, gostaria de ressaltar que a existência desta tese é fruto da valiosa contribuição de tantos, dos quais sou muito grato. Agradeço a toda a minha família pelo apoio constante desde o início dessa jornada, especialmente aos meus pais Ari e Ivanir Bornhofen. A minha linda noiva, Dra. Tábata Bergonci, pelo companherismo, inteligência, amor e dedicação, não medindo esforços para me auxiliar em direção ao sucesso. Meu muito obrigado também destina-se a todos os amigos próximos que, quer seja pelos momentos alegres ou difíceis, o certo é que crescemos juntos. Sou imensamente grato ao Professor Dr. Natal A. Vello pelo espaço disponibilizado em seu grupo de pesquisa, o que me proporcionou a oportunidade da conviver com cientistas notáveis, além de suas conversas e valorosas orientações. Estendo também meus agradecimentos ao Professor da Universidade de Minnesota, Ph.D. Aaron J. Lorenz, por ter me recebido como pesquisador visitante em seu grupo e por ter dedicado muito do seu tempo em tornar minha experiência agradável e rica em conhecimentos. Aos professores do Departamento de Genética e dos demais departamentos pelos ensinamentos por meio de aulas agradáveis e conversas informais. Também, agradeço aos colegas da pós-graduação dos vários departamentos da instituição pelos momentos de estudo e pelas amizades conquistadas. Não posso deixar de mencionar as grandes amizades conquistadas durante o estágio no exterior, especialmente dos colegas de moradia (Minnesota Graduate Club), de laboratório e do futebol; sei que estão espalhados pelos quatro cantos do mundo mas espero um dia revê-los. O presente trabalho foi realizado com apoio da Coordenação de Aperfeiçoamento de Pessoal de Nível Superior-Brasil (CAPES)-Código de Financiamento 001, por meio de bolsas no período de 01 de

show abstract

A tradeoff between tolerance and resistance to a major fungal pathogen in elite wheat cultivars

Cited by 4 publications

References 74 publications

Rapid sequence evolution driven by transposable elements at a virulence locus in a fungal wheat pathogen

Rapid sequence evolution driven by transposable elements at a virulence locus in a fungal wheat pathogen

Rapid sequence evolution driven by transposable elements at a virulence locus in a fungal wheat pathogen

Análises genéticas revelam oportunidades e obstáculos da tolerância ao fungo da ferrugem asiática da soja

Contact Info

Product

Resources

About