A single transcript CRISPR/Cas9 mediated mutagenesis of CaERF28 confers anthracnose resistance in chilli pepper (Capsicum annuum L.)

Mishra, Rukmini; Mohanty, Jatindra Nath; Mahanty, Bijayalaxmi; Joshi, Raj Kumar

doi:10.1007/s00425-021-03660-x

Cited by 42 publications

(18 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…On the other hand, the application of this approach can be limited by the scarce knowledge on the genetic mechanisms regulating plant response to pathogens, which, in case of Colletotrichum are not totally uncovered. To date, the only scientific evidence on the efficacy of NBT application against this disease was reported by Mishra and colleagues [ 125 ] who developed CRISPR-Cas9 T-DNA-free homozygous pepper plants ( Capsicum annum ) harboring a desired mutation in the CaERF28 gene. The expression of this gene leads to the downregulation of the endogenous defense mechanisms [ 126 , 127 ] and transformed plants were significantly less susceptible to C. truncatum .…”

Section: Innovative and Sustainable Approachesmentioning

confidence: 99%

Management of Post-Harvest Anthracnose: Current Approaches and Future Perspectives

Ciofini

Negrini

Baroncelli

et al. 2022

Plants

View full text Add to dashboard Cite

Anthracnose is a severe disease caused by Colletotrichum spp. on several crop species. Fungal infections can occur both in the field and at the post-harvest stage causing severe lesions on fruits and economic losses. Physical treatments and synthetic fungicides have traditionally been the preferred means to control anthracnose adverse effects; however, the urgent need to decrease the use of toxic chemicals led to the investigation of innovative and sustainable protection techniques. Evidence for the efficacy of biological agents and vegetal derivates has been reported; however, their introduction into actual crop protection strategies requires the solutions of several critical issues. Biotechnology-based approaches have also been explored, revealing the opportunity to develop innovative and safe methods for anthracnose management through genome editing and RNA interference technologies. Nevertheless, besides the number of advantages related to their use, e.g., the putative absence of adverse effects due to their high specificity, a number of aspects remain to be clarified to enable their introduction into Integrated Pest Management (IPM) protocols against Colletotrichum spp. disease.

show abstract

Section: Innovative and Sustainable Approachesmentioning

confidence: 99%

Management of Post-Harvest Anthracnose: Current Approaches and Future Perspectives

Ciofini

Negrini

Baroncelli

et al. 2022

Plants

View full text Add to dashboard Cite

show abstract

“…Genome-editing technologies are enabling researchers to reproduce directly in elite lines the allelic variations responsible for improvement of the traits of interest ( Rothan et al., 2019 ), saving years during the breeding process and avoiding linkage drag of undesired genes/alleles ( Deng et al., 2018 ; Zhu et al., 2018 ). Some examples of its application in Solanaceae encompass the rewiring of the GABA metabolic pathway in tomato ( Li et al., 2018b ), the generation of knock-out mutants with reduced PPO activity and browning of eggplant’s fruit flesh ( Maioli et al., 2020 ), and the enhancement of resistance against anthracnose in pepper ( Mishra et al., 2021 ). CRISPR/Cas9 has been successfully applied to edit the phenylpropanoid pathway in tomato.…”

Section: Breeding Strategies For Improving Phenolics Content In Tomat...mentioning

confidence: 99%

Genetics and breeding of phenolic content in tomato, eggplant and pepper fruits

et al. 2023

View full text Add to dashboard Cite

Phenolic acids and flavonoids are large groups of secondary metabolites ubiquitous in the plant kingdom. They are currently in the spotlight due to the numerous health benefits associated with their consumption, as well as for their vital roles in plant biological processes and in plant-environment interaction. Tomato, eggplant and pepper are in the top ten most consumed vegetables in the world, and their fruit accumulation profiles have been extensively characterized, showing substantial differences. A broad array of genetic and genomic tools has helped to identify QTLs and candidate genes associated with the fruit biosynthesis of phenolic acids and flavonoids. The aim of this review was to synthesize the available information making it easily available for researchers and breeders. The phenylpropanoid pathway is tightly regulated by structural genes, which are conserved across species, along with a complex network of regulatory elements like transcription factors, especially of MYB family, and cellular transporters. Moreover, phenolic compounds accumulate in tissue-specific and developmental-dependent ways, as different paths of the metabolic pathway are activated/deactivated along with fruit development. We retrieved 104 annotated putative orthologues encoding for key enzymes of the phenylpropanoid pathway in tomato (37), eggplant (29) and pepper (38) and compiled 267 QTLs (217 for tomato, 16 for eggplant and 34 for pepper) linked to fruit phenolic acids, flavonoids and total phenolics content. Combining molecular tools and genetic variability, through both conventional and genetic engineering strategies, is a feasible approach to improve phenolics content in tomato, eggplant and pepper. Finally, although the phenylpropanoid biosynthetic pathway has been well-studied in the Solanaceae, more research is needed on the identification of the candidate genes behind many QTLs, as well as their interactions with other QTLs and genes.

show abstract

“…Recently, an enhance resistance to anthracnose caused by Colletotrichum spp. was observed following a single transcript CRISPR/Cas9 modification of CaERF28 , a major susceptibility gene ( Mishra et al, 2021 ). One of the key issues that needs to be addressed is the absence of a stable regeneration system for the Capsicum .…”

Section: The “Panomics” Platform: Integrating Multi-omics Tools For T...mentioning

confidence: 99%

Chile Pepper (Capsicum) Breeding and Improvement in the “Multi-Omics” Era

et al. 2022

View full text Add to dashboard Cite

Chile pepper (Capsicum spp.) is a major culinary, medicinal, and economic crop in most areas of the world. For more than hundreds of years, chile peppers have “defined” the state of New Mexico, USA. The official state question, “Red or Green?” refers to the preference for either red or the green stage of chile pepper, respectively, reflects the value of these important commodities. The presence of major diseases, low yields, decreased acreages, and costs associated with manual labor limit production in all growing regions of the world. The New Mexico State University (NMSU) Chile Pepper Breeding Program continues to serve as a key player in the development of improved chile pepper varieties for growers and in discoveries that assist plant breeders worldwide. Among the traits of interest for genetic improvement include yield, disease resistance, flavor, and mechanical harvestability. While progress has been made, the use of conventional breeding approaches has yet to fully address producer and consumer demand for these traits in available cultivars. Recent developments in “multi-omics,” that is, the simultaneous application of multiple omics approaches to study biological systems, have allowed the genetic dissection of important phenotypes. Given the current needs and production constraints, and the availability of multi-omics tools, it would be relevant to examine the application of these approaches in chile pepper breeding and improvement. In this review, we summarize the major developments in chile pepper breeding and present novel tools that can be implemented to facilitate genetic improvement. In the future, chile pepper improvement is anticipated to be more data and multi-omics driven as more advanced genetics, breeding, and phenotyping tools are developed.

show abstract

A single transcript CRISPR/Cas9 mediated mutagenesis of CaERF28 confers anthracnose resistance in chilli pepper (Capsicum annuum L.)

Cited by 42 publications

References 52 publications

Management of Post-Harvest Anthracnose: Current Approaches and Future Perspectives

Management of Post-Harvest Anthracnose: Current Approaches and Future Perspectives

Genetics and breeding of phenolic content in tomato, eggplant and pepper fruits

Chile Pepper (Capsicum) Breeding and Improvement in the “Multi-Omics” Era

Contact Info

Product

Resources

About