A survey of symbiotic nitrogen fixation by white clover grown on metal contaminated soils

Broos, Kris; Uyttebroek, Maarten; Mertens, Jean–François; Smolders, Erik

doi:10.1016/j.soilbio.2003.11.007

Cited by 75 publications

(50 citation statements)

References 19 publications

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“…Our results clearly indicate that the Met Rh strain isolated from a metallicolous A. vulneraria nodule is a metal-tolerant bacterium. Moreover, the metal concentration in the mine spoils at Les Avinières (Table 1) is far higher than that of sludgeamended soils where the effects of metal toxicity on symbiotic nitrogen fixation have previously been studied (Obbard and Jones 2001;Broos et al 2004) and consequently Met Rh exhibited a remarkably high Zn tolerance level.…”

Section: Rhizobium Survival In Heavy Metal-contaminated Soilmentioning

confidence: 93%

“…The survival of a healthy population of the microsymbiont appears to be the most critical factor influencing N fixation (Broos et al 2004(Broos et al , 2005. In other studies, Castro et al (1997) showed that only 15% of a Rhizobium leguminosarum cv.…”

Section: Rhizobium Survival In Heavy Metal-contaminated Soilmentioning

confidence: 98%

“…Thus, an inhibition of nitrogen fixation has been observed at moderate zinc concentrations in soils, for example 366 mg Zn kg -1 for broad bean (Vicea faba) and pea (Pisum sativum) inoculated with their symbiont Rhizobium leguminosarum biovar. vicea (Obbard and Jones 2001) and 614 mg Zn kg -1 for non-inoculated white clover (Trifolium repens) (Broos et al 2004). Nodulation of broad bean and pea totally failed at 664 mg Zn kg -1 soil (Obbard and Jones 2001).…”

Section: Rhizobium Survival In Heavy Metal-contaminated Soilmentioning

confidence: 99%

“…Most of the measurements of symbiotic N 2 fixation in heavy metal-contaminated soils have concerned cultivated leguminous plants growing in soils amended with sewage sludge where heavy metal concentrations were far lower than those encountered in mine tailings (Obbard and Jones 2001;Broos et al 2004;Chaudri et al 2008).…”

Section: Introductionmentioning

confidence: 99%

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Anthyllis vulneraria/Mesorhizobium metallidurans, an efficient symbiotic nitrogen fixing association able to grow in mine tailings highly contaminated by Zn, Pb and Cd

Mahieu

Frérot

Vidal³

et al. 2011

Plant Soil

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The excessive concentrations of toxic heavy metals in mine tailings and their very low N content make soil reclamation strategies by phytostabilization difficult. Our objective was to test if the symbiotic association between the legume Anthyllis vulneraria subsp. carpatica and the bacteria Mesorhizobium metallidurans originating from highly polluted mine tailings is able to increase N concentration in soils with contrasting Zn, Pb and Cd contents. Plants of A. vulneraria subsp. carpatica from a mine site and of a non-metallicolous subsp. praeopera from non-polluted soil were inoculated with a metallicolous or a non-metallicolous compatible Mesorhizobium spp. and grown on low and high heavy metal-contaminated soils. In contaminated soil, many nodules were observed when the metallicolous A. vulneraria was inoculated with its rhizobium species M. metallidurans, whereas the non-metallicolous A. vulneraria died after a few weeks regardless of the rhizobium inoculant. Eighty percent of the total nitrogen was derived from biological nitrogen fixation through the association between metallicolous A. vulneraria and the rhizobium grown on metal-enriched soil. The ability of the metallicolous A. vulneraria to develop a high nitrogen fixing potential opens new possibilities for promoting a low-maintenance plant cover and for stabilizing the vegetation in high heavy metal-contaminated soils

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Section: Rhizobium Survival In Heavy Metal-contaminated Soilmentioning

confidence: 93%

Section: Rhizobium Survival In Heavy Metal-contaminated Soilmentioning

confidence: 98%

Section: Rhizobium Survival In Heavy Metal-contaminated Soilmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Anthyllis vulneraria/Mesorhizobium metallidurans, an efficient symbiotic nitrogen fixing association able to grow in mine tailings highly contaminated by Zn, Pb and Cd

Mahieu

Frérot

Vidal³

et al. 2011

Plant Soil

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“…However, most of the studies on T. repens growing in metal-polluted areas have been focused on the symbiotic nitrogen fixation (Broos et al 2004;Castro et al 2003;Chaudri et al 2008), and little attention has been paid to the effect of PGPR on the growth of white clover in metal-contaminated soils. The objectives of this study were to test the ability of several PGPR previously isolated from a heavy metal-contaminated site in Estarreja, Northern Portugal, to produce plant growthpromoting substances, such as indole-3-acetic acid (IAA), NH 3 , HCN, siderophores and extracellular enzymes (lipases, cellulases, proteases, pectinases), to solubilize phosphate and ACC-deaminase activity and to assess the PGPR potential to promote Trifolium repens growth under axenic conditions.…”

Section: Introductionmentioning

confidence: 99%

Rhizobacteria isolated from a metal-polluted area enhance plant growth in zinc and cadmium-contaminated soil

Pereira

Barbosa

Castro

2014

Int. J. Environ. Sci. Technol.

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In this work, we evaluated the ability of rhizobacteria isolated from a metal-contaminated site to produce plant growth-promoting substances, such as indole-3-acetic acid (IAA), ammonia, hydrogen cyanide, siderophores and extracellular enzymes, to solubilize phosphate and for 1-aminocyclopropane-1-carboxylate (ACC)-deaminase activity, while assessing their capacity to enhance Trifolium repens growth. The rhizobacteria that better performed were used as bioinoculants for plants grown in zinc-and cadmium-spiked soils. Rhizobacteria were inoculated onto white clover seedlings growing in 250 and 500 mg kg -1 of zinc and in 10 and 30 mg kg -1 of cadmium-spiked soils and plant traits determined. The available and exchangeable rhizosphere metal concentrations were analyzed. Twenty rhizobacteria stimulated white clover growth under axenic conditions. Overall, strains Rhodococcus erythropolis EC 34, Achromobacter sp. 1AP2 and Microbacterium sp. 3ZP2 increased clover biomass in the absence and in presence of metals; however, in soils with 250 mg kg -1 of zinc, bacterial inoculation did not enhance plant biomass. Biomass enhancement may be related to the multiple bacterial promoting traits, especially the production of high levels of IAA and siderophores and the activity of ACC-deaminase. To the best of our knowledge, this is the first study reporting the potential of R. erythropolis to increase plant growth in metal-contaminated soils. In general, strains Microbacterium sp. 3ZP2 and Arthrobacter sp. EC 10 enhanced the available and exchangeable metal concentrations in rhizosphere showing their potential to improve phytoremediation efficiency. This work clearly indicates that rhizobacteria are promising for the development of bioinoculants useful for phytoremediation of polluted soils.

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Plant-Fungal Association: An Ideal Contrivance for Combating Plant Stress Tolerance

et al. 2021

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A survey of symbiotic nitrogen fixation by white clover grown on metal contaminated soils

Cited by 75 publications

References 19 publications

Anthyllis vulneraria/Mesorhizobium metallidurans, an efficient symbiotic nitrogen fixing association able to grow in mine tailings highly contaminated by Zn, Pb and Cd

Anthyllis vulneraria/Mesorhizobium metallidurans, an efficient symbiotic nitrogen fixing association able to grow in mine tailings highly contaminated by Zn, Pb and Cd

Rhizobacteria isolated from a metal-polluted area enhance plant growth in zinc and cadmium-contaminated soil

Plant-Fungal Association: An Ideal Contrivance for Combating Plant Stress Tolerance

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