A survey on canine leishmaniasis in western Turkey by parasite, DNA and antibody detection assays

Özbel, Yusuf; Oskam, Linda; Ozensoy, S; Turgay, Nevın; Alkan, Mustafa; Jaffe, Charles L.; Özcel, M. A.

doi:10.1016/s0001-706x(99)00047-9

Cited by 56 publications

(40 citation statements)

References 14 publications

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“…The assay had been previously employed for serum diagnosis of canine infection by Leishmania in animals with asymptomatic visceral leishmaniasis (36,37). In the current study, the authors are reporting the presence of anti-Leishmania antibodies in Cerdocyon thous, Lycalopex vetulus, Cebus apella, Dasyprocta azarae, Procyon cancrivorus and Nasua nasua.…”

Section: Search For Antibodies By Datmentioning

confidence: 82%

Serological survey for Leishmania sp. infection in wild animals from the municipality of Maringá, Paraná state, Brazil

Voltarelli

Arraes

Perles

et al. 2009

J. Venom. Anim. Toxins incl. Trop. Dis

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Leishmania sp. infection was investigated in wild animals from the Ingá Park, in the municipality of Maringá, Paraná state, Brazil, where American cutaneous leishmaniasis (ACL) is an endemic disease. Sixty-five mammals, comprising Didelphis albiventris, Cerdocyon thous, Lycalopex vetulus, Cebus apella, Dasyprocta azarae, Dasypus novemcinctus, Procyon cancrivorus and Nasua nasua, were captured. Blood samples were collected for parasite cultivation. Antibodies were investigated by direct agglutination test (DAT) using Leishmania (Viannia) braziliensis as antigen. Flagellates were observed in blood cultures of 14 (35.9%) Didelphis albiventris. Anti-Leishmania antibodies were detected in 31 (51.6%) specimens of Cerdocyon thous, Lycalopex vetulus, Cebus apella, Dasyprocta azarae, Procyon cancrivorus and Nasua nasua. These results suggest that Cerdocyon thous and Lycalopex vetulus (crab-eating fox), Cebus apella (capuchin monkey), Dasyprocta azarae (agouti), Procyon cancrivorus (crab-eating raccoon) and Nasua nasua (coati) play an important role in the ACL transmission cycle in the northwestern region of Paraná, Brazil.

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Section: Search For Antibodies By Datmentioning

confidence: 82%

Serological survey for Leishmania sp. infection in wild animals from the municipality of Maringá, Paraná state, Brazil

Voltarelli

Arraes

Perles

et al. 2009

J. Venom. Anim. Toxins incl. Trop. Dis

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“…Seropositivity to Leishmania in dogs was reported 4.3 % in Bursa, 33.3% in Muğla (Coskun et al, 1997), 1% (2/50) in Kırıkkale (Aydenizöz et al, 2010), 7.95% (14/176) in Antalya ), 20.7 % in Denizli (Ozensoy Töz et al, 2009) and 2.58 % (3/116) in Ankara (Aslantaş et al, 2005). In addition, 5.3 % of 490 dogs in Manisa, (Ozbel et al, 2000), 3.2 % of 158 dogs in Izmir and Aydin (Voyvoda et al, 2004) and 9.1% of 109 dogs and 4.7% of 85 dogs in Kusadasi (Ozensoy et al, 2005) were found to be seropositive. Antibodies to Lesihmania in 13.51% of 111 dogs from Eskisehir, Afyon and Bilecik (Dogan et al, 2005), 5.9% of 432 dogs from Istanbul, Bursa and Çorlu (Uysal et al, 2003) and 3.07% of 65 dogs in Kocaeli (Tamer et al, 2008) were also detected.…”

Section: Discussionmentioning

confidence: 99%

“…Beside serological studies, there are few studies on direct detection of Leishmania in dogs, either by using antigen or DNA detection assays in Turkey. In one study (Ozbel et al, 2000), PCR was used to confirm leishmaniosis in seropositive dogs and the confirmation rate was found to be 13 (76.4%) in 17 seropositive dogs. A more recent study conducted in Kayseri province based on PCR detection of parasite revealed that none of the 300 dogs examined were positive for leishmaniosis (İça et al, 2008).…”

Section: Conflict Of Interest Statementmentioning

confidence: 99%

Prevalence of leishmaniosis in dogs in Istanbul,Turkey determined by using PCR

Bilgin

Turan

Yılmaz

et al. 2018

J Hellenic Vet Med Soc

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ABSTRACT. Leishmania infantum is widespread in Mediterranean countries including Turkey and can cause a serious disease in both humans and dogs. Dogs are considered to be its main reservoirs. The current epidemiological study was carried out in Istanbul for detection of leishmaniosis among dogs. A total of 246 dogs were included in the study. Twenty one (8.54%) blood samples were found to be positive based on PCR diagnosis, using primers specific for the kinetoplast DNA of Leishmania. Infection rate was 6.51% in 169 dogs living in shelters and 12.99% in 77 client-owned dogs. The rate was significantly (p<0.001) higher in the dogs (37.93%) presenting one or more clinical symptoms which may be attributable to leishmaniosis, than in the asymptomatic dogs (4.61%). Eleven (52.4%) of 21 PCR positive dogs presented clinical symptoms whereas the rest of the dogs (47.62%) were asymptomatic. The major clinical sign in PCR positive animals was dermatological problems. Amongst PCR positive dogs, skin lesions were present in 11, weight loss in 2, lymphadenopathy in 2, epistaxis in 2 and lethargy in 2 dogs.

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“…Hastalık dünyanın pek çok yerinde visseral leishmaniasis (VL), kutanöz leishmaniasis (KL), diffuz deri leishmaniasis (DKL) ve mukokutanöz leishmaniasis (MKL) gibi değişik formları görülmesine rağmen (3), Türkiye'de şimdiye kadar VL, KL ve köpek visseral leishmaniasis (KVL) formları görülmüştür (25). Hastalık Türkiye'de, bölgelerin iklim durumuyla yakından ilişkili olarak genellikle Akdeniz ve Ege bölgelerinden bildirilmiştir (1,7,10,26,27).…”

Section: Discussionunclassified

Determination the Role of Rhipicephalus sanguineus for Transmission of Leishmania major to Reservoir Animals

Bilgiç

Bakırcı²,

Köse³

et al. 2017

TurkiyeParazitolDerg

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üzerinde beslenmesini takiben, doymuş larvalar ve uygun koşullarda gömlek değiştiren larvalardan elde edilen aç nimflerden oluşan toplam 65 kene havuzu oluşturulmuştur. Bu havuzlar polimeraz zincir reaksiyonu (PZR) ve gerçek zamanlı PZR (RT-PCR) testi ile L. major yönünden test edilmiştir. Bulgular: Enfekte edilerek, üzerine Rh. sanguineus larvası konulan gerbil, tüm kenelerin düşmesini takiben uyutulmuş ve nekropsisi gerçekleştirilmiştir. Yapılan incelemelerde, gerbilin tüm iç organ ve dokularında amastigotlara rastlanmıştır. Rh. sanguineus doymuş larvalarının beslenmeleri esnasında paraziti alıp almadıkları ve bir sonraki nimf aşamasına aktarıp aktarmadıklarının test edilmesi amacıyla yapılan PZR ve RT-PCR sonucunda kene havuzlarının hiçbirisinde L. major'a rastlanılmamıştır. Sonuç: Çalışma neticesinde her ne kadar kenelerde etkenler tespit edilemese de, leishmaniasis ile ilişkili deneysel çalışmalarda asıl rezervuar olan köpeklerin kullanılması vektörlük potansiyeli yüksek olan insekt ve akarlar için daha net sonuçlar verebilecektir. Anahtar Kelimeler: Gerbil, Leishmania major, nakil, Rhipicephalus sanguineus Geliş Tarihi: 24.05.2016Kabul Tarihi: 06.10.2016 ABSTRACTObjective: This study aimed to explore the role of Rhipicephalus sanguineus in the transmission of Leishmania major, the etiological agent of zoonotic cutaneous leishmaniasis. Methods: Ten gerbils (Meriones unguiculatus) were infected with promastigotes of L. major, and 10 gerbils were maintained as controls. In a controlled environment, 2000 R. sanguineus larvae were fed to two gerbils. Following feeding to gerbils, 65 tick pools were prepared from the engorged larvae and molted unfed nymphs. These pools were tested for the presence of L. major using polymerase chain reaction and real time (RT) PCR. Results: One of the infected gerbil was anesthetized and necropsied following the dropping of all fed larvae. Following the examination, amastigotes were detected in all organs and tissues. PCR and RT-PCR were performed to test whether the engorged R. sanguineus larvae successfully took the parasite while feeding and was able to transmit it to the next nymphal stage; however, none of the tick pools were found to be positive for L. major. Conclusion: Although L.major was not detected in ticks that fed on gerbils, using dogs in experimental studies related to leishmaniasis will give clearer results in terms of detecting the potential role of insects and acars.

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A survey on canine leishmaniasis in western Turkey by parasite, DNA and antibody detection assays

Cited by 56 publications

References 14 publications

Serological survey for Leishmania sp. infection in wild animals from the municipality of Maringá, Paraná state, Brazil

Serological survey for Leishmania sp. infection in wild animals from the municipality of Maringá, Paraná state, Brazil

Prevalence of leishmaniosis in dogs in Istanbul,Turkey determined by using PCR

Determination the Role of Rhipicephalus sanguineus for Transmission of Leishmania major to Reservoir Animals

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