A technique for the determination of &lt;Superscript&gt;90&lt;/Superscript&gt;Sr, plutonium and americium isotopes in environmental samples

Berlizov, A. N.; Sajeniouk, A. D.; Tryshyn, V. V.

doi:10.1007/s10967-005-0054-9

Cited by 16 publications

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“…The measurements of 90 Sr radioactivity in harvested seeds were carried out using the gravimetric method for β-radiometry and a low-background UMF-1500 M instrument, as previously described. 13 Measurements of γ radioactivity using a semiconductivity coaxial detector of superpure Germanium, with an energetically resolved 1332.5 keV peak of 60 Co, were performed, as we recently described. 3 The transfer coefficient (TC) was calculated by dividing plant radioactivity by soil radioactivity (kBq•kg −1 ).…”

Section: Experimental Procedures 21 Radioactivity Measurementsmentioning

confidence: 99%

Radioactive Chernobyl Environment Has Produced High-Oil Flax Seeds That Show Proteome Alterations Related to Carbon Metabolism during Seed Development

et al. 2013

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Starting in 2007, we have grown soybean (Glycine max [L.] Merr. variety Soniachna) and flax (Linum usitatissimum, L. variety Kyivskyi) in the radio-contaminated Chernobyl area and analyzed the seed proteomes. In the second-generation flax seeds, we detected a 12% increase in oil content. To characterize the bases for this increase, seed development has been studied. Flax seeds were harvested in biological triplicate at 2, 4, and 6 weeks after flowering and at maturity from plants grown in nonradioactive and radio-contaminated plots in the Chernobyl area for two generations. Quantitative proteomic analyses based on 2-D gel electrophoresis (2-DE) allowed us to establish developmental profiles for 199 2-DE spots in both plots, out of which 79 were reliably identified by tandem mass spectrometry. The data suggest a statistically significant increased abundance of proteins associated with pyruvate biosynthesis via cytoplasmic glycolysis, L-malate decarboxylation, isocitrate dehydrogenation, and ethanol oxidation to acetaldehyde in early stages of seed development. This was followed by statistically significant increased abundance of ketoacyl-[acylcarrier protein] synthase I related to condensation of malonyl-ACP with elongating fatty acid chains. On the basis of these and previous data, we propose a preliminary model for plant adaptation to growth in a radio-contaminated environment. One aspect of the model suggests that changes in carbon assimilation and fatty acid biosynthesis are an integral part of plant adaptation.

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Section: Experimental Procedures 21 Radioactivity Measurementsmentioning

confidence: 99%