A survey of whole-plant corn silages from Minas Gerais dairy farms

Santos, Andréia O.; Junior, Gilson Sebastião Dias; Pereira, Marcos Neves; Schwan, Rosane Freitas; Ávila, Carla Luiza da Silva

doi:10.1590/1678-992x-2018-0080

Cited by 3 publications

(3 citation statements)

References 35 publications

(45 reference statements)

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“…Data regarding the production of metabolites by strains were analysed using principal component analysis (PCA) using the XL Stat software, version 7.5. The CORR procedure of SAS 9.3 (SAS Institute 2012) was used to analyses the correlation between the data of silage characteristics as dry matter (DM), pH, lactic, acetic, propionic and butyric acids, ethanol, 1,2propanediol and storage time (Santos et al 2020) and LAB species identified in the silages.…”

Section: Discussionmentioning

confidence: 99%

“…For the bacterial isolation experiment, silage samples were selected according to fermentative characteristics of pH and temperature. The total count of yeast, filamentous fungi and bacteria (Enterobacteriaceae and aerobic spore-forming bacteria) were also taken into consideration Santos et al 2020). In this sense, two samples from each region, one of the farms that showed overall the best (the lowest pH, silage temperature, Enterobacteriaceae, aerobic spore-forming bacteria and yeast and filamentous fungi population) and one of the farm that showed the worst characteristics (opposite to the previously mentioned) were selected, making a total of 18 samples.…”

Section: Sampling and Bacterial Isolationmentioning

confidence: 99%

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Lactic acid bacteria diversity in corn silage produced in Minas Gerais (Brazil)

et al. 2019

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Purpose The diversity of lactic acid bacteria (LAB) in silages produced in warm climate countries is not well known. This study aimed to identify and characterise the metabolic and genotypic aspects of autochthonous LAB isolated from corn silage produced in the state of Minas Gerais, Brazil. Methods Eighty-eight LAB were isolated. To evaluate their performance at the strain level, all isolates were distinguished among strains using random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) and repetitive extragenic palindromic PCR (REP-PCR) techniques. The organic acid and ethanol production were determined by high-performance liquid chromatography (HPLC). Result The fingerprints obtained by RAPD-PCR with a M13 primer were more discriminatory than those obtained with the REP-PCR technique using a (GACA)4 primer. Moreover, 28 representative isolates were identified as Lactobacillus acidophilus, L. buchneri, L. casei, L. diolivorans, L. hilgardii, L. paracasei, L. parafarraginis, L. plantarum, L. rhamnosus, L. zeae and Pediococcus acidilactici. Different fingerprinting profiles between isolates within the same species were observed. However, some strains isolated from different silages showed the same band profile, thus suggesting the presence of clusters with high similar fingerprints in silages from various regions. Conclusion A variation in LAB diversity was observed in the silages of the evaluated regions, with L. rhamnosus and L. buchneri showing the highest distribution. Differences in organic acid production were observed among the strains belonging to the same species. This research contributes to a better understanding of the LAB community present in corn silage produced in warm climates. These strains will be studied as potential silage starters.

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Section: Discussionmentioning

confidence: 99%

Section: Sampling and Bacterial Isolationmentioning

confidence: 99%

Lactic acid bacteria diversity in corn silage produced in Minas Gerais (Brazil)

et al. 2019

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“…From a microbiological viewpoint, the efficacy of these commercial inoculants, either as single or as multi-species formulae, has been generally assessed in controlled laboratory conditions that do not mimic the various management practices and changing environmental factors observed in large-scale ensiling (Muck and Shinners, 2001;Kraut-Cohen et al, 2016;Ni et al, 2017). Consequently, few studies have evaluated the microbial communities populating silage stored in farm-scale silo types (Kraut-Cohen et al, 2016;Dos Santos et al, 2020), and none of this kind has focused on associations between physicochemical characteristics and the viable microbiota at feed-out, while contrasting uninoculated versus inoculated silage. Gagnon et al (2020) recently used a culture-based approach to analyze LAB communities occurring in hay and grass/legume and corn silage produced with and without inoculation at selected dairy farms.…”

Section: Introductionmentioning

confidence: 99%

Metataxonomic insights into the microbial ecology of farm-scale hay, grass or legume, and corn silage produced with and without inoculants

et al. 2022

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The microbiota of silage is a key determinant of its quality. Although commercial inoculants are often used to improve silage quality, studies to analyze their impact on the microbiota of preserved forage at farm-scale facilities are scarce. We assessed the diversity of viable bacterial communities of hay (unfermented dry forage) and grass or legume (GL) and corn (C) silage to deepen our knowledge of how inoculant addition drives microbial occurrence patterns on dairy farms. Forage samples were collected from 24 dairy farms over two sampling periods. Samples were analyzed by high-throughput sequencing and quantitative PCR after being treated with propidium monoazide to account for viable cells. We found consistent significant differences between hay and silage community structures across sampling periods. Silage was generally dominated by lactic acid bacteria (LAB), while Pantoea and Sphingomonas were the main co-dominant genera in hay. The GL silage dominated by Pediococcus, Weissella, and Bacillus was phylogenetically different from C silage enriched in Acetobacter. The use of inoculants including Lentilactobacillus buchneri either alone or in combination with Lactiplantibacillus plantarum, Lacticaseibacillus casei, Pediococcus pentosaceus, or Enterococcus faecium did not systematically prevent the occurrence of undesirable bacteria, especially when corn-based, probably because of factors that can mitigate the effect of inoculation on the microbiota. The core Lactobacillales constituted the dominant LAB in silage with up to 96% relative abundance, indicating either the ubiquity of inoculants or the high competitiveness of epiphytes. Silage chemical profiles varied inconsistently with sampling periods and the use of inoculants. Multivariate multi-table analyses allowed the identification of bacterial clusters mainly driven by moisture and magnesium content in hay, while pH, lactic, and fatty acids were the main drivers for silage. Bacterial network analyses showed considerable variations in the topological roles with the use of inoculants. These results may help evaluate the effectiveness of forage management practices implemented on dairy farms and, therefore, are useful for fine-tuning the search for new additives. Such knowledge can be used by forage makers to adjust processing routines to improve the hygienic quality, nutritional potential, and aerobic stability of preserved forage.

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A survey of whole-plant corn silages from Minas Gerais dairy farms

Cited by 3 publications

References 35 publications

Lactic acid bacteria diversity in corn silage produced in Minas Gerais (Brazil)

Lactic acid bacteria diversity in corn silage produced in Minas Gerais (Brazil)

Metataxonomic insights into the microbial ecology of farm-scale hay, grass or legume, and corn silage produced with and without inoculants

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