A Sox5 gene is expressed in the myogenic lineage during trout embryonic development

Abstract

Sox proteins form a family of transcription factors characterized by the presence of a DNA-binding domain called the high-mobility-group domain (HMG). The presence of a large number of potential Sox5 binding sites in the trout promoter of Pax7, a gene which has emerged as an important regulator of neural and somite development, prompted us to clone trout Sox5 and to examine its expression pattern in the developing trout embryo. Using whole mount in situ hybridisation, we show here that the Sox5 transcript is first expressed before segmentation in the whole presomitic mesoderm. In newly formed somites, Sox5 labelling was observed in myogenic progenitor cells of the posterior and anterior walls. As the somite matured rostrocaudally, Sox5 expression disappeared from the differentiating embryonic myotome, deep in the somite, to become restricted to the undifferentiated myogenic precursors forming the dermomyotome-like epithelium at the surface of the embryonic myotome. Sox5 was also expressed in the developing nervous system and in pectoral fin buds. On the whole, this work suggests a hitherto unappreciated role for Sox5 in regulating myogenic cells destined to muscle formation and growth.

Gene expression profiling of the hyperplastic growth zones of the late trout embryo myotome using laser capture microdissection and microarray analysis

“…Sox5 and Sox11 transcripts were also up-regulated in laser-captured samples from the peripheral domain of the myotome. We have recently shown that Sox5 is expressed in myogenic cells from the dermomyotome-like epithelium notably at the level of the dorsal and ventral lips [26]. We report in this study a similar pattern of sox11 expression, indicating that these two genes are transcribed in dermomyotome-derived cells prior their differentiation.…”

What determines growth potential and juvenile quality of farmed fish species?

“…The MRFs act downstream of, or in parallel with, the paired domain and homeoboxcontaining transcription factors Pax 3 and Pax 7 (Fig. 2;Buckingham & Vincent 2009), with several Sox genes contributing to the control of muscle differentiation (Rescan & Ralliere 2010). The cells at the major horizontal septum retain a connection with the notochord and are recognized as muscle pioneer cells with a distinct programme of gene expression.…”

SOX5 promotes breast cancer proliferation and invasion by transactivation of EZH2

“…Sex determining region Y-box protein 5 (SOX5) is a member of the SOX family, which was identified based on the conserved homology of the high-mobility group DNA-binding motif (7). It has been reported that SOX5 is involved in the regulation of embryonic development (8), and is associated with various cancer types, including prostate cancer (9), glioblastoma (10), hepatocellular carcinoma (11), osteosarcoma (12) and nasopharyngeal carcinoma (13). In 2014, Pei et al (14) reported that in breast cancer, SOX5 induces epithelial-mesenchymal transition (EMT) by transactivation of Twist1 expression.…”