A small camel-milk protein rich in cysteine/half-cystine

Beg, Obaid Ullah; Bahr‐Lindström, Hedvig von; Zaidi, Zafar H.; Jörnvall, Hans

doi:10.1007/bf01116700

Cited by 28 publications

(22 citation statements)

References 5 publications

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“…Fractionation of camel milk whey proteins by exclusion chromatography on Sephadex G-100 [6] gives four fractions (numbered I-IV in fig. 1, [6]), which are purified by reverse-phase HPLC, using a gradient of acetonitrile in O.lolo trifluoroacetic acid [6] Sum 112 9 11 80 17 Results from acid hydrolysis for 24 h are given in molar ratios, together with values (within parentheses) from the sum of sequence analysis.…”

Section: Methodsmentioning

confidence: 99%

“…1, [6]), which are purified by reverse-phase HPLC, using a gradient of acetonitrile in O.lolo trifluoroacetic acid [6] Sum 112 9 11 80 17 Results from acid hydrolysis for 24 h are given in molar ratios, together with values (within parentheses) from the sum of sequence analysis. Met was analyzed as homoserine in the hydrolysates of the CNBr fragments, Sephadex G-100 (peak 2, fig.1 [6]).…”

Section: Methodsmentioning

confidence: 99%

“…Camel milk whey proteins were separated into four fractions by Sephadex G-100 chromatography as described [6]. The whey protein lacking cysteine was further separated by reverse-phase HPLC ( fig.…”

Section: Isolationmentioning

confidence: 99%

“…INTRODUCTION Milk whey contains proteins of different types which have been characterized from several species. Such proteins include cY-lactalbumin, found in milk of all ruminants and non-ruminants so far studied (a recent addition in [l]); ,& lactoglobulin, which appears to be mostly confined to ruminants and some non-ruminants, but its presence in some species is controversial [2,3]; a half-cystine rich protein characterized from rat, mouse [4,5] and camel [6,7] milk; and casein and its fragments. &Casein, well studied in.…”

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Characterization of a heterogeneous camel milk whey non‐casein protein

et al. 1987

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A milk protein, occurring in the whey fraction, has been characterized from camel milk. Determination of the primary structure reveals the existence of two related types of chain with residue differences in at least the N-terminal region. A fragment representing an N-terminal part of the protein was also recovered (heterogeneous at the same positions). The absence of cysteine residues in the protein shows that no disulphide bridges are present. The pattern of fragments and a parent protein resembles that for casein and its fragments, showing that fragments and a multiplicity of forms may be typical for different milk proteins.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Isolationmentioning

confidence: 99%

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Characterization of a heterogeneous camel milk whey non‐casein protein

et al. 1987

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“…Whey acidic protein (WAP) is the major whey protein in the milk of mice, rats, rabbits and camels [1][2][3][4][5][6][7][8]; it has not been found in the milk of swine or other livestock. The WAP gene possesses powerful regulatory elements and its expression is regulated by a complex interaction of several lactogenic hormones [9].…”

Section: Introductionmentioning

confidence: 99%

A Transgenic Mouse Model for Investigating the Response of the Upstream Region of Whey Acidic Protein (WAP) Gene to Various Steroid Hormones

et al. 2001

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Abstract:The limitations of studies of clarification of response elements of whey acidic protein (WAP) gene to hormones using mammary cell lines has been shown. We studied the response of the upstream region (2.6 kb) of WAP to various steroid hormones using gonadectomized mWAP/hGH transgenic mice. Ovariectomy or castration for transgenic mice was performed at 10 days or 30 days post partum. Various steroid hormones were administered daily for 10 days to the gonadectomized transgenic mice after they reached 2 months of age. Prior to the hormonal administration and 24 hr after the final administration, blood was collected and the hGH levels in the plasma was measured by RIA. Daily doses of estradiol-17β were significantly more effective at increasing hGH levels in transgenic females ovariectomized at 10 days post partum than progesterone of an equal dose. A combined dose of progesterone and of estradiol-17β significantly amplified the increase of hGH levels accompanied by the great development of mammary glands, compared to a dose of progesterone alone. Corticosterone induced only a slight increase of hGH, while testosterone had no effect. The doses of gonadal steroid hormones did not induce an increase in hGH levels and development of mammary glands in the castrated transgenic males. The results showed that the response of 5' region of WAP requires at least some extended development of the mammary gland and that the 2.6 kb upstream region of the exogenous WAP gene contained the element responsive to ovarian hormones.

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Camel Milk

El-Agamy¹

2006

Handbook of Milk of Non‐Bovine Mammals

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A small camel-milk protein rich in cysteine/half-cystine

Cited by 28 publications

References 5 publications

Characterization of a heterogeneous camel milk whey non‐casein protein

Characterization of a heterogeneous camel milk whey non‐casein protein

A Transgenic Mouse Model for Investigating the Response of the Upstream Region of Whey Acidic Protein (WAP) Gene to Various Steroid Hormones

Camel Milk

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