A Specific Inhibitor for Human Desoxyribonuclease and an Inhibitor of the Lupus Erythematosus Cell Phenomenon From Leucocytes 1

Abstract

In speculating upon the probable role of desoxyribonuclease (DNase) in the depolymerization of desoxyribonucleic acid (DNA), which characterizes (1) the "lupus erythematosus (L.E.) cell" (2), we have suggested (3), as have Klemperer and his associates (4), that an increased serum enzyme activity, potentiation of an intracellular DNase, or destruction of an intracellular inhibitor of DNase by a serum factor, might be responsible. In a previous communication (5) we reported evidence which excludes the serum DNase from responsibility for the phenomenon. Studies on the interaction of leucocytes and serum, in an approach to the alternative possibilities, disclosed the presence of a specific intracellular inhibitor of human serum DNase and an inhibitor of the "L.E.cell" phenomenon (6, 7). In the present paper, we shall report upon some of the characteristics of these factors.

Studies on Desoxyribonuclease in Systemic Lupus Erythematosus. Non-Participation of Serum Desoxyribonuclease in the “L.E. Phenomenon” 1

“…cell phenomenon, subsequent studies (28)(29)(30) suggest the participation of intracellular DNase and a DNase inhibitor in the depolymerization of DNA which characterizes this phenomenon. SUMMARY 1.…”

A Disorder of Blood Coagulation in Systemic Lupus Erythematosus 12

“…It is extremely difficult to quantitate L. E. cell factor activity with any degree of accuracy or reproducibility. To the extent to which this was possible (using previously published methods [5]), the L. E. cell-producing-potential of a serum bore no relationship to the anticoagulant activity of blood from the same patient.…”

Improved L.E. Cell Test Using Finger-prick Blood

“…Kurnick et al (1953) have discussed the probability of an L.E. cell phenomenon inhibitor, and it is possible we are dealing with such an inhibitor when failures occur.…”