A simple technique for fat biopsy of PBB-exposed individuals.

Abstract

A simple nonsurgical technique of obtaining fat samples by aspiration from the gluteal prominence was developed by Hirsh in 1960 and has been in use in our Nutrition Clinic at the Mount Sinai Hospital for several years. We have modified it for field use and the analysis of fat-soluble hydrocarbon residues. All the materials which will contain the fat sample to be analyzed are washed with acetone and pesticide residue-free hexane, and a 15 gauge needle and 33 cc syringe are sterilized. Aspiration of fat from the lateral gluteal prominence is accomplished under local xylocaine anesthetic. The anesthetic also serves as the vehicle into which the fat is broken by the shearing action of the 15 gauge needle. Fat particles are sucked into the syringe by a constant vacuum kept on the syringe during lateral movement of the needle under (and parallel to) the skin within the gluteal fat pad; 200--500 ml of fat can be obtained for hydrocarbon residue analysis. The only complications have been some mild hematomas at the site of the aspiration. The method avoids surgical biopsy and sutures and takes about 7--8 min.ImagesFIGURE 1. AFIGURE 1. BFIGURE 2.FIGURE 3.

A novel biopsy method to increase yield of subcutaneous abdominal adipose tissue

“…This novel abdominal fat biopsy procedure used a Bergström side-cutting biopsy needle and yielded an average of 1.5 grams of intact SAT, which was several fold greater than what has been previously reported using punch biopsy and needle aspiration techniques (14–16). Additionally, to our knowledge, we are the first to report the ability to safely and consistently sample adipose tissue from the dSAT compartment by a percutaneous bedside approach.…”

“…Since sSAT is easily accessible and can reflect some biological aspects of VAT (9,13), non-invasive aspiration and punch biopsy procedures are used to sample this depot (2,13). However, these methods typically acquire only 100–500 milligrams of sSAT (14–16), far less than what is needed for research studies that may include fluorescent activated cell sorting, RNA extraction, ex vivo expression of molecular and post-translational protein mediators, and histology. Further, some of these techniques, including liposuction type approaches, require vigorous suctioning, which may disrupt the integrity of adipose tissue for multiple, and complex research studies.…”

Para-dichlorobenzene toxicity – a review of potential neurotoxic manifestations

“…Importantly, bioaccumulation in sediments and food crops has been shown to occur and has led to contamination of aquatic species that are used for food consumption and is The rate of metabolism of PDCB by CYP 2E1 enzymes can potentially be increased by various enzyme inducers including acetaminophen, isoniazid and ethanol [Flockhart et al 2007, Kim et al 2007]. (C) Serum level of the PDCB may be reduced by hemodialysis [Daum et al 1978]. (D) The phenomenon of coasting due to mobilization of fat stores of PDCB following cessation of oral ingestion can be regulated by prevention of starvation (adequate diet or tube feeds) and use of hormones preventing lipolysis in adipose tissue through administration of insulin [Herron et al 2002;Schweigert et al 2002].…”

“…Nonsurgical techniques of obtaining fat samples by aspiration from the gluteal prominence have been reported by several investigators. Daum and colleagues [1978] modified this technique for the analysis of fatsoluble hydrocarbon residues [Daum et al 1978]. Aspiration of fat from the lateral gluteal prominence is performed under local anesthetic, which makes this a potentially feasible procedure.…”

New device technologies for subcutaneous fat biopsy

“…Subcutaneous fat biopsy has become the standard for the diagnosis of systemic amyloidosis, for metabolic, immunologic, endocrine, and genetic studies of adipose tissue, and for measuring lipophilic environmental contaminants [3–7]. Most practioners use a 10 or 20 ml syringe, a 16 to 18 gauge large bore needle, and provide vacuum by pulling back the plunger on the syringe to perform multiple passes until enough sample has been obtained [2–10].…”