A simple selection system for construction of recombinant gD-negative pseudorabies virus as a vaccine vector

Shiau, Ai‐Li; Liu, Chia Wen; Wang, Shiang Yiu; Tsai, Chiau Yuang; Wu, Chao‐Liang

doi:10.1016/s0264-410x(01)00426-1

Cited by 8 publications

(13 citation statements)

References 22 publications

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“…The mouse Oct-3/4 cDNA was obtained by reverse transcription-PCR (RT-PCR) amplification of mRNA from MBT-2 cells. The resulting 975-bp fragment cloned into pGEM T-Easy was excised from the vector by SpeI and EcoRI digestions and subcloned into the XbaI and EcoRI sites of pTCY (17), resulting in pTCY-Oct-3/4.…”

Section: Methodsmentioning

confidence: 99%

“…To determine whether exogenous expression of Oct-3/4 enhanced the transcriptional activity of 9ÂORE-CMVmini promoter in Oct-3/4 nonexpressing cells, NIH3T3 cells were cotransfected with 1 Ag of pFRL2-9ÂORE-CMVmini and pTCY-Oct-3/4 (0.8, 0.6, 0.4, 0.2, and 0 Ag). The total amount of plasmid DNA for transfection was kept constant by adding pTCY-Lac (17). Cell lysates were harvested for assessing luciferase activities as described above.…”

Section: Methodsmentioning

confidence: 99%

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Tumor-Selective Replication of an Oncolytic Adenovirus Carrying Oct-3/4 Response Elements in Murine Metastatic Bladder Cancer Models

Wu¹,

Shieh

Chang³

et al. 2008

Clinical Cancer Research

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Purpose: Oncolytic adenoviruses are attractive therapeutics for cancer because they selectively replicate in tumors. However, targeting tumor metastasis remains a major challenge for current virotherapy for cancer. Oct-3/4 is specifically expressed in embryonic stem cells and tumor cells. Oct-3/4 highly expressed in cancer cells may be a potential target for cancer therapy. We developed an E1B-55 kDa–deleted adenovirus, designated Ad.9OC, driven by nine copies of Oct-3/4 response element for treating Oct-3/4–expressing metastatic bladder cancer. Experimental Design: We examined the expression of Oct-3/4 in human bladder tumor tissues and bladder cancer cell lines. We also evaluated the cytolytic and antitumor effects of Ad.9OC on bladder cancer cells in vitro and in vivo. Results: Oct-3/4 expression was detected in bladder cancer cell lines, as well as in human bladder tumor tissues. Notably, Oct-3/4 expression was higher in metastatic compared with nonmetastatic bladder cancer cells. Ad.9OC induced higher cytolytic activity in metastatic bladder cancer cells than in their nonmetastatic counterparts, whereas it did not cause cytotoxicity in normal cells. Pharmacologic and short hairpin RNA–mediated Oct-3/4 inhibition rendered bladder cancer cells more resistant to Ad.9OC-induced cytolysis. Replication of Ad.9OC was detected in murine bladder cancer cells and bladder tumor tissues. We also showed the effectiveness of Ad.9OC for treating bladder cancer in subcutaneous, as well as metastatic, bladder tumor models. Conclusions: Ad.9OC may have therapeutic potential for treating Oct-3/4–expressing tumors. Especially, metastatic bladder tumors are good target for Ad.9OC treatment. Because Oct-3/4 is expressed in a broad spectrum of cancers, Ad.9OC may be broadly applicable.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Tumor-Selective Replication of an Oncolytic Adenovirus Carrying Oct-3/4 Response Elements in Murine Metastatic Bladder Cancer Models

Wu¹,

Shieh

Chang³

et al. 2008

Clinical Cancer Research

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“…PrV strain TNL is a virulent wt strain of PrV, while CW1 virus, derived from strain TNL, is a gD-and gE-negative and herpes simplex virus type 1 (HSV-1) TK-positive PrV strain (33). The TK expression cassette within the CW1 virus can be conveniently replaced with the AAV rep and cap genes by homologous recombination.…”

Section: Methodsmentioning

confidence: 99%

“…The TK expression cassette within the CW1 virus can be conveniently replaced with the AAV rep and cap genes by homologous recombination. Vero (African green monkey kidney cells), Vero-gD (PrV gD-overexpressing Vero cells) (33), 293 (human embryonic kidney cells), BHK (baby hamster kidney cells), and NIH 3T3 (mouse fibroblasts) cells were cultured in Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine serum (or calf serum for NIH 3T3 cells), 2 mM L-glutamine, and 50 g of gentamicin/ml. Female C3H/HeNCrj mice (6 to 8 weeks old) were obtained from the Laboratory Animal Center of the National Cheng Kung University Medical College.…”

Section: Methodsmentioning

confidence: 99%

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Novel Strategy for Generation and Titration of Recombinant Adeno-Associated Virus Vectors

Shiau

Liu

2005

J Virol

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Recombinant adeno-associated virus (rAAV) vectors have many advantages for gene therapeutic applications compared with other vector systems. Several methods that use plasmids or helper viruses have been reported for the generation of rAAV vectors. Unfortunately, the preparation of large-scale rAAV stocks is labor-intensive. Moreover, the biological titration of rAAV is still difficult, which may limit its preclinical and clinical applications. For this study, we developed a novel strategy to generate and biologically titrate rAAV vectors. A recombinant pseudorabies virus (PrV) with defects in its gD, gE, and thymidine kinase genes was engineered to express the AAV rep and cap genes, yielding PS virus, which served as a packaging and helper virus for the generation of rAAV vectors. PS virus was useful not only for generating high-titer rAAV vectors by cotransfection with an rAAV vector plasmid, but also for amplifying rAAV stocks. Notably, the biological titration of rAAV vectors was also feasible when cells were coinfected with rAAV and PS virus. Based on this strategy, we produced an rAAV that expresses prothymosin ␣ (ProT). Expression of the ProT protein in vitro and in vivo mediated by rAAV/ProT gene transfer was detected by immunohistochemistry and a bioassay. Taken together, our results demonstrate that the PrV vector-based system is useful for generating rAAV vectors carrying various transgenes.

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Mitochondrial ribosomal protein S36 delays cell cycle progression in association with p53 modification and p21^WAF1/CIP1 expression

Chen

Chang

Shiau

et al. 2006

J of Cellular Biochemistry

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Ribosomal biogenesis is correlated with cell cycle, cell proliferation, cell growth and tumorigenesis. Some oncogenes and tumor suppressors are involved in regulating the formation of mature ribosome and affecting the ribosomal biogenesis. In previous studies, the mitochondrial ribosomal protein L41 was reported to be involved in cell proliferation regulating through p21(WAF1/CIP1) and p53 pathway. In this report, we have identified a mitochondrial ribosomal protein S36 (mMRPS36), which is localized in the mitochondria, and demonstrated that overexpression of mMRPS36 in cells retards the cell proliferation and delays cell cycle progression. In addition, the mMRPS36 overexpression induces p21(WAF1/CIP1) expression, and regulates the expression and phosphorylation of p53. Our result also indicate that overexpression of mMRPS36 affects the mitochondrial function. These results suggest that mMRPS36 plays an important role in mitochondrial ribosomal biogenesis, which may cause nucleolar stress, thereby leading to cell cycle delay.

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A simple selection system for construction of recombinant gD-negative pseudorabies virus as a vaccine vector

Cited by 8 publications

References 22 publications

Tumor-Selective Replication of an Oncolytic Adenovirus Carrying Oct-3/4 Response Elements in Murine Metastatic Bladder Cancer Models

Tumor-Selective Replication of an Oncolytic Adenovirus Carrying Oct-3/4 Response Elements in Murine Metastatic Bladder Cancer Models

Novel Strategy for Generation and Titration of Recombinant Adeno-Associated Virus Vectors

Mitochondrial ribosomal protein S36 delays cell cycle progression in association with p53 modification and p21^WAF1/CIP1 expression

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A simple selection system for construction of recombinant gD-negative pseudorabies virus as a vaccine vector

Cited by 8 publications

References 22 publications

Tumor-Selective Replication of an Oncolytic Adenovirus Carrying Oct-3/4 Response Elements in Murine Metastatic Bladder Cancer Models

Tumor-Selective Replication of an Oncolytic Adenovirus Carrying Oct-3/4 Response Elements in Murine Metastatic Bladder Cancer Models

Novel Strategy for Generation and Titration of Recombinant Adeno-Associated Virus Vectors

Mitochondrial ribosomal protein S36 delays cell cycle progression in association with p53 modification and p21WAF1/CIP1 expression

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Product

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Mitochondrial ribosomal protein S36 delays cell cycle progression in association with p53 modification and p21^WAF1/CIP1 expression