A surface-scanning coil detector for real-time, in-situ detection of bacteria on fresh food surfaces

Chai, Yating; Horikawa, S.; Li, Suiqiong; Wikle, Howard C.; Chin, Bryan A.

doi:10.1016/j.bios.2013.06.056

Cited by 64 publications

(36 citation statements)

References 16 publications

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“…Recently, Chai et al developed a novel ME biosensor measurement technique using a surface scanning coil for practical applications [13][14]38 . In this new method, a planer coil was fabricated and placed closely above the ME sensor.…”

Section: Surface-scanning Coil Detector For Real-time In Situ Pathogementioning

confidence: 99%

“…Recently, magnetostrictive material based biosensors, especially free-standing magnetoelastic (ME) biosensors, have been investigated as a label-free wireless biosensor system for real-time pathogen detection [7][8][9][10][11][12][13][14] . A ME biosensor is comprised of a free-standing sensor platform made of a strip-shaped magnetoelastic particle and a biomolecular recognition element (antibody, phage, enzymes, etc.)…”

Section: Introductionmentioning

confidence: 99%

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High throughput pathogen screening for food safety using magnetoelastic biosensors

Chai

Chin

2015

SPIE Proceedings

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In order to secure food safety, high throughput pathogen screening technique that can quickly identify and isolate unsafe contaminated foods has been long-desired. Recently, magnetoelastic (ME) free-standing biosensors have been investigated as a label-free wireless biosensor system for real-time pathogen detection. ME biosensor is composed of a ME resonator coated with a bio-molecular recognition element that binds specifically with a target pathogen. Once the biosensor comes into contact with the target pathogen, binding occurs, resulting in a decrease of the sensor's resonant frequency. Interrogated through magnetic signals, large amount of ME sensors can be deployed and monitored wirelessly. ME biosensors have been investigated to detect foodborne pathogens in cultures and liquid foods. Recently, it has been demonstrated that phage-based ME biosensors are able to directly detect Salmonella Typhimurium on food surfaces without the requirement of pre-analysis culture preparation. This paper will review the novel ME biosensor technique, including the detection principle, the characterization of the sensor performance, the deployment of multiple sensor detection and their applications, especially for food safety analysis. The ME biosensor technique has the potential to be a powerful pathogen screening tool for detecting contaminated food, identifying critical hazard points, and tracking contamination sources along the entire food supply chain.

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Section: Surface-scanning Coil Detector For Real-time In Situ Pathogementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

High throughput pathogen screening for food safety using magnetoelastic biosensors

Chai

Chin

2015

SPIE Proceedings

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“…Figure 4B indicates that modified gold surface was successfully linked to the Au-SAM surface. Figure 4C are aggregates of the bacteriophage over the Au-SAM surface (Shabani et al, 2008;Garcıa-Gonzalez et al, 2008;Chai et al, 2013).…”

Section: Gold Surface Characterization By Semmentioning

confidence: 99%

Bacteriophage based self-assembled monolayer (SAM) on gold surface used for detection of Escherichia coli by electrochemical analysis

Singh¹,

Jain²,

Dahiya³

2015

Afr. J. Microbiol. Res.

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Bacteriophages (or phages) are parasites that infect specific bacteria. This host-selectivity can be useful to identify bacterial contaminants in food, water, environment etc. In the present study, phage was isolated from stagnant water and cultivated by overlay method against host bacteria that is E. coli. Phage titer was calculated to be 10 7 pfu/ml using 10-fold dilutions. Plaque reaction activity was observed within 4 to 6 h against host bacteria by spot test. Morphological identification of phage by transmission electron microscopy (TEM) using uranyl acetate staining revealed about 78 nanometers (nm) in wide phage capsid and tail length (527 nm). The isolated phage was classified into order Caudovirales since it possessed a long non-contractile tail and icosahedral capsid head, thus is a member of the family Siphoviridae. Also, the phage identified followed a lytic life cycle since plaque reaction activity was observed within 4-6 h against host bacteria. Gold immuno-functionalization using self-assembled monolayers (SAM) has been widely used for the detection of small targets, but there are limited reports available describing the detection systems for bacteria by using phages. Thus, in order to develop a suitable detection system for identification of specific bacteria, it is suitable to exploit the close association and selectivity between bacteria and bacteriophage. Bacteriophages were immobilized onto gold surface by SAM using a stable acyl amino ester intermediate generated by 1-ethyl-3(3-dimethylaminoproply) carbodiimide (EDC) and N-hydrosuccinimide (NHS) to condense the bacteriophage. Fourier transformation infrared (FTIR) microscopy presence of different functional groups present in each layer formation. Bacteriophage immobilization over the gold surface was verified through by scanning electron microscope (SEM). Electrochemical analysis was performed for a rapid and specific detection of E. coli cell. The present bio-sensing system comprises of quick and specific detection of host bacteria and possesses a very low detection limit (10 4 cfu/ml). We propose phages utilization as a bio-component in biosensor development for bacteria capture.

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“…In general, testing food for bacterial contamination requires sample preparation (e.g., washing, capturing, purification and concentration), since a liquid solution is required for typical biosensing techniques [2][3] . A revolutionary alternate to conventional pathogen detection methods is proposed that enables rapid, on-site detection of pathogenic bacteria on fresh produce without sample preparation and/or enrichment in the testing process 4 . The method used in this work relies on the combined use of (1) wireless phage-based ME biosensors and (2) a surface-scanning coil detector, enabling direct detection of multiple pathogens on various food surfaces.…”

Section: Introductionmentioning

confidence: 99%

In-situ detection of multiple pathogenic bacteria on food surfaces

Chai

Horikawa

et al. 2015

SPIE Proceedings

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Real-time in-situ detection of pathogenic bacteria on fresh food surfaces was accomplished with phage-based magnetoelastic (ME) biosensors. The ME biosensor is constructed of a small rectangular strip of ME material that is coated with a biomolecular recognition element (phage, antibodies or proteins, etc.) that is specific to the target pathogen. This mass-sensitive ME biosensor is wirelessly actuated into mechanical resonance by an externally applied time-varying magnetic field. When the biosensor binds with target bacteria, the mass of the sensor increases, resulting in a decrease in the sensor's resonant frequency. In order to compensate for nonspecific binding, control biosensors without phage were used in this experiment. In previous research, the biosensors were measured one by one. However, the simultaneous measurement of multiple sensors was accomplished in this research, and promises to greatly shorten the analysis time for bacterial detection. Additionally, the use of multiple biosensors enables the possibility of simultaneous detection of different pathogenic bacteria. This paper presents results of experiments in which multiple phage-based ME biosensors were simultaneously monitored. The E2 phage and JRB7 phage from a landscape phage library served as the bio-recognition element that have the capability of binding specifically with Salmonella typhimurium and B. anthracis spores, respectively. Real-time in-situ detection of Salmonella typhimurium and B. anthracis spores on food surfaces are presented.

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A surface-scanning coil detector for real-time, in-situ detection of bacteria on fresh food surfaces

Cited by 64 publications

References 16 publications

High throughput pathogen screening for food safety using magnetoelastic biosensors

High throughput pathogen screening for food safety using magnetoelastic biosensors

Bacteriophage based self-assembled monolayer (SAM) on gold surface used for detection of Escherichia coli by electrochemical analysis

In-situ detection of multiple pathogenic bacteria on food surfaces

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