A time course transcriptome analysis of brains from sex-undifferentiated Nile tilapia discloses genes associated with high-temperature-induced masculinization

Yao, Zhi Lei; Chen, Hong Ju; Zhao, Yan; Cao, Zhen; Wang, Hui; Ji, Xiang

doi:10.1016/j.aquaculture.2020.735762

Cited by 9 publications

(10 citation statements)

References 63 publications

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“…The previously observed upregulation POMC (a pre‐protein which signals for stress hormone production) in sex‐reversed females could have been biased by random acute upregulation of the stress response during manual handling and/or low sample sizes (Deveson et al, 2017). However, similar POMC upregulation occurs in temperature treated fish with a sex reversal system (Yao et al, 2021), corroborating the Deveson et al (2017) result. We assumed that the observed POMC upregulation in sex‐reversed females (Deveson et al, 2017) is indicative of a stress response because one of its derived proteins (ACTH) is released into the circulatory system and signals glucocorticoid secretion from the adrenals.…”

Section: Discussionsupporting

confidence: 70%

Corticosterone does not have a role in temperature sex reversal in the central bearded dragon (Pogona vitticeps)

2021

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Environmental sex determination (ESD) is common among ectothermic vertebrates. The stress axis and production of stress hormones (corticosteroids) regulates ESD in fish, but evidence of a similar influence in reptiles is sparse and conflicting. The central bearded dragon (Pogona vitticeps) has a system of sex determination involving the interplay between sex chromosomes (ZZ/ZW female heterogamety) and the thermal environment. High egg incubation temperatures induce sex reversal of the ZZ genotype, feminizing chromosomally male individuals. Here we show that corticosterone elevation is not associated with sex reversal in the central bearded dragon, either during embryonic development or adulthood. We also demonstrate experimentally that sex determination is not affected by corticosterone injection into the yolk. This strongly suggests that stress axis upregulation by high temperature during incubation does not cause sex reversal in P. vitticeps. Our work is in general agreement with other research in reptiles, which suggests that the stress axis does not mediate sex in reptiles with ESD. Alternative biological systems may be responsible for capturing environmental conditions during reptile development, such as cellular calcium and redox regulation or the action of temperature‐sensitive splicing factors.

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Section: Discussionsupporting

confidence: 70%

Corticosterone does not have a role in temperature sex reversal in the central bearded dragon (Pogona vitticeps)

2021

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“…At high temperatures; however, hyper‐phosphorylated STAT3 bound to the kdm6b promoter, reducing kdm6b expression and blocking dmrt1 expression and male development; thus, leading to female gonad development (Weber et al, 2020). It was also discovered that high‐temperature treatment significantly upregulated the expression of kdm6bb at 5 dah + 24 h and 5 dah + 12 d in tilapia brains (Yao et al, 2021). In the current study, high temperatures upregulated the expression of kdm6bb at 12–25 dpf.…”

Section: Discussionmentioning

confidence: 99%

“…It was discovered that histone demethylation plays a determining role in the TSD of slider turtles (Trachemys scripta) through the function of lysine demethylase 6B (KDM6B) (Ge et al, 2018). The temperature response of kdm6b has also been reported in tilapia (Yao et al, 2021), American alligator (Alligator mississippiensis) (Yatsu et al, 2016) and dragon (Pogona vitticeps) (Deveson et al, 2017). There are other critical players in sex differentiation, for example, the cytochrome P450 aromatase, CYP19A.…”

Section: Members Of the Tgfβ Superfamily Such As Anti-müllerian Hormonementioning

confidence: 99%

Molecular and morphological changes in Nile tilapia ( Oreochromis niloticus ) gonads during high‐temperature‐induced masculinization

et al. 2021

Aquaculture Research

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Genetic sex of Nile tilapia (Oreochromis niloticus) is determined by genotype (genetic sex determination). However, these fish are temperature-sensitive, and hightemperature-induced sex reversal is a common practice in research and tilapia farming.In this study, we characterized the molecular and morphological sex differentiation of Nile tilapia during the process of sex reversal induced by high temperatures. The offspring of parental pairs of XX and XY Nile tilapia underwent high-temperature treatment for 20 d beginning at 5 dpf (days post fertilization). Gonads were isolated from Nile tilapia at seven time points between 10-180 dpf. The histology results showed that gonads of high-temperature-induced XX pseudomales exhibited morphological differences from XX females during 25-35 dpf. qRT-PCR analysis revealed that the expression levels of cyp19a1a and foxl2 were temperature-sensitive, and were lowly expressed in the gonads of pseudomales from 35 dpf onward. Such findings were also consistent with the histological observations of gonadal morphological differentiation. The expression of the TGFβ superfamily member, gsdf, was increased in the gonads of pseudomales from 12 dpf onward. The transcription of kdm6bb was elevated by high-temperature treatment at 10-25 dpf and displayed sexual dimorphism from 35 dpf onward. Dmrt1 was specifically expressed in phenotypic males from 12 dpf onward. Collectively, this study identified the roles of specific genes involved in the process of high-temperature-induced masculinization in Nile tilapia and established robust molecular markers of phenotypic sex in Nile tilapia that will be useful for ongoing research related to sex control.

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“…For female-specific gonad differentiation genes, two DMSs were observed in asns, dapk1 and rpgrip1 and one DMS was observed in ctnnb1, actr6 and smpdl3b (Table 2). In addition, several DMSs were identified in genes related to high-temperature-induced masculinization in Nile tilapia (Oreochromis niloticus) [31]. For example, two DMSs were discovered in Kdm6bb and six DMSs in Jarid2, both of which were up-regulated in male brains when compared with female brains.…”

Section: Dmss Within Sex-related Genes In the Two Tissuesmentioning

confidence: 99%

“…For example, two DMSs were discovered in Kdm6bb and six DMSs in Jarid2, both of which were up-regulated in male brains when compared with female brains. Besides, 18 DMSs were presented in heat shock proteins (HSPs) families, such as hspa2, hspa5 and hspa9, which has been reported to act as a steroid receptor and plays an important role in sex differentiation [31]. In gonad tissues, only one DMS was found in rspol and one DMS in dapk, both of which were female-specific gonad differentiation genes [32].…”

Section: Dmss Within Sex-related Genes In the Two Tissuesmentioning

confidence: 99%

DNA Methylation Difference between Female and Male Ussuri Catfish (Pseudobagrus ussuriensis) in Brain and Gonad Tissues

Chen

Zhu

et al. 2022

Life

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DNA methylation has been found to be involved in sex determination and differentiation in many aquaculture species. The Ussuri catfish (Pseudobagrus ussuriensis) is a popular aquaculture fish in China with high economic value in which male-biased sex dimorphism was observed in terms of body size and body weight. In this study, DNA methylation-sensitive RAD sequencing (Methyl-RAD) was used to explore the epigenetic difference between adult male and female samples in brain and gonad tissues. In brain tissues, 5,442,496 methylated cytosine sites were found and 9.94% of these sites were from symmetric CCGG or CCWGG sites. Among these sites, 321 differential DNA methylation sites (DMSs) in 171 genes were identified, while in gonad tissues, 4,043,053 methylated cytosines sites were found in total and 11.70% of them were from CCGG or CCWGG. Among these sites, 78 differential DNA methylation sites were found which were located in 64 genes. We also found several sex-determination genes among these differential methylated genes, such as amh, gsdf and hsd11b2 in brain tissues and slco3a1, socs2 and trim47 in gonad tissues. These results provided evidence for understanding the function of DNA methylation in the sex differentiation in Pseudobagrus ussuriensis, which further deepens the relationship between gene regulation and epigenetics.

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A time course transcriptome analysis of brains from sex-undifferentiated Nile tilapia discloses genes associated with high-temperature-induced masculinization

Cited by 9 publications

References 63 publications

Corticosterone does not have a role in temperature sex reversal in the central bearded dragon (Pogona vitticeps)

Corticosterone does not have a role in temperature sex reversal in the central bearded dragon (Pogona vitticeps)

Molecular and morphological changes in Nile tilapia ( Oreochromis niloticus ) gonads during high‐temperature‐induced masculinization

DNA Methylation Difference between Female and Male Ussuri Catfish (Pseudobagrus ussuriensis) in Brain and Gonad Tissues

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