A stability-indicating HPLC assay method for docetaxel

Rao, B.M.; Chakraborty, Arpita; Srinivasu, M.K.; Devi, M. Lalitha; Kumar, Pramod; Chandrasekhar, K. B.; Srinivasan, Aloka; Prasad, Anand R.; Ramanatham, J.

doi:10.1016/j.jpba.2006.01.011

Cited by 66 publications

(38 citation statements)

References 3 publications

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“…The limit of detection (LOD) and quantification (LOQ) were found to be 36 and 156 ng/ml, respectively. Following formula was used to calculate the entrapment efficiency 16,[25][26][27][28] .…”

Section: Entrapment Efficiency (%Ee)mentioning

confidence: 99%

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Development of docetaxel nanocapsules for improvingin vitrocytotoxicity and cellular uptake in MCF-7 cells

Singh

Banala

Gupta

et al. 2015

Drug Development and Industrial Pharmacy

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The aim of this study was to fabricate docetaxel loaded nanocapsules (DTX-NCs) with a high payload using Layer-by-Layer (LbL) technique by successive coating with alternate layers of oppositely charged polyelectrolytes. Developed nanocapsules (NCs) were characterized in terms of morphology, particle size distribution, zeta potential (ζ-potential), entrapment efficiency and in vitro release. The morphological characteristics of the NCs were assessed using transmission electron microscopy (TEM) that revealed coating of polyelectrolytes around the surface of particles. The developed NCs successfully attained a submicron particle size while the ζ-potential of optimized NCs alternated between (+) 34.64 ± 1.5 mV to (-) 33.25 ± 2.1 mV with each coating step. The non-hemolytic potential of the NCs indicated the suitability of the developed formulation for intravenous administration. A comparative study indicated that the cytotoxicity of positively charged NCs (F4) was significant higher (p < 0.05) rather than negative charged NCs (F3), plain drug (DTX) and marketed preparation (Taxotere®) when evaluated in vitro on MCF-7 cells. Furthermore, cell uptake studies evidenced a higher uptake of positive NCs (≥1.2 fold) in comparison to negative NCs. In conclusion, formulated NCs are an ideal vehicle for passive targeting of drugs to tumor cells that may result in improved efficacy and reduced toxicity of encapsulated drug moiety.

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Section: Entrapment Efficiency (%Ee)mentioning

confidence: 99%

“…The release was carried out for 72 h and the samples were quantified by previously mentioned RP-HPLC method 26,28,29 .…”

Section: Entrapment Efficiency (%Ee)mentioning

confidence: 99%

Development of docetaxel nanocapsules for improvingin vitrocytotoxicity and cellular uptake in MCF-7 cells

Singh

Banala

Gupta

et al. 2015

Drug Development and Industrial Pharmacy

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“…Predetermined amount of sodium chloride was added to DT CLNC on a vortexer and the dispersion was centrifuged at 25 000 at 4 C on Ultracentrifuge Optima Max-XP (Beckman Coulter, Brea, CA). The pellet as well as clear dispersion medium were analyzed for DT content to determine encapsulation efficiency using HPLC method reported earlier with slight modifications 15,16 . DT was extracted from lipidic pellet using methanol before analysis.…”

Section: Drug Content and Encapsulation Efficiency Studiesmentioning

confidence: 99%

Docetaxel in cationic lipid nanocapsules for enhancedin vivoactivity

Jain

Makhija

Goel

et al. 2014

Pharmaceutical Development and Technology

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The usefulness of Docetaxel (DT) as an anti-cancer agent is limited to parenteral route owing to its very poor oral bioavailability. Thus, to improve its oral efficacy, DT was loaded in novel cationic lipid nanocapsules (DT CLNC). The DT CLNC possessed size of 130-150 nm, zeta potential of +72mV, adequate DT loading and over 95% encapsulation efficiency. TEM revealed capsular structure of DT CLNC. Lipolysis study indicated improved solubilization of DT by nanocapsules in comparison to DT solution. DT CLNC exhibited significantly higher release of DT in comparison to DT solution during in vitro permeation studies employing non-reverted rat-intestinal sac. Superior uptake of DT in zebra fishes exposed to DT CLNC resulted in greater apoptosis-based cell death as compared to those exposed to DT solution. This correlated well with the significantly superior (p < 0.05) anti-angiogenic activity of DT CLNC system over DT solution, in zebra fish model. DT CLNC also inhibited tumor growth in melanoma cell line induced tumors in C57BL/6 mice significantly, as compared to DT solution (p < 0.05). The DT CLNC system demonstrated adequate stability, with tremendous potential to improve oral efficacy of DT and can serve as an alternative to existing DT formulations available commercially for parenteral use.

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“…A validated HPLC method was developed to determine the stability of DTX by separating DTX from its degradation products (25,26). The release of DTX from the MC/AS/Pluronic F127 (denoted as gel/micelle combination system) was studied in the transwell system and the stability of released DTX was measured by the HPLC method.…”

Section: Stability and In Vitro Release Profile Of Dtx From Gel/micelmentioning

confidence: 99%

Low-Molecular-Weight Methylcellulose-Based Thermo-reversible Gel/Pluronic Micelle Combination System for Local and Sustained Docetaxel Delivery

et al. 2011

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A stability-indicating HPLC assay method for docetaxel

Cited by 66 publications

References 3 publications

Development of docetaxel nanocapsules for improvingin vitrocytotoxicity and cellular uptake in MCF-7 cells

Development of docetaxel nanocapsules for improvingin vitrocytotoxicity and cellular uptake in MCF-7 cells

Docetaxel in cationic lipid nanocapsules for enhancedin vivoactivity

Low-Molecular-Weight Methylcellulose-Based Thermo-reversible Gel/Pluronic Micelle Combination System for Local and Sustained Docetaxel Delivery

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