A TaqMan real-time PCR assay for quantifying white spot syndrome virus (WSSV) infections in wild broodstock and hatchery-reared postlarvae of fleshy shrimp, Fenneropenaeus chinensis

Jang, In‐Kwon; Meng, Xianhong; Seo, Hyung-Chul; Cho, Yeong-Rok; Kim, Bong-Rae; Gopalakannan, Ayyaru; Kim, Jong-Sheek

doi:10.1016/j.aquaculture.2008.10.038

Cited by 51 publications

(45 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This finding indicates a high prevalence of the virus among wild L. aestiva populations and is consistent with reports of high incidences of viral infections in other marine arthropods, including penaeid shrimp (34)(35)(36)(37). Currently, quantitative data on DNA viruses in marine arthropods are sparse.…”

Section: Atcopcv Lacopcvsupporting

confidence: 69%

“…Most of the research on this topic to date has involved white spot syndrome virus (WSSV) in crustaceans. The average viral load for LaCopCV is on the same order of magnitude as estimated WSSV loads in postlarvae of Fenneropenaeus chinensis (34), and approximately one order of magnitude higher than WSSV loads in Portunuus trituberculatus larvae obtained from diverse locations in Korea (35). The range of LaCopCV viral loads observed is similar to ranges reported in surveys of WSSV among P. trituberculatus larvae (35).…”

Section: Atcopcv Lacopcvsupporting

confidence: 69%

“…However, previous studies have focused on virus pathology, and few estimates of viral load or prevalence for marine crustacea have been published. To examine the viral load of LaCopCV within individual animals, we applied a quantitative PCR (qPCR) approach used previously to study viral loads in larvae of aquacultured prawns (34,35). qPCR of the putative capsid gene of LaCopCV revealed viral loads ranging from 77 to 7.75 × 10 5 copies per L. aestiva individual, with an average viral load of 1.13 × 10 5 copies per individual copepod and 100% of L. aestiva individuals infected in three of the four Fig.…”

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

Molecular and microscopic evidence of viruses in marine copepods

Dunlap

Rosario

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

As dominant members of marine mesozooplankton communities, copepods play critical roles in oceanic food webs and biogeochemical cycling. Despite the ecological significance of copepods, little is known regarding the causes of copepod mortality, and up to 35% of total copepod mortality cannot be accounted for by predation alone. Viruses have been established as ecologically important infectious agents in the oceans; however, viral infection has not been investigated in mesozooplankton communities. Here we used molecular and microscopic techniques to document viral infection in natural populations of the calanoid copepods Acartia tonsa (Dana) and Labidocera aestiva (Wheeler) in Tampa Bay, FL. Viral metagenomics revealed previously undocumented viruses in each species, named Acartia tonsa copepod circo-like virus (AtCopCV) and Labidocera aestiva copepod circo-like virus (LaCopCV). LaCopCV was found to be extremely prevalent and abundant in L. aestiva populations, with up to 100% prevalence in some samples and average viral loads of 1.13 × 10 5 copies per individual. LaCopCV transcription was also detected in the majority of L. aestiva individuals, indicating viral activity. AtCopCV was sporadically detected in A. tonsa populations year-round, suggesting temporal variability in viral infection dynamics. Finally, virus-like particles of unknown identity were observed in the connective tissues of A. tonsa and L. aestiva by transmission electron microscopy, demonstrating that viruses were actively proliferating in copepod connective tissue as opposed to infecting gut contents, parasites, or symbionts. Taken together, these results provide strong independent lines of evidence for active viral infection in dominant copepod species, indicating that viruses may significantly influence mesozooplankton ecology.

show abstract

Section: Atcopcv Lacopcvsupporting

confidence: 69%

Section: Atcopcv Lacopcvsupporting

confidence: 69%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Molecular and microscopic evidence of viruses in marine copepods

Dunlap

Rosario

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

“…They suggested that the WSSV DNA copies in shrimp treated with vp28-siRNA were significantly decreased (P < 0.01) by comparison with that of the positive control (WSSV only). Jang et al [76] have quantified the rate of WSSV infection in hatchery-reared post larvae of fleshy shrimp, Fenneropenaeus chinensis through TaqMan real-time PCR assay method. They observed that the post larvae collected from 7 hatcheries showed a range of 2.6e713.6 WSSV DNA copies ng À1 .…”

Section: Concentration Of Fucoidan (%)mentioning

confidence: 99%

The effect of fucoidan from brown seaweed Sargassum wightii on WSSV resistance and immune activity in shrimp Penaeus monodon (Fab)

Immanuel

Sivagnanavelmurugan

Marudhupandi

et al. 2012

Fish & Shellfish Immunology

154

View full text Add to dashboard Cite

“…The amplification programme consisted of 40 cycles of denaturation at 95°C for 30 s, annealing at 60°C for 30 s and extension at 72°C for 30 s. A standard curve was obtained using serial dilutions of plasmid pVP28 (full‐length ORF of VP28 gene of WSSV was cloned into pRSETB vector) that were used to quantify the WSSV viral genomic copy number. Each assay was carried out in triplicate (Jang et al., ; Musthaq & Kwang, ). Control animals were inoculated with tissue suspension prepared from uninfected shrimp.…”

Section: Methodsmentioning

confidence: 99%

A new strain of white spot syndrome virus affecting Litopenaeus vannamei in Indian shrimp farms

Sivakumar¹,

Vimal²,

Majeed³

et al. 2018

Journal of Fish Diseases

View full text Add to dashboard Cite

White spot syndrome virus (WSSV)-infected shrimp samples collected from grow-out ponds located at Nellore, Andhra Pradesh, India, showed WSSV negative and positive by PCR using primer sets specific to ORF119 and VP28 gene of WSSV, respectively. This indicated the deletion of genetic fragments in the genome of WSSV. The WSSV isolate along with lab strain of WSSV was subjected to next-generation sequencing. The sequence analysis revealed a deletion of 13,170 bp at five positions in the genome of WSSV-NS (new strain) relative to WSSV-TH and WSSV-LS (lab strain). The PCR analysis using the ORF's specific primer sets revealed the complete deletion of 10 ORFs in the genome of WSSV-NS strain. The primer set was designed based on sequence covering ORF161/162/163 to amplify a product of 2,748 bp for WSSV-LS and 402 bp for WSSV-NS. Our surveillance programme carried out since 2002 revealed the replacement of WSSV-LS by WSSV-NS in Indian shrimp culture system.

show abstract

A TaqMan real-time PCR assay for quantifying white spot syndrome virus (WSSV) infections in wild broodstock and hatchery-reared postlarvae of fleshy shrimp, Fenneropenaeus chinensis

Cited by 51 publications

References 20 publications

Molecular and microscopic evidence of viruses in marine copepods

Molecular and microscopic evidence of viruses in marine copepods

The effect of fucoidan from brown seaweed Sargassum wightii on WSSV resistance and immune activity in shrimp Penaeus monodon (Fab)

A new strain of white spot syndrome virus affecting Litopenaeus vannamei in Indian shrimp farms

Contact Info

Product

Resources

About