Cell Reports volume 3, issue 6, P2155-2167 2013 DOI: 10.1016/j.celrep.2013.05.004 View full text
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Guillaume Diss, Alexandre K. Dubé, Joël Boutin, Isabelle Gagnon-Arsenault, Christian R. Landry

Abstract: Cells contain many important protein complexes involved in performing and regulating structural, metabolic, and signaling functions. One major challenge in cell biology is to elucidate the organization and mechanisms of robustness of these complexes in vivo. We developed a systematic approach to study structural dependencies within complexes in living cells by deleting subunits and measuring pairwise interactions among other components. We used our methodology to perturb two conserved eukaryotic complexes: the…

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