A tapping mode AFM study of collapse and denaturation in dentinal collagen

Feninat, F. El; Ellis, T. H.; Sacher, E.; Stangel, I.

doi:10.1016/s0109-5641(00)00083-x

Cited by 63 publications

(36 citation statements)

References 18 publications

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“…Literally, this leaves a collagen scaffold suspended in water [2]. The exposed collagen acts like a mesh with nanometric-size porosities of about 10-40 nm, that should be able to mechanically retain the fluid resin upon in situ polymerization [2,[44][45][46]. In fact, demineralized dentin becomes a highly hydrophilic substrate of difficult clinical manipulation regarding adhesive procedures, as these materials are extremely sensitive to the presence of water [12,33,[47][48][49].…”

Section: Etch-and-rinse Adhesive Systemsmentioning

confidence: 99%

Adhesion in Restorative Dentistry

Vinagre¹,

Ramos²

2016

Adhesives - Applications and Properties

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Bonding agents play a crucial role in the effective sealing and retention of resin-based composite restorations, which have been increasingly placed and replaced by dentists in many countries around the world. In fact, direct adhesive restoration with composite resins has become the procedure of choice for the treatment of anterior and posterior teeth. However, long-term durability of those restorations may be compromised due to progressive loss of the integrity of adhesive interfaces. This means that no adhesive strategy is free from technique sensitivity. The specificity and proportion of different constitutive molecules, the interaction between them and substrates can differ greatly from one class of adhesive system to another, which can affect bond quality. Protocol simplification has been an inevitable trend that has boosted the use of self-etching and universal systems in adhesive dentistry. However, there is a lack of randomized clinical trials to prove the effectiveness of these systems. This chapter gives an overview of the most important issues in dental adhesion and adhesive systems, as well, discussing their composition and clinical use.

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Section: Etch-and-rinse Adhesive Systemsmentioning

confidence: 99%

Adhesion in Restorative Dentistry

Vinagre¹,

Ramos²

2016

Adhesives - Applications and Properties

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“…Leakage of pulpal fluids from the tubules under hydrostatic pressure may disrupt attempts at chemical and micromechanical bonding to the dentin substrate. Early attempts to achieve bonding to dentin by extending the enamel acid-etching technique were not successful because air-drying the cavity caused the acid-etched dentin to collapse (29). Because mechanical bonding to etched dentin is not a viable alternative, research has instead focused on forming some type of chemical bond to hydroxyapatite and the collagen fibrils of dentin.…”

Section: Acid Etchingmentioning

confidence: 99%

Important compositional characteristics in the clinical use of adhesive systems

et al. 2014

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Improvements in dentin bonding systems have influenced modern restorative dentistry. The desire for minimal invasiveness has resulted in moreconservative cavity design, which basically relies on the effectiveness of current dentin bonding systems. Interaction of adhesives with enamel and dentin is based on two systems, commonly described as etchand-rinse and self-etch. Priming and bonding agents can be separate or combined, resulting in two-or three-step systems for etch-and-rinse adhesives and one-or two-step systems for self-etch adhesives. Selfetch systems use acidic functional monomers that simultaneously demineralize and impregnate tooth structures. Etch-and-rinse and self-etch systems have advantages and disadvantages, which are primarily related to the simplified bonding procedures required under clinical conditions. Knowledge of the composition, characteristics, and mechanisms of adhesion for each adhesive system is critical in selecting the ideal adhesive materials for clinical use. (J Oral Sci 56, 1-9, 2014)

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“…The dehydration process plays a crucial role in dental clinical applications because surface structural changes (caused by water-loss) can significantly affect dentin bonding (e.g., [46]). Current AFMs enable the imaging of structural changes in dentin collagen before and after dehydration, however, at current imaging speeds, the AFM cannot be used to study the rapid dehydration process [5]. This interest in high-speed AFM imaging of collagen motivates the use of collagen samples in the following experiments.…”

Section: The Need For High-speed Imaging Of Collagen Samplesmentioning

confidence: 99%

Control Issues in High‐speed Afm for Biological Applications: Collagen Imaging Example

Zou

Leang

Sadoun

et al. 2004

Asian Journal of Control

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This article considers the precision positioning problem associated with high-speed operation of the Atomic Force Microscope (AFM), and presents an inversion-based control approach to achieve precision positioning. Although AFMs have high (nanoscale) spatial resolution, a problem with current AFM systems is that they have low temporal resolution, i.e., AFM imaging is slow. In particular, current AFM imaging cannot be used to provide three-dimensional, time-lapse images of fast processes when imaging relatively-large, soft samples. For instance, current AFM imaging of living cells takes 1-2 minutes (per image frame) -such imaging speeds are too slow to study rapid biological processes that occur in seconds, e.g., to investigate the rapid movement of cells or the fast dehydration and denaturation of collagen. This inability, to rapidly image fast biological processes, motivates our current research to increase the operating speed of the AFM. We apply an inversionbased feedback/feedforward control approach to overcome positioning problems that limit the operating speed of current AFM systems. The efficacy of the method, to achieve high-speed AFM operation, is experimentally evaluated by applying it to image collagen samples.

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A tapping mode AFM study of collapse and denaturation in dentinal collagen

Cited by 63 publications

References 18 publications

Adhesion in Restorative Dentistry

Adhesion in Restorative Dentistry

Important compositional characteristics in the clinical use of adhesive systems

Control Issues in High‐speed Afm for Biological Applications: Collagen Imaging Example

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