A Thin‐Layer Liquid Culture Technique for the Growth of <i>Helicobacter pylori</i>

Joo, Jung-Soo; Park, Kyung-Chul; Song, Jae‐Young; Kim, Dong-Hyun; Lee, Kyung-Ja; Kwon, Young-Cheol; Kim, Jungmin; Kim, Kyungmi; Youn, Hee‐Shang; Kang, Hyung-Lyun; Baik, Seung‐Chul; Cho, Myung-Je; Rhee, Kwang‐Ho

doi:10.1111/j.1523-5378.2010.00767.x

Cited by 16 publications

(9 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The gerbils were euthanized at 3, 6, 12, and 18 months by cervical dislocation under anesthesia to harvest the stomach. Assuming that the H. pylori generation time is at least 3 h (Jiang and Doyle, 2000; Joo et al, 2010), the generation numbers (G) achieved at these months were 654 G, 1309 G, 2617 G, and 3926 G, respectively. The stomach was dissected along the greater curvature and washed with phosphate-buffered saline (PBS pH 7.4, 0.01 M).…”

Section: Methodsmentioning

confidence: 99%

Diversification of the vacAs1m1 and vacAs2m2 Strains of Helicobacter pylori in Meriones unguiculatus

et al. 2016

View full text Add to dashboard Cite

The bacterium Helicobacter pylori exhibits great genetic diversity, and the pathogenic roles of its virulence factors have been widely studied. However, the evolutionary dynamics of H. pylori strains during stomach colonization are not well-characterized. Here, we analyzed the microevolutionary dynamics of the toxigenic strain vacAs1m1, the non-toxigenic strain vacAs2m2, and a combination of both strains in an animal model over time. Meriones unguiculatus were inoculated with the following bacteria: group 1-toxigenic strain vacAs1m1/cagA+/cagE+/babA2+; ST181, group 2-non-toxigenic strain vacAs2m2/cagA+/cagE+/babA2+; ST2901, and group 3-both strains. The gerbils were euthanized at different time points (3, 6, 12, and 18 months). In group 1, genetic alterations were observed at 6 and 12 months. With the combination of both strains, group 3 also exhibited genetic alterations at 3 and 18 months; moreover, a chimera, vacA m1-m2, was detected. Additionally, four new sequence types (STs) were reported in the PubMLST database for H. pylori. Synonymous and non-synonymous mutations were analyzed and associated with alterations in amino acids. Microevolutionary analysis of the STs (PHYLOViZ) identified in each group revealed many mutational changes in the toxigenic (vacAs1m1) and non-toxigenic (vacAs2m2) strains. Phylogenetic assessments (eBURST) did not reveal clonal complexes. Our findings indicate that the toxigenic strain, vacAs1m1, and a combination of toxigenic and non-toxigenic strains acquired genetic material by recombination. The allelic combination, vacAs2m1, displayed the best adaptation in the animal model over time, and a chimera, m1-m2, was also identified, which confirmed previous reports.

show abstract

Section: Methodsmentioning

confidence: 99%

Diversification of the vacAs1m1 and vacAs2m2 Strains of Helicobacter pylori in Meriones unguiculatus

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Beyond traditional culture based research, recent investigations on gastric microbiota employ immunologic, molecular and genetic tools. The presence of Helicobacter heilmannii -associated gastritis was reported in Korea and Japan and the possible association to MALT lymphoma was claimed also in animal model 7. However, we have no evidence of any microbe other than H. pylori in human.…”

mentioning

confidence: 49%

Helicobacter pyloriEradication Therapy, the Reasonable First Line Therapy for Gastric Mucosa-Associated Lymphoid Tissue Lymphoma Irrespective of Infection Status and Disease Stages

Cheung

2016

Gut and Liver

View full text Add to dashboard Cite

“…В кислой среде желудка H. pylori активно синтезирует уреазу, формамидазу, аргиназу, расщепляющие субстраты с образованием аммиака, нейтрализующим соляную кислоту вокруг бактерий [1]. Уреаза H. pylori является несекретируемым цитоплазматическим Ni 2+ -содержащим белком, регулирующим внутреннюю концентрацию протонов Н + посредством повышения рН в периплазме [1,7]. H. pylori способны при аутолизе части бактерий адсорбировать уреазу на поверхности выживших, создавая внеклеточную фракцию фермента [8].…”

Section: физиология Helicobacter Pyloriunclassified

Significance of pathogenicity factors in initiation of immune response in Helicobacter pylori infection

Матвеева

Kapkaeva

Капкаева³

et al. 2020

Vestn. Ross. univ. družby nar., Ser. Med.

View full text Add to dashboard Cite

Helicobacter pylori is a unique microorganism capable of long-term colonization of the gastric mucosa, induction of the inflammatory process, antigenic mimicry and immune evasia. Flagella proteins, adhesins, invasive and aggressive enzymes, cytotoxin-associated protein, vacuolating cytotoxin can have a damaging effect on stomach epithelial cells. Recognition of molecular patterns of Helicobacter pylori by stomach cell receptors initiates activation of adapter proteins, protein kinases and transcription factors, leading to the production of proinflammatory cytokines, infiltration by neutrophilic granulocytes, absorption and killing of microorganisms by phagocytes with presentation of antigens to lymphocytes, while the activity and completeness of phagocytosis remain at a low level. Activation of CD8+-, CD16+- lymphocytes is accompanied by cytotoxic effect on both Helicobacter pylori and epithelial cells of the gastric mucosa. Weak immunogenicity of Helicobacter pylori antigens limits the production of anti-Helicobacter antibodies. Thus, activation of immune factors, in most cases, does not lead to complete elimination of the pathogen, but can aggravate the pathomorphological changes of the gastric epithelium.

show abstract

A Thin‐Layer Liquid Culture Technique for the Growth of Helicobacter pylori

Abstract: Thin-layer liquid culture technique is feasible, and can serve as a versatile liquid culture technique for investigating bacterial properties of H. pylori.

Cited by 16 publications

References 33 publications

Diversification of the vacAs1m1 and vacAs2m2 Strains of Helicobacter pylori in Meriones unguiculatus

Diversification of the vacAs1m1 and vacAs2m2 Strains of Helicobacter pylori in Meriones unguiculatus

Helicobacter pyloriEradication Therapy, the Reasonable First Line Therapy for Gastric Mucosa-Associated Lymphoid Tissue Lymphoma Irrespective of Infection Status and Disease Stages

Significance of pathogenicity factors in initiation of immune response in Helicobacter pylori infection

Contact Info

Product

Resources

About