A Subset of Olfactory Sensory Neurons Express Forkhead Box J1-Driven eGFP

Abstract

250 words max, avoid references and abbreviations, unstructured) Forkhead box protein J1 (Foxj1), a member of the forkhead family transcription factors, is a transcriptional regulator of motile ciliogenesis. The nasal respiratory epithelium, but not olfactory epithelium, is lined with FOXJ1-expressing multiciliated epithelial cells with motile cilia. Using a Foxj1 -eGFP reporter mouse, we find robust eGFP expression not only in the multi-ciliated cells of the respiratory epithelium, but in a distinctive small subset of olfactory sensory neurons in the olfactory epithelium. These eGFP-positive cells lie at the extreme apical part of the neuronal layer and are most numerous in dorsal-medial regions of olfactory epithelium. Interestingly, we observed a corresponding small number of glomeruli in the olfactory bulb wherein eGFP-labeled axons terminate, suggesting that the population of eGFP+ receptor cells expresses a limited number of olfactory receptors. Similarly, a subset of vomeronasal sensory neurons express eGFP but these distribute throughout the full height of the vomeronasal sensory epithelium. In keeping with this broad distribution of labeled vomeronasal receptor cells, eGFP labeled axons terminate in many glomeruli of the accessory olfactory bulb. These findings suggest that Foxj1 -driven eGFP marks a specific population of olfactory and vomeronasal sensory neurons although neither receptor cell population possess motile cilia.

Differential Expression of Mucins in Murine Olfactory Versus Respiratory Epithelium

“…Nasal epithelia were obtained from 6, 2-to 4-month old sibling wild type (C57BL/6) mice, 2 OMP-ChR2-YFP (C57BL/6 background) mice (Li et al 2014), and 1 FOXJ1-eGFP mouse (Ostrowski et al 2003;Pathak et al 2019). Mice were kept in the National Institutes of Health-approved animal facility of the University of Colorado Anschutz Medical Center.…”