A Surface Plasmon Resonance Biochip That Operates Both in the Angular and Wavelength Interrogation Modes

Abstract

This paper presents a surface plasmon resonance system based on a polymer prism chip. The device allows operation in both the angular and wavelength interrogation modes. The biochip design is discussed emphasizing the effect of the ambient temperature over the optical behavior. Birefringence effect, biochip polishing, and responsivity are also reported. The basic mathematical formulation for both operating modes is discussed, and morphological parameters are considered in the data analysis. Experimental sensorgrams obtained at both interrogation modes with the same polymer prism chip are presented and compared. The experimental sensorgrams obtained with assays providing reversible (phosphate buffered saline and hypochlorite solutions) and irreversible (neutravindin solution) bindings demonstrate the feasibility of the proposed design.

Polymer-based surface plasmon resonance biochip: construction and experimental aspects

“…To minimize this effect, the biochip prisms have heated until relaxation stress temperature, about 250°C for 24 hours, and controllably cooled to room temperature, in a process called annealing. The biochips without proper annealing do not exhibit the typical SPR resonance dip (Oliveira et al, 2013;Oliveira et al, 2014a). Also, the prism polishing improves the device performance by emphasizing the resonance dip and reducing transmission losses.…”

“…Such combinations aim at reducing the final cost of the sensor and also increase the number of possible operating points (OP), i.e., the pair for both the AIM and WIM modes (Oliveira et al, 2013;Oliveira et al, 2014b). Recently, palladium and other metals have been used to provide good characteristics for biological and industrial applications (Zuppella et al, 2015).…”

Filtro de dados baseado em rede neural artificial para remoção de ruídos da resposta de sensores SPR

“…Nasúltimas décadas sensores SPR tornaramse uma tecnologia madura, onde diversas estruturas foram propostas e desenvolvidas para melhorar a qualidade desse tipo sensor. A remoção de partes móveis presentes no sensor Oliveira et al (2013), o uso de fibrasópticas como guia de onda Sharma et al (2007), novos protocolos para funcionalização de camadas biológicas Bieri et al (1999) Sipova andHomola (2013) Ferreira et al (2017) e novas configurações eletrônicas com uso de smartphones Liu et al (2015) são exemplos de avanços que permitiram a obtenção de sensores de alta sensibilidade.…”

Fiber-Optic SPR Sensor for Temperature Measurement

“…D URING the last decade, surface plasmon resonance (SPR) sensors have been widely applied in chemical research [1] and biosensing [2]. SPR generally refers to the coupling between the electromagnetic wave and the surface plasmon wave (SPW), on the interface between a metal and a dielectric medium, and this coupling causes a decrease in the reflected intensity.…”