A Simplified Procedure for Hematopoietic Stem Cell Amplification Using a Serum-Free, Feeder Cell-Free Culture System

Rogers, Ian; Yamanaka, Yojiro; Casper, Robert F.

doi:10.1016/j.bbmt.2008.06.002

Cited by 13 publications

(15 citation statements)

References 53 publications

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“…TNCs, Lin- or Lin+ cells from human umbilical cord blood or human bone marrow were used for our xenograft studies. NOD/SCID mice were prepared and transplanted as per standard protocols [11], [24], [25]. Our standard engraftment time of 8–10 weeks was used in the preliminary set of results.…”

Section: Resultsmentioning

confidence: 99%

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Bone Marrow Transplantation Results in Human Donor Blood Cells Acquiring and Displaying Mouse Recipient Class I MHC and CD45 Antigens on Their Surface

et al. 2009

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BackgroundMouse models of human disease are invaluable for determining the differentiation ability and functional capacity of stem cells. The best example is bone marrow transplants for studies of hematopoietic stem cells. For organ studies, the interpretation of the data can be difficult as transdifferentiation, cell fusion or surface antigen transfer (trogocytosis) can be misinterpreted as differentiation. These events have not been investigated in hematopoietic stem cell transplant models.Methodology/Principal FindingsIn this study we investigated fusion and trogocytosis involving blood cells during bone marrow transplantation using a xenograft model. We report that using a standard SCID repopulating assay almost 100% of the human donor cells appear as hybrid blood cells containing both mouse and human surface antigens.Conclusion/SignificanceHybrid cells are not the result of cell-cell fusion events but appear to be due to efficient surface antigen transfer, a process referred to as trogocytosis. Antigen transfer appears to be non-random and includes all donor cells regardless of sub-type. We also demonstrate that irradiation preconditioning enhances the frequency of hybrid cells and that trogocytosis is evident in non-blood cells in chimera mice.

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Section: Resultsmentioning

confidence: 99%

“…The use of human cells allowed us to distinguish between human cells (HLA:ABC+) and mouse cells (H2Kd+). Hybrid cells would be positive for both human and mouse antigens [11], [12].…”

Section: Introductionmentioning

confidence: 99%

Bone Marrow Transplantation Results in Human Donor Blood Cells Acquiring and Displaying Mouse Recipient Class I MHC and CD45 Antigens on Their Surface

et al. 2009

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“…Therefore, it frequently happens that the UCB nucleated cell dose does not reach the lower acceptable limit for an adult, though making it suitable for most of pediatric patients [9]. Several approaches have been proposed to cope with this inconvenience, viz infusion of units from multiple donors [10][11][12], ex vivo or in vitro cell culturing prior to transplantation [13][14][15][16][17][18][19], non-myeloablative conditioning regimens [20], intra-bone inoculation [21].…”

Section: Introductionmentioning

confidence: 99%

In Silico Analyses of Proteomic Data Suggest a Role for Heat Shock Proteins in Umbilical Cord Blood Hematopoietic Stem Cells

D’Alessandro

Grazzini

Giardina

et al. 2010

Stem Cell Rev and Rep

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Umbilical-cord blood (UCB) has growingly become an accepted alternative source of hematopoietic stem cells for transplantation purposes. However, the low cell dose limit within a single unit is still an obstacle hindering the way of a broader diffusion. The real deal is the lack of knowledge about the molecular processes governing the events of expansion and differentiation of these cells. In order to fill this void, several studies were focused on the identification of the peculiar whole protein profile of UCB-derived hematopoietic stem cells. In this review article we provide a referenced list of overall proteins from UCB-derived hematopoietic stem and progenitor cells. This list has been elaborated for pathway and network analyses, along with GO term enrichment for biological and molecular functions, in order to individuate main classes of proteins governing functioning of these cells. From these analyses it seems to emerge a central role for heat shock proteins in immature hematopoietic stem cells. Their role might be relevant in protecting crucial transcription factors which drive proliferation and differentiation towards a specific lineage (e.g. erythroid, myeloid). Hereby we also stress the helpfulness of interactomics elaboration in providing a unified overview of independent proteomics data. It appears that maturation, other than representing a bottleneck to protein expression, could sculpt interaction maps via reducing complexity of immature interactomics profiles.

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“…MSC are defined by the presence of a number of surface antigens and a recent study suggests that these cell arise in the neural crest [22]. In addition it has been demonstrated that there is a lineage negative CD45+ cell population in cord blood which may also have multi-potential capabilities [27].…”

Section: The Development Of Regenerative Medicinementioning

confidence: 99%

Private Cord Blood Banking: Current Use and Clinical Future

Hollands

McCauley

2009

Stem Cell Rev and Rep

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International private umbilical cord blood banking has expanded rapidly in recent years since the first cord blood transplant which was 20 years ago. Private companies offer parents the opportunity to store umbilical cord blood for the possible future use by their child or other family members. The private cord blood industry has been criticised by a number of professional bodies including the EU Ethics Committee, the Royal College of Obstetrics and Gynaecology, the Royal College of Midwives and the US College of Paediatrics. This review presents the arguments from the opponents of private cord blood banking, and then makes the case for private cord banking based on the latest scientific and clinical evidence.

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A Simplified Procedure for Hematopoietic Stem Cell Amplification Using a Serum-Free, Feeder Cell-Free Culture System

Cited by 13 publications

References 53 publications

Bone Marrow Transplantation Results in Human Donor Blood Cells Acquiring and Displaying Mouse Recipient Class I MHC and CD45 Antigens on Their Surface

Bone Marrow Transplantation Results in Human Donor Blood Cells Acquiring and Displaying Mouse Recipient Class I MHC and CD45 Antigens on Their Surface

In Silico Analyses of Proteomic Data Suggest a Role for Heat Shock Proteins in Umbilical Cord Blood Hematopoietic Stem Cells

Private Cord Blood Banking: Current Use and Clinical Future

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